Supporting Data

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Figure 1.  Example of TaqMan One-step RT-PCR Positive result. PCR signals above the baseline from FAM, Tex615, Cy5, and HEX channel indicate the successful PCR. Individual amplification in any of the channels also indicates a positive result for the pathogen corresponding to that channel.

Figure 2.  Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM,TEX615, and Cy5 channels but amplification signal from HEX indicates the successful PCR.

Figure 3.  Example of TaqMan one-step RT-PCR inhibition result. No signal from all targets was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

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Channel Selection for Specified Platforms

HLVd	LCV	CanCV	Internal Control	Platform
FAM	Texas 615/ *Texas Red	Cy5	HEX	BioRad CFX96 Touch™ Real-Time PCR Detection System
FAM	JUN/*ROX	Cy5	VIC	QuantStudioTM
Green	Orange	Red	Yellow	Qiagen Rotor-Gene Q

Storage Conditions and Product Stability

All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM69950 (100 preps)	Cat. TM69910 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	-
HLVd/LCV/CanCV Primer & Probe Mix	280 μL	280 μL
HLVd/LCV/CanCV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Documentation

PROTOCOLS

(TM69950) HLVd/LCV/CanCV TaqMan RT-PCR Kit - Protocol (100 rxns)

APPLICATION NOTES

Sensitivity and Specificity of Norgen's HLVd TaqMan RT-PCR Kit

SAFETY DATA SHEETS

TM69950 - HLVD/LCV/CanCV TaqMan RT-PCR Detection Kit - SDS

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TM69910 - HLVd/LCV/CanCV TaqMan RT-PCR Detection Kit - SDS

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