Plant/Fungi DNA Isolation Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plant/Fungi DNA Isolation Kits SKU 26200 Format Spin Column High Throughput Magnetic Bead System High Throughput Magnetic Bead System Size 50 Preps 250 Preps Quantity Trouver un distributeur Contact Overview Details Documentation Citations Aperçu du produit These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing. Plant/Fungi DNA Isolation Kit (Spin Column) Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column. Plant/Fungi DNA Isolation 96-Well Kit (High Throughput) For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well. Plant/Fungi DNA Isolation Kit (Magnetic Bead System) The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations. Plant/Fungi DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Détails Supporting Data Previous Figure 1. Isolate DNA from a Wide Range of Plants. DNA was isolated from 50 mg samples of tobacco leaves (Lanes 1 and 2), tomato leaves (lanes 3 and 4), peach leaves (Lanes 5 and 6) and grape leaves (lanes 7 and 8) using Norgene’s Plant/Fungi DNA Isolation Kit, and 5 µL aliquots of the 100 µL elutions were run on a 1x TAE 1% agarose gel. As it can be seen, high quality DNA was isolated in all cases. The M lanes contain Norgen’s HighRanger 1Kb DNA Ladder. Figure 2. Purified DNA can be Amplified in a Real-time PCR Reaction (SYBR Green). DNA was isolated from 50 mg samples of plum and tobacco frozen leaves using Norgen’s Plant/Fungi DNA isolation Kit, and 2 µL of the eluted DNA was used in a real-time PCR reaction (total reaction volume 20 µL) with 18sr DNA primers (95°C for 3 minutes and 40 cycles at 95°C for 15 seconds and 60°C for 30 seconds). The real-time PCR was successful in amplifying both the plum and tobacco DNA, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Figure 3. Resolution of DNA isolated from four different plant species. DNA was isolated from four different plant species using Norgen’s Plant DNA Isolation Kit (Magnetic Bead System) and Norgen's Plant/Fungi DNA Isolation Kit (column format, Cat. 26200). For evaluation, 10 µL from 75 µL of elution were run on 1X TAE 1.2% agarose gel. Excellent DNA integrity and yield were observed from the Plant DNA Isolation Kit (Magnetic Bead System) (Lanes 1 to 3), indicating the robust performance comparable to the column based method (Red C). Marker = Norgen’s HighRanger DNA Ladder./p> Figure 4. High DNA Quality. DNA was isolated from a number of plant species using Norgen's Plant DNA Isolation Kit (Magnetic Bead System) and Norgen's Plant/Fungi DNA Isolation Kit (column format, Cat. 26200). The purified DNA was then compared for DNA quality (A260/280). All DNA elutions isolated using Norgen’s Plant DNA Isolation Kit (Magnetic Bead System) showed an excellent comparable DNA quality to Norgen’s Plant/Fungi DNA Isolation Kit (column based, Cat. 26200), indicating the consistent and robust performance of the Plant DNA Isolation Kit (Magnetic Bead System). Figure 5. High DNA quality was confirmed by Real-time PCR. DNA was isolated from six different plant species using Norgen's Plan DNA Isolation Kit (Magnetic Bead System) and Norgen's Plant/Fungi DNA Isolation Kit (column format, Cat. 26200). To assess the quality of the purified DNA, 8 µL of the elution (total PCR reaction volume was 20 µL) was used in a real-time PCR reaction. As it can be seen, 18s rDNA amplification was successful in all cases without PCR inhibition, indicating the excellent quality of DNA purified using Norgen’s Plant DNA Isolation Kit (Magnetic Bead System). Figure 6. DNA was Isolated From Plant Tissue Using Norgen’s Plant DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL from 75 µL of elution were run on 1X TAE 1.2% agarose gel. Excellent DNA integrity, consistency, and yield were observed from the Plant DNA Isolation 96-Well Kit (Magnetic Bead System). Marker = Norgen’s HighRanger DNA Ladder (Cat. 11900). Figure 7. High DNA Quality Confirmed