A ready-to-use solution optimized for robust amplification of high yield products

  • Convenient ready-to-use solution
  • High sensitivity and yield
  • Robust amplification

ItemsCat. #Size
2X PCR Master Mix 28007 100 reactions

2X PCR Master Mix

Norgen’s 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction. This convenient 2X PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of DNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Reagents Supplied - Cat # 28007

  • 2X PCR Master Mix (1 Vial, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions

Storage Conditions and Product Stability
Norgen’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

Title Handbook On Cassava: Status Of Cassava Processing And Challenges In The Coastal, Eastern And Western Regions Of Kenya
Journal Nova Science Publishers, Inc.. 2017
Authors C. M. Githunguri, M. Gatheru and S. M. Ragwa
Title Molecular Detection of Virulence Genes and Antibiotic Resistance Patterns of Escherichia coli O157:H7 Isolated from Raw Beef Sold in Abeokuta, South-West Nigeria
Journal Nigerian Journal of Biotechnology. 2016.
Authors Oloyede, A. R., Afolabi, O. R., & Olalowo, O. S
Title Identification Of Cuscuta Campestrisyuncker In Uae: Study Of Bar Code Loci-Rbcl, Matk And Trnh-Psbain The Uae And Egyptian Cultivars And In The Respective Host Plants Basil And Jute
Journal International Journal of Research in Science. 2016.
Authors Alsaadi, A., Saif, M., Yasser, M., Gaur, S., El-wakil, H., Maloukh, L., & Lakshmi, T. V. R
Title Production of Lipases in Solid-State Fermentation by Aspergillus Niger F7-02 with Agricultural Residues
Journal Journal of Microbiology, Biotechnology and Food Sciences. 2015.
Authors Olayinka Quadri Adio, Sarafdeen Olateju Kareem, Michael Bamitale Osho, Adebukola Mobolaji Omemu
Title Molecular Epidemiology of Escherichia Coli in HIV-Positive Individuals in South west Nigeria
Journal International Archives of Medicine. 2015.
Authors Lawrence Ehis Okoror, Duna Christina Fashina, Titilayo Silifat Jimoh, Ezekiel Ayo Oisagah
Title Na+/H+ Exchanger Isoform 1 Induced Cardiomyocyte Hypertrophy Involves Activation of p90 Ribosomal S6 Kinase
Journal PLOS ONE. 2015.
Authors Maiy Jaballah, Iman A. Mohamed, Bayan Alemrayat, Fatima Al-Sulaiti, Mohamed Mlih, Fatima Mraiche
Title TNF (-308) Polymorphism has no Influence on the Clinical Severity of Sickle Cell Anemia in Saudi Patients.
Journal Journal of Applied Hematology. 2013.
Authors Hamad Z, Aljedai A, Halwani R, Alsultan A.
Title Microbial contamination of human milk purchased via the Internet
Journal Pediatrics. 2013.
Authors Keim SA, Hogan JS, McNamara KA, Gudimetla V, Dillon CE, Kwiek JJ, Geraghty SR.
Title Characterization of unstable microsatellites in mice: No evidence for germline mutation induction following gamma-radiation exposure.
Journal Environmental and Molecular Mutagenesis. 2012.
Authors Beal MA, Glenn TC, Lance SL, Somers CM.