100 b RNA Ladder

Cat. 15002

100 b RNA Ladder

Cat.

For precise sizing of RNA molecules using native or denaturing gels

For research use only and NOT intended for in vitro diagnostics.

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100b RNA Ladder
(Cat. 15002) 50 loads

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Size:50 loads

Overview

100 b RNA Ladder

Eight discrete bands, ranging from 100 to 1,000 bases
Higher intensity band at 500 bases for easy reference
No need for staining or destaining as loading dye contains ethidium bromide
Convenient lyophilized format provides better product stability

The Norgen 100 b RNA Ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of small RNA molecules using native or denaturing agarose gels, and can be easily visualized under UV.

Contents

2 vials of lyophilized RNA ladder (25 loads each)
1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
2x loading buffer

Details

Supporting Data

Figure 1: 100 b RNA Ladder

Kit Specifications

Cat.

Specifications:

Eight discrete bands, ranging from 100 to 1,000 bases
Higher intensity band at 500 bases for easy reference

Contents:

2 vials of lyophilized RNA ladder (25 loads each)
1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
2x loading buffer

Instructions:

To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently.
Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1.5-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. There is no need for staining and destaining denaturing gels since Norgen’s loading buffer contains ethidium bromide.

Storage:

Store at -20°C.

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100 b RNA Ladder (Cat. 15002)

100 b RNA Ladder

100 b RNA Ladder

Size:50 loads

Overview

100 b RNA Ladder

Details

Supporting Data

Kit Specifications

Documentation

Citations