By Real-time PCR. DNA was isolated from plant tissue species using Norgen's Plant DNA Isolation 96-Well Kit (Magnetic Bead System). To assess the quality of the purified DNA, 2 µL of the elution (total PCR reaction volume was 20 µL) was used in a real-time PCR reaction. As it can be seen, 18s rDNA amplification was successful in all cases without PCR inhibition, indicating the excellent quality of DNA purified using Norgen’s Plant DNA Isolation 96-Well Kit (Magnetic Bead System). Next Click for expanded view Kit Specifications - Spin Column Column Binding Capacity 50 μg Maximum Column Loading Volume 650 μL Maximum Amount of Starting Material: Plant Tissues Fungi (wet weight) 100 mg 100 mg Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) 18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg Time to Complete 10 Purifications 45 minutes * Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage. * Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage. Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment. Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits Plants Plants (Cont'd) Fungi Tomato Turnip Aspergillus niger Grape Chinese Cabbage Mucor racemosus Peach Radish Cladosporium cladosporioides Plum Komatsuna Fusarium oxysporum Pine Needles Apricot Penicillium sp. Raspberry Sweet Potato Botrytis cinerea (Botryotinia fuckeliana) Strawberry Hydrangea Pichia sp. Legumes Fig Rhizopus oryzae Prosopis cineraria (Ghaf) Turf Alternaria tenuissima Sorghum grass Cherry Fusarium sp. Tobacco Saintpaulia Arabidopsis Lotus Lichen Carrot Corn seed Hansen Sunflower seed Pistachio Olive seed Soybean seed Component Cat. 26200 (50 preps) Cat. 26250 (250 preps) Cat. 26900 (192 preps) Cat. 58200 (50 preps) Cat. 62400 (192 preps) Lysis Buffer L 30 mL 1 x 30 mL2 x 60 mL 2 x 60 mL 60 mL 2 x 60 mL Binding Buffer I 7 mL 1 x 7 mL1 x 25 mL 25 mL 7 mL 25 mL Solution WN 18 mL 1 x 18 mL1 x 55 mL 55 mL 18 mL 55 mL Wash Solution A 38 mL 2 x 38 mL 2 x 38 mL - - Elution Buffer B 15 mL 30 mL 30 mL 8 mL 30 mL RNAse A 1 vial (80 μL) 5 vials (5 x 80 μL) 1 vial 1 vial 1 vial Magnetic Bead Suspension - - - 4 x 1.1 mL 2 x 8.5 mL Filter Columns 50 250 - - - Spin Columns 50 250 - - - Collection Tubes 100 500 - - - 96-Well Plate - - 2 - 2 96-Well Collection Plate - - 2 - - Adhesive Tape - - 4 - 2 Elution Tubes (1.7 mL) 50 250 - 50 - 96-Well Elution Plate - - 2 - 2 Product Insert 1 1 1 1 1 Documentation PROTOCOLS (26200) Plant/Fungi DNA Isolation Kit (Spin Column) - Protocol (50 Preps) (26250) Plant/Fungi DNA Isolation Kit (Spin Column) - Protocol (250 Preps) (26900) Plant/Fungi DNA Isolation 96 Well Kit (High Throughput) - Protocol (96-well) (58200) Plant DNA Isolation Kit (Magnetic Bead System) - Protocol (50 preps) (62400) Plant DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) - Protocol (2 x 96-well) APPLICATION NOTES Detection of Genomic DNA from Processed Plant Oils and Wheat Flour Detection of Genomic DNA from Seeds Optimizing Bead Homogenization of Plant Tissues for DNA and RNA Isolation Sensitivity and Specificity of Norgen’s HLVd TaqMan RT-PCR Kit.pdf SAFETY DATA SHEETS 26200, 26250, 26900, 58200, 62400 - Safety Data Sheet 58200, 62400 - Safety Data Sheet POSTERS Plant/Fungi DNA Isolation Kit Improving The Quality and Quantity of Total Genomic DNA Isolated From Difficult Plant and Soil Sam A Novel Technology to Extract Nucleic Acids from Different Environmental Samples including Plants, Water and Soil Compatibility of DNA and RNA Extraction Methods for Challenging Plant Species FLYERS Flyer High Throughput Plant DNA Isolation Kit Related Products HighRanger 1 kb DNA Ladder Plant/Fungi Total RNA Purification Kit Plant RNA/DNA Purification Kit
Plant/Fungi DNA Isolation Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plant/Fungi DNA Isolation Kits SKU 26200 Format Spin Column High Throughput Magnetic Bead System High Throughput Magnetic Bead System Size 50 Preps 250 Preps Quantity Trouver un distributeur Contact