Bacterial Genomic DNA Isolation Kit
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For research use only and NOT intended for in vitro diagnostics.
Aperçu du produit
This kit is designed for the rapid spin column preparation of genomic DNA from 2 x 109 viable bacterial cells (between 0.5 and 1.0 mL of culture). This kit can be used for both Gram-negative and Gram-positive bacteria including Escherichia coli and Bacillus cereus. Purified genomic DNA is of an excellent quality and yield, and is fully compatible with restriction enzyme digestions, sequencing, PCR, qPCR and more. Also available in 96-well format for high throughput applications.
Détails
Supporting Data
Figure 1. Isolation of Genomic DNA from both Gram Positive and Gram Negative Bacteria. The Bacterial Genomic DNA Isolation Kit was used to isolate genomic DNA from 1 mL overnight culture (1 x 109 cells) of the Gram negative bacteria E. coli (Lanes 1 and 2), the lysozyme-resistant Gram positive bacteria B. cereus (Lanes 3 and 4) and the Gram positive bacteria M. luteus (Lanes 5 and 6). Lane M is Norgen's UltraRanger 1kb DNA Ladder. For analysis 5 µL of the 200 µL eluted genomic DNA were loaded on a 1X TAE, 0.9% agarose gel.
Figure 2. High Yield Purification. The high yield of Norgen's Bacterial Genomic DNA Isolation Kit is illustrated by purifying genomic DNA from 1 mL overnight culture (1 x 109 cells) of both a Gram positive (B. cereus) and a Gram negative strain (E coli), and comparing the yield with two major competitors. The quantification of the DNA yield was performed by resolving 5 µL of the 200 µL of eluted DNA on a 1X TAE, 0.9% agarose gel followed by densitometry. With both types of bacteria, Norgen's kit was found to give a higher recovery than the competitor’s kits.
Kit Specifications (Spin Columns)
|
|
Input
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2 x 109 bacterial cells
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Column Binding Capacity
|
25 µg
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Average Yield*
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Up to 20 µg
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Time to Complete 10 Purifications |
1 hour
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* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
Component | Cat. 17900 (50 preps) | Cat. 17950 (192 preps) |
---|---|---|
Resuspension Solution A | 20 mL | 60 mL |
Lysis Buffer P | 18 mL | 60 mL |
Solution BX | 28 mL | 110 mL |
Wash Solution A | 18 mL | 2 x 38 mL |
Elution Buffer B | 30 mL | 2 x 30 mL |
Proteinase K | 12 mg | 50 mg |
Spin Columns | 50 | - |
96-Well Plate | - | 2 |
Adhesive Tape | - | 4 |
Collection Plate | - | 2 |
Collection Tubes | 50 | - |
Elution Tubes (1.7 mL) | 50 | - |
Elution Plate | - | 2 |
Product Insert | 1 | 1 |