EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Kit
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For research use only and NOT intended for in vitro diagnostics.
EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Kit
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Supporting Data
Figure 1. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from plasma and serum samples using EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit. Calculations were relative to raw reads.
Figure 2. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various plasma and serum samples using EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit.
Figure 3. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various plasma and serum using EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit.
Figure 4. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from plasma and serum samples using EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit. Calculations were relative to raw reads.
Figure 5. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various plasma and serum samples using EXTRAClean Plasma/Serum Exosomeand Free-Circulating RNA Isolation Midi Kit.
Figure 6. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various plasma and serum using EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit.
Figure 7. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from plasma and serum samples using EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit. Calculations were relative to raw reads.
Figure 8. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various plasma and serum samples using EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit.
Figure 9. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various plasma and serum using EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit.
Kit Specifications (Spin Column)
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Minimum Plasma Input | 50 uL |
Maximum Plasma Input | 1 mL |
Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
Elution Volume | 50-100 μL |
Time to Complete 10 Purifications | 35-40 minutes |
Average Yields | Variable depending on specimen |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Documentation
(73410) - EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit (Spin Column) - Protocol (25 Preps)
(73420) - EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit (Spin Column) - Protocol (15 Preps)
(73410) - EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit (Spin Column) - Short Protocol (25 Preps)
(73420) - EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit (Spin Column) - Short Protocol (15 Preps)