Detergent-Free Total Protein Isolation Kit
For research use only and NOT intended for in vitro diagnostics.
Detergent-Free Total Protein Isolation Kit
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Supporting Data
Figure 1. Detergent-free Proteins Isolated from E. coli, Yeast, HeLa Cells and Kidney Tissue. Total proteins were isolated from 2 x 109 E. coli cells, 2 x 107 yeast cells, 1 million HeLa cells and 15 mg of Chinese hamster kidney tissue in duplicate using Norgen’s Detergent-Free Total Protein Isolation Kit. The total proteins were analyzed by running aliquots on 12% SDS-PAGE gels. Panel A corresponds to 10 µL of the 40 µL recovered proteins from E. coli., Panel B corresponds to 10 µL of the 40 µL recovered proteins from yeast, Panel C corresponds to 12 µL of the 60 µL recovered proteins from the HeLa cells, while Panel D corresponds to 2 µL of the 60 µL of recovered proteins from the kidney tissue. The proteins are intact and of the highest quality, and can be used in a number of different downstream applications.
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Kit Specifications
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| Maximum Amount Of Starting Material: Tissues Animal Cells Bacteria Yeast |
50 mg 1 x 107 cells 1 x 1010 cells 1 x 109 cells |
| Time to Process 12 Samples | Less than 10 minutes |
Storage Conditions and Product Stability
The Lysis Solution should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 30300 (50 preps) |
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| Lysis Solution | 4 mL |
| Lysis Tubes | 25 |
| Filter Columns | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |