Synovial Fluid Bacterial Genomic DNA Purification Kit
For research use only and NOT intended for in vitro diagnostics.
Synovial Fluid Bacterial Genomic DNA Purification Kit
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Supporting Data
Figure 1. Isolation and Detection of the Gram +Ve S. Aureus Bacterial Genomic DNA from 1 mL of spike-in Synovial fluid. Genomic DNA was isolated from 1 mL synovial fluid spiked with a serially diluted Gram +Ve S. Aureus using Norgen’s Synovial Fluid Bacterial genomic DNA Purification Kit. The efficiency of the purified bacterial gDNA from the spiked-in synovial fluid was evaluated against the gDNA isolation from pure culture containing the same amount of S. Aureus spiked in 1mL synovial fluid. The purified gDNA was subsequently detected using quantitative PCR. All serially diluted spiked-in Gram +Ve S. Aureus was purified with high efficiency from 1mL synovial fluid as compared to the amplification of S. Aureus from pure culture. The limit of detection for the Gram +Ve S. Aureus was down to 5 CFU/mL.
Figure 2. Isolation and Detection of the Gram -Ve E.coli Bacterial Genomic DNA from 1 mL of spike-in Synovial fluid. Genomic DNA was isolated from 1 mL synovial fluid spiked with a serially diluted Gram -Ve E.coli using Norgen’s Synovial Fluid Bacterial genomic DNA Purification Kit. The efficiency of the purified bacterial gDNA from the spiked-in synovial fluid was evaluated against the gDNA isolation from pure culture containing the same amount of E.coli spiked in 1mL synovial fluid. The purified gDNA was subsequently detected using quantitative PCR. All serially diluted spiked-in Gram -Ve E.coli was purified with high efficiency from 1mL synovial fluid as compared to the amplification of E.coli from pure culture. The limit of detection for the Gram -Ve E.coli was down to 5 CFU/mL.
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Kit Specifications
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Maximum Input
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2 x 109 bacterial cells
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Column Binding Capacity
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25 μg
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Average Yield
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up to 20 µg
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| Time to Complete 10 Purifications |
45 minutes
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Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 67900 (50 preps) |
|---|---|
| Precipitation Solution B | 15 mL |
| Lysis Buffer T | 6.5 mL |
| Solution BX | 28 mL |
| Wash Solution A | 38 mL |
| Elution Buffer B | 8 mL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |