Overview

Norgen’s 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl₂, KCl and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction. This convenient 2X PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of DNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Details

Supporting Data

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Figure 1. Successful Amplification of Different Sized Amplicons. Norgen’s 2X PCR Master Mix was used in a series of PCR reactions to amplify a number of different sized amplicons from the adenovirus E2 region. Lanes 1 and 2 correspond to a 189 bp product, Lanes 3 and 4 correspond to a 565 bp product, Lanes 5 and 6 correspond to a 1501 bp product, and Lanes 7 and 8 correspond to a 2202 bp product. Norgen’s 2X PCR Master Mix was able to amplify all the different sized products with a high sensitivity and yield. Lane M1 is Norgen’s PCR Sizer 100 bp DNA Ladder (Cat #11400) and Lane M2 is Norgen’s MidRanger 1kb DNA Ladder (Cat # 11700).

 

Figure 2. Highly Stable after Multiple Freeze-Thaw Cycles  The stability of Norgen’s 2X PCR Master Mix was tested by subjected the mix to multiple freezing and thawing cycles, followed by using the Master Mix to amplify the 16s rDNA (1.5 kb) of Staphylococcus aureus gDNA. Norgen’s 2X PCR Master Mix showed robustness and outstanding stability from multiple freezing and thawing steps at -20ºC. M: FastRunner DNA Ladder (Cat#12800). Ten μL of each PCR reaction was loaded on a 1% TAE agarose gel.

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