100 b RNA Ladder For precise sizing of RNA molecules using native or denaturing gels For research use only and NOT intended for in vitro diagnostics. Powered by Bioz 100 b RNA Ladder For precise sizing of RNA molecules using native or denaturing gels Price USD$109.00 SKU 15002 Size 50 Loads Quantity Overview Details Documentation Citations Overview Eight discrete bands, ranging from 100 to 1,000 basesHigher intensity band at 500 bases for easy referenceNo need for staining or destaining as loading dye contains ethidium bromideConvenient lyophilized format provides better product stability The Norgen 100 b RNA Ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of small RNA molecules using native or denaturing agarose gels, and can be easily visualized under UV.Contents2 vials of lyophilized RNA ladder (25 loads each)1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution2x loading buffer Details Supporting Data Previous Figure 1: 100 b RNA Ladder Next Click for expanded view Specifications: Eight discrete bands, ranging from 100 to 1,000 bases Higher intensity band at 500 bases for easy reference Contents: 2 vials of lyophilized RNA ladder (25 loads each) 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution 2x loading buffer Instructions: To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently. Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1.5-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. There is no need for staining and destaining denaturing gels since Norgen’s loading buffer contains ethidium bromide. Storage: Store at -20°C. Documentation PROTOCOLS 15002 - Product Insert SAFETY DATA SHEETS 100 b RNA Ladder - MSDS Citations Title Duplex reverse transcription-polymerase chain reaction system to detect Potato spindle tuber viroid using an internal control mRNA and a non-infectious positive control RNA Journal Journal of General Plant Pathology. 2009. Authors Hataya T.
100 b RNA Ladder For precise sizing of RNA molecules using native or denaturing gels For research use only and NOT intended for in vitro diagnostics. Powered by Bioz 100 b RNA Ladder For precise sizing of RNA molecules using native or denaturing gels Price USD$109.00 SKU 15002 Size 50 Loads Quantity
