UltraRanger 1 kb DNA Ladder
For research use only and NOT intended for in vitro diagnostics.
Aperçu du produit
The Norgen UltraRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our UltraRanger contains fifteen discrete fragments ranging from 300 bp to 24000 bp with higher intensity reference bands at 5000 bp and 10000 bp. This ladder is ideal for sizing digested DNA of high molecular weight.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Détails
Supporting Data
UltraRanger 1kb DNA Ladder (Cat# 12100) - 100 loads
Ladder Properties:
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Higher intensity reference bands at 5000 bp and 10000 bp
Fragment
|
Size (bp)
|
Mass (ng)
|
1
|
24000
|
30
|
2
|
14000
|
27
|
3
|
10000
|
60
|
4
|
8000
|
44
|
5
|
6000
|
36
|
6
|
5000
|
55
|
7
|
4000
|
49
|
8
|
3000
|
39
|
9
|
2500
|
35
|
10
|
2000
|
30
|
11
|
1500
|
33
|
12 | 1000 |
10 |
13 | 700 |
21 |
14 | 500 |
18 |
15 | 300 |
11 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
DNA Quantification by Gel Densitometry with Norgen DNA Ladders
Isolation of Fractionated RNA From Hela Cells Using Norgen's Cytoplasmic Nuclear RNA Purification Kit
Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative
Determining the Molecular Weight Cut-Off of Plasma Circulating DNA Using Two Commercially Available Kits
Stability of DNA Stored in Norgen’s Saliva DNA Preservative at 55°C
Isolation of Fractionated RNA From Hela Cells Using Norgen's Cytoplasmic Nuclear RNA Purification Kit
Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative
Determining the Molecular Weight Cut-Off of Plasma Circulating DNA Using Two Commercially Available Kits
Stability of DNA Stored in Norgen’s Saliva DNA Preservative at 55°C