Soil DNA Isolation Plus Kit For the detection of microorganisms from soil samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Soil DNA Isolation Plus Kit For the detection of microorganisms from soil samples SKU 64000 Format Plus Size 50 Preps Quantity Overview Details Documentation Citations Overview Rapid and convenient method to detect microorganisms in soil samplesProcess all soil typesRemove organic substances using the OSR SolutionRemove all humic acid from DNA samplesFast and easy processing using a rapid spin-column formatIsolate high quality total DNA from a variety of microorganisms including bacteria, fungi and algae Norgen's Soil DNA Isolation Plus Kit provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid and PCR inhibitors using the provided the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation. Details Supporting Data Previous Figure 1. Comparison of DNA Yield from Top Soil and Clay Samples. Norgen’s Soil DNA Isolation Plus Kit (Cat. 64000) and Competitor M’s kit were used to isolate DNA from 250 mg of top soil and clay samples. Following isolation, 10 µL from each 100 µL elution was loaded on 1% TAE agarose gel. Lane M: Norgen's HighRanger 1kb DNA Ladder. Figure 2. Comparison of DNA Concentration Isolated from Top Soil and Clay Samples. Norgen’s Soil DNA Isolation Plus Kit (Cat. 64000) and Competitor M’s kit were used to isolate DNA from 250 mg top soil and clay samples. Norgen’s kit showed higher DNA concentrations for both samples in comparison to the competitor kit. Figure 3. High Quality DNA confirmed by Real-time PCR. Soil DNA was isolated from 250 mg of clay (containing low humic acids) and top soil (containing high humic acids) samples using Norgen's Soil DNA Isolation Plus Kit and Competitor M's Kit. DNA quality was confirmed by Real-time PCR using 4 µL of soil DNA (total PCR reaction volume was 20 µL) to detect 16s rDNA from different soil samples. The earlier Ct value with Norgen's DNA samples (red lines) compared to Competitor M's samples (green lines) indicated a higher quality of soil DNA for downstream applications. Figure 4. Microbial profiles from soil samples (clay and top soil) isolated using Norgen’s Soil DNA Isolation Plus Kit (Cat. 64000) and Competitor M's kit. The relative abundance of phylum-level classifications indicates the efficiency of DNA isolation and the qualityof DNA. 16S rRNA genes (v3-4 region) were amplified and the amplicons were sequenced on Illumina® MiSeq™ (MiSeq Reagent Kit v3) using Norgen’s 16S Metagenomic workflow with Illumina 16S Metagenomics Pipeline (v1.0.1). Figure 5. Abundance of bacterial genera heat map based on phylum level between two DNA soil samples isolation methods; Norgen’s Soil DNA Isolation Plus Kit (Cat. 64000) and Competitor M's kit. The numbers indicate species count. Next Click for expanded view Kit Specifications Maximum Soil Input 250 mg Type of Soil Processed All soil types Maximum Column Binding Capacity 50 μg Maximum Column Loading Volume 650 μL Time to Complete 10 Purifications 30 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment. ComponentCat. 64000 (50 preps)Lysis Buffer G45 mLLysis Additive A2 x 6 mLBinding Buffer I7 mLOSR Solution3 mLLysis Buffer QP25 mLWash Solution A18 mLElution Buffer B8 mLBead Tubes50Spin Columns50Collection Tubes50Elution Tubes (1.7 mL)50Product Insert1 Documentation PROTOCOLS (64000) Soil DNA Isolation Plus Kit - Protocol (50 prep) APPLICATION NOTES The Effect of Sample Preparation on Humic Acid Removal from Peat Samples: A Comparative Study Norgen’s Soil DNA Isolation Kit Outperforms a Competitor with Effective Humic Acid Removal for Metagenomic Studies SAFETY DATA SHEETS (64000) Soil DNA Isolation Plus Kit - Safety Data Sheet POSTERS A Novel Technology to Extract Nucleic Acids from Different Environmental Samples including Plants and Soil FLYERS Flyer Citations Title Enhancing bioelectrochemical hydrogen production from industrial wastewater using Ni-foam cathodes in a microbial electrolysis cell pilot plant Journal Water Research. 