EXTRAClean Exosomal RNA Isolation Kit
For the isolation of exosomal RNA from exosomes previously purified using Norgen's Exosome Purification Kits or Norgen’s EXTRAClean Exosome Purification Kits.
For research use only and NOT intended for in vitro diagnostics.
Powered by Bioz EXTRAClean Exosomal RNA Isolation Kit
For the isolation of exosomal RNA from exosomes previously purified using Norgen's Exosome Purification Kits or Norgen’s EXTRAClean Exosome Purification Kits.
Overview
- Isolates all sizes of exosomal and extracellular vesicle RNA, including microRNA.
- Bind and elute all RNA irrespective of size or GC content, without bias.
- No phenol extractions.
- No Proteinase K treatment.
- No carrier RNA.
- Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL.
- Purify high-quality RNA in 15-20 minutes.
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.
- Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.
The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Details
Supporting Data
Figure 1. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit.
Figure 2. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various plasma and serum using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit.
Figure 3. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from various urine volumes using EXTRAClean Urine Exosome Purification and RNA Isolation Maxi Kit. Calculations were relative to raw reads.
Figure 4. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various urine volumes using EXTRAClean Urine Exosome Purification and RNA Isolation Maxi Kit.
Figure 5. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various urine volumes using EXTRAClean Urine Exosome Purification and RNA Isolation Maxi Kit.
| Sample Type | Exosomes purified using Norgen's Purification Kits |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications | 35-40 minutes |
| Average Yields | Variable depending on specimen |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.