AAV Quantification Kit
AAV Quantification Kit
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Supporting Data
Figure 1. Amplification of ten fold serial dilutions of purified Norgen AAV Standard: 10-2 to 10-8 dilutions and no target control (NTC).
Figure 2. XY Scatter Plot of Ct versus log copy number demonstrates high linearity for AAV standard dilution series from 10-2 dilution to 10-8 dilution (copy number of 2,000 to 2,000,000,000).
Figure 3. A) Determination of copy number using Norgen's AAV Quantification Kit for samples purified with Norgen's AAV Purification Kit based on 1 mL, 3 mL, or 9 mL input volumes. B) Transduction of HTX cells with 100 µL of AAV preparation from 1 mL, 3 mL, or 9 mL input volumes purified using the Norgen AAV Purification Kit.
Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.
Component | Cat. 63800 (24 rxns) |
|---|---|
Enzyme Incubation Buffer A | 500 µL |
DNase I | 25 µL |
DNase Stop Solution | 100 µL |
2X PCR Mastermix | 350 µL |
AAV Primer & Probe Mix | 90 µL |
AAV Standard | 6 µL |
Nuclease-Free Water (Negative Control) | 1.25 mL |
Product Insert | 1 |
Documentation
FAQs
The AAV quantification is a universal qPCR assay targeting the AAV2 ITR, and enables quantification of most AAV vectors. The AAV2 ITR Quant Kit has a cross reactivity for AAV1, 3, 6, and 7.
This kit can detect from approx. 2 x 10^3 to 2 x 10^10 genome copies of AAV.
This kit uses a 62 bp amplicon covering the AAV2 ITR to overcome the limitations of using a plasmid-based standard.