2024. Authors Oscar Guerrero-Sodric, Juan Antonio Baeza, Albert Guisasola Title Coupling anammox and heterotrophic denitrification activity at mainstream conditions in a single reactor unit Journal Chemical Engineering Journal. 2022. Authors Xènia Juan-Díaz, Lluc Olmo, Julio Pérez, Julián Carrera Title Comparative Metagenomics of Anaerobic Digester Communities Reveals Sulfidogenic and Methanogenic Microbial Subgroups in Conventional and Plug Flow Residential Septic Tank Systems Journal Processes. 2022. Authors James Naphtali, Alexander W. Y. Chan, Faizan Saleem, Enze Li, Jacob Devries and Herb E. Schellhorn Title Ceramic-supported graphene oxide membrane bioreactor for the anaerobic decolorization of azo dyes Journal Journal of Water Process Engineering. 2022. Authors Mohammad Shaiful Alam Amin, Frank Stüber, Jaume Giralt, Agustí Fortuny, Azael Fabregat, Josep Font Title Evaluating the production and bio-stimulating effect of 5-methyl 1, hydroxy phenazine on microbial fuel cell performance Journal International Journal of Environmental Science and Technology. 2017. Authors S. Yousaf, M. Anam, N. Ali Title Comparison of Total DNA Extraction Methods for Microbial Community from Polluted Soil Journal Agriculture and Agricultural Science Procedia. 2015. Authors Ana-Maria Tanase, Ioana Mereuta Iulia Chiciudean, Robertina Ionescu, Ligia Milea, Călina Petruța Cornea, Tatiana Vassu, Ileana Stoica Title Microbial community distribution and genetic analysis in a sludge active treatment for a complex industrial wastewater: a study using microbiological and molecular analysis and principal component analysis Journal Annals of Microbiology. 2015. Authors Giulio Moretti, Federica Matteucci, Claudia Ercole, Francesco Vegliò, Maddalena Del Gallo Title It takes an individual plant to raise a community: TRFLP analysis of the rhizosphere microbial community of two pairs of high- and low-metal-accumulating plants. Journal Soil Biology and Biochemistry. 2015. Authors Melanie P. Columbus, Sheila M. Macfie. Title Molecular Characterization and Potential of Bacterial Species Associated with Cassava Waste Journal Nigerian Food Journal. 2014. Authors A.I. Elijah, , O.O. Atanda, A.R. Popoola, S.V.A. Uzochukwu Title Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments. Journal American Chemical Society. 2014. Authors Shirley Y. Wong, Athanasios Paschos, Radhey S. Gupta, and Herb E. Schellhorn. Title Network topology reveals high connectance levels and few key microbial genera within soils. Journal Frontiers in Environmental Science. 2014. Authors Lupatini M, Suleiman A, Jacques R, Antoniolli Z, Ferreira A, Kuramae E and Roesch L. Title Optimization and Validation of Molecular Assays for Invasive Tunicate Monitoring in Environmental Water Samples. Journal Macrothink Institute. 2013. Authors Stewart-Clark S, Davidson J, Greenwood S. Title Bioremediation of high organic load lagoon sediments: Compost addition and priming effects. Journal Chemosphere. 2012. Authors d'Errico G, Giovannelli D, Montano C, Milanovic V, Ciani M, Manini E. Title Molecular Approaches to the Study of Biological Phosphorus Cycling. Journal Soil Biology. 2010. Authors Wasaki J. and Maruyama H. Related Products Plant RNA/DNA Purification Kit
Soil DNA Isolation Plus Kit For the detection of microorganisms from soil samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Soil DNA Isolation Plus Kit For the detection of microorganisms from soil samples SKU 64000 Format Plus Size 50 Preps Quantity