Saliva DNA Isolation Reagent Kit (up to 4 mL) For the isolation of high quality DNA from up to 4 mL of preserved saliva samples within a laboratory setting For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Saliva DNA Isolation Reagent Kit (up to 4 mL) For the isolation of high quality DNA from up to 4 mL of preserved saliva samples within a laboratory setting Price USD$125.00 SKU RU35720 Size 50 Preps Quantity Overview Details Documentation FAQs Citations Overview Cost effective method to isolate DNA from preserved saliva samples collected using Norgen's saliva DNA devices or other preservation methodsIsolate DNA from a range of input volumes - up to 4 mLIsolate DNA of high recovery and quality suitable for sensitive downstream applications including PCR, qPCR, sequencing, SNP analysis, microarrays, RFLP and Southern Blot Analysis Norgen's Saliva DNA Isolation Reagent Kit (up to 4 mL) is designed for isolation of high quality DNA from up to 4 mL of preserved saliva samples within a laboratory setting. The Saliva DNA Isolation Reagent Kit (up to 4 mL) contains enough reagents for 50 preps of 4 mL saliva samples preserved via Norgen Biotek's Saliva DNA Collection and Preservation Devices (RU49000), or other preservation methods. Details SHELF LIFE AND HANDLING The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at -20°C. The Proteinase K is a white turbid solution. A white precipitate is formed upon storage. Make sure to re-suspend the white precipitate by vortexing before use. This kit is stable for 2 years after the date of shipment. Component Cat. RU35720 (50 preps) Proteinase K in Storage Buffer 8 mL Binding Buffer B 85 mL Product Insert 1 Documentation PROTOCOLS (RU35720) Saliva DNA Isolation Reagent Kit - Protocol (50 prep) FLYERS Flyer FAQs How to process preserved saliva volumes less than 4 mL? The procedure can be scaled down for smaller volumes. The kit manual includes a table to help with calculations. What buffer should I use to resuspend the DNA pellet? The final DNA pellet should be resuspended in DNase-free TE buffer, which is not supplied with the kit. How to get higher concentration of saliva DNA from the Saliva DNA Isolation Reagent kit? To obtain a higher concentration of DNA, please use half the volume of TE buffer mentioned in calculation table (refer to product manual). It is not recommended to use lower than half the volume of TE buffer mentioned in the calculation table. How to properly dissolve the DNA pellet? Large amounts of high molecular DNA can be very slow to rehydrate (dissolve) completely. Incubate at 55 °C for 30 minutes with occasional vortexing to aid in the process. Incomplete rehydration of the DNA will affect estimating the DNA concentration and can result in failure of downstream applications such as SNP, digestion and PCR. Can the Saliva DNA Isolation Reagent kit be used with fresh saliva samples? Saliva DNA Isolation Reagent kit is optimized to work with saliva preserved with chemical preservatives only, for example, saliva preserved with Norgen's Saliva DNA collection and preservation devices. To isolate DNA from fresh saliva samples, please use our Saliva DNA isolation kit which can process both fresh and preserved saliva samples. Citations Title Alterations to the gut microbiome after sport-related concussion in a collegiate football players cohort: A pilot study Journal Brain Behav Immun Health. 2022. Authors Soriano S, Curry K, Sadrameli SS, Wang Q, Nute M, Reeves E, Kabir R, Wiese J, Criswell A, Schodrof S, Britz GW, Gadhia R, Podell K, Treangen T, Villapol S Related Products Shipping Accessories Saliva DNA Collection and Preservation Devices Saliva DNA Isolation Kit
Saliva DNA Isolation Reagent Kit (up to 4 mL) For the isolation of high quality DNA from up to 4 mL of preserved saliva samples within a laboratory setting For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Saliva DNA Isolation Reagent Kit (up to 4 mL) For the isolation of high quality DNA from up to 4 mL of preserved saliva samples within a laboratory setting Price USD$125.00 SKU RU35720 Size 50 Preps Quantity