FFPE RNA/DNA Purification Plus Kit For research use only and NOT intended for in vitro diagnostics. Powered by Bioz FFPE RNA/DNA Purification Plus Kit SKU 54300 Format Plus Size 50 Preps Quantity Trouver un distributeur Contact Overview Details Documentation Citations Aperçu du produit Norgen’s FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. The RNA and DNA are sequentially purified into separate fractions using this kit. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis. Détails Supporting Data Previous Figure 1. Superior Recovery of High Quality RNA and DNA from FFPE Spleen Tissues. Norgen's FFPE RNA/DNA Purification Plus Kit isolates FFPE RNA and DNA that exceeds the yield of competitors. Total RNA and DNA was isolated from equal amount of hamster FFPE spleen sections (20 micron thickness) using Norgen's FFPE RNA /DNA Purification Plus Kit and a leading competitor’s kits. Triplicate isolations were performed for each product. The top graphs demonstrate the mean yield of RNA (Panel A) and DNA (Panel B) according to NanoDrop measurement. The bottom graphs showed the mean 260:280 ratio and 260:230 ratio of RNA (Panel C) and DNA (Panel D) according to NanoDrop measurement. Norgen's kit consistently purified total RNA and DNA with a higher yield and higher quality than for those obtained using the market competitor's kits. Figure 2. Recovery of High Quality DNA and True Total RNA including microRNA from Hamster Spleen. Genomic DNA isolated using Norgen's FFPE RNA/DNA Purification Plus Kit is of high quality with effective qPCR amplification. Panel A showed that in a qPCR using primers against the 5S rRNA gene using equal amount of FFPE DNA as template, the DNA isolated with Norgen's kit showed better amplification (lower Ct value). Moreover, Norgen's FFPE RNA/DNA Purification Plus Kit isolates true total RNA including microRNA. With the same amount of RNA as template in RT-qPCR reactions for transcripts of miR-21 (Panel B for microRNA) and beta-Actin (Panel C for mRNA), RNA isolated by Norgen showed better amplification in beta-Actin and significantly better in miR-21 microRNA. Next Click for expanded view Kit Specifications Maximum Binding Capacity Up to 35 μg RNA Up to 10 μg DNA Maximum Column Loading Volume 600 μL Size of RNA Purified All sizes, including small RNA (< 200 nt) Maximum Amount of Starting Material 4 sections <20 μM thick 10 mg of unsectioned block Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year after the date of shipment. ComponentCat. 54300 (50 preps)Digestion Buffer A2 x 25 mLBuffer RL40 mLEnzyme Incubation Buffer A6 mLWash Solution A2 x 38 mLElution Solution A6 mLElution Buffer F15 mLProteinase K2 x 12 mgDNase I1 vialRNA Purification Micro Columns50DNA Purification Micro Columns50Collection Tubes100Elution Tubes (1.7 mL)100Product Insert1 Documentation PROTOCOLS (54300) FFPE RNA/DNA Purification Plus Kit - Protocol (50 prep) SAFETY DATA SHEETS (54300) FFPE RNA/DNA Purification Plus Kit - Safety Data Sheet FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Related Services NGS RNA Isolation Related Products FFPE RNA/DNA Purification Plus Kit FFPE RNA Purification 96-Well Kit FFPE DNA Purification Kit RNA/DNA/Protein Purification Plus Kit Leukocyte RNA Purification Kit
FFPE RNA/DNA Purification Plus Kit For research use only and NOT intended for in vitro diagnostics. Powered by Bioz FFPE RNA/DNA Purification Plus Kit SKU 54300 Format Plus Size 50 Preps Quantity Trouver un distributeur Contact