UltraRanger 1 kb DNA Ladder
For research use only and NOT intended for in vitro diagnostics.
UltraRanger 1 kb DNA Ladder
SKU
12100
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Supporting Data
UltraRanger 1kb DNA Ladder (Cat# 12100) - 100 loads
Ladder Properties:
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Higher intensity reference bands at 5000 bp and 10000 bp
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
24000
|
30
|
|
2
|
14000
|
27
|
|
3
|
10000
|
60
|
|
4
|
8000
|
44
|
|
5
|
6000
|
36
|
|
6
|
5000
|
55
|
|
7
|
4000
|
49
|
|
8
|
3000
|
39
|
|
9
|
2500
|
35
|
|
10
|
2000
|
30
|
|
11
|
1500
|
33
|
| 12 | 1000 |
10 |
| 13 | 700 |
21 |
| 14 | 500 |
18 |
| 15 | 300 |
11 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
DNA Quantification by Gel Densitometry with Norgen DNA Ladders
Isolation of Fractionated RNA From Hela Cells Using Norgen's Cytoplasmic Nuclear RNA Purification Kit
Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative
Determining the Molecular Weight Cut-Off of Plasma Circulating DNA Using Two Commercially Available Kits
Stability of DNA Stored in Norgen’s Saliva DNA Preservative at 55°C
Isolation of Fractionated RNA From Hela Cells Using Norgen's Cytoplasmic Nuclear RNA Purification Kit
Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative
Determining the Molecular Weight Cut-Off of Plasma Circulating DNA Using Two Commercially Available Kits
Stability of DNA Stored in Norgen’s Saliva DNA Preservative at 55°C