Name: DNA Gel Extraction Kit
Brand: Norgen Biotek Corp.
SKU: 13100
Price: 105.000000 USD
Availability: InStock

Supporting Data

Figure 1. Efficient Recovery from Agarose Gels. The high yield of Norgen's DNA Gel Extraction Kit is illustrated by purification of 1.5 µg of a 500 bp fragment from a 1% agarose gel using Norgen's DNA Gel Extraction Kit and two other commercial kits. DNA recovery was quantified by running 300 ng (1/5th) of the input and 10 µL (1/5th) of the 50 µL DNA elution on a 1X TAE, 1% agarose gel followed by densitometry. The Norgen kit shows a significantly higher efficiency compared to the competitor kits based on the average of three purifications.

Figure 2. Efficient Recovery of Large DNA Fragments. The efficient recovery of Norgen's DNA Gel Extraction Kit is illustrated by purification of 1.5 µg of a 3,700 bp fragment from a 0.9% agarose gel using Norgen's DNA Gel Extraction Kit (Lanes 5-7) and a competitor's kit (Lanes 2-4). Eluted DNA was resolved on a 1X TAE, 0.9% agarose gel. Lane 1 indicates 300 ng (20%) of the input amount, while lanes 2-7 contain 10 µL (20%) of the 50 µL eluted amount. Norgen's kit shows a higher recovery than the competitors kit.

Kit Specifications
Column Binding Capacity	10 μg
Maximum Weight of Gel Slice	400 mg
Average Recovery	70-90%
Size of DNA Purified	100 - 15,000 bp
Minimum Elution Volume	30 μL
Time to Complete 10 Purifications	30 minutes

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 13100 (50 preps)
Binding Buffer G	80 mL
Wash Solution A	12 mL
Elution Buffer B	8 mL
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Documentation

PROTOCOLS

(13100) DNA Gel Extraction Kit - Protocol (50 prep)

SAFETY DATA SHEETS

DNA Gel Extraction Kit - SDS

FAQs

Spin Column

Why do I have poor DNA recovery?

Poor DNA recovery could be due to one of the following factors:

pH of the electrophoresis buffer was too high.
Ensure that a fresh running buffer is used for electrophoresis. When the buffer is re-used, it often exhibits increased pH and may subsequently reduce yields.
Gel slice was not completely melted in the Binding Buffer G.
The gel slice should be incubated at 55°C until completely dissolved. The slice should be vortexed every 2 to 3 minutes to assist dissolving.
Isopropanol was not added prior to binding.
Ensure that 1 gel volume of Isopropanol is added to the melted gel slice prior to binding to the column.
The appropriate amount of ethanol was not added to the Wash Solution A.
The Wash Solution A has been specifically designed to contain the appropriate amount of components. Ensure that the Wash Solution A was prepared with the correct amount of 96-100% ethanol.
Binding of DNA to the column was inefficient.
Binding of the DNA is dependent on both pH and salt concentration. Ensure that an appropriate amount of Binding Buffer G was used for the weight of the gel slice.
Binding Buffer G was not completely removed in the wash step.
Traces of salt left on the column from the binding step may interfere with the elution of the DNA. Ensure that the column is washed with the Wash Solution A.
Proper Elution Buffer B was not used.
The provided Elution Buffer B has been optimized for high elution recoveries. If water or TE buffer is used instead, ensure the pH is around 8.
Elution Buffer B was not placed directly onto the column bed.
It is important that the Elution Buffer B be placed directly onto the column bed, as this helps to increase recovery by ensuring an even passing of the buffer through the column. Do not pipette the Elution Buffer B onto the side of the column.

Why is the purified DNA not performing well in downstream applications?

Presence of impurities from the sample or the protocol can affect performance of DNA in downstream applications. This can happen when:

Removal of Wash Solution A is incomplete.
Ensure that the column is spun for 2 minutes during the wash step, in order to completely dry the column. Traces of Wash Solution A may remain in the eluted sample otherwise, and interfere with subsequent enzymatic reactions.
DNA was not washed with the provided Wash Solution A.
Traces of salt from the binding step may remain in the sample if the column is not washed with Wash Solution A. Salt may interfere with downstream applications, and thus must be washed from the column.

DNA Gel Extraction Kit