• AAV Purification from any input – cell fraction or media fraction
  • Purification from 500 mL of cell culture supernatant or from ~15 mL of cell pellet per kit
  • Rapid purification within 2 to 4.5 hours
  • High AAV recovery, up to 90%
  • No specialized equipment needed
  • Purification from a variety of AAV serotypes (including AAV6 and AAV9)
  • Purify AAV cell culture supernatant from 1 mL to 33.5 mL input per prep
  • Purify AAV cell pellet from 1 mL of input per prep
  • Yields highly active AAV for in vivo and in vitro experiments
  • Up to 33X sample concentration

ItemsCat. #Size
AAV Purification Kit 66100 15 preps

AAV Purification Kit

Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Kit provides a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on precipitation onto Norgen Biotek’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro and in vivo transduction experiments.

Norgen’s AAV Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant (SN) or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit.

Kit Specifications
Resin Binding Capacity (total per kit) At least 5 x 1010 AAV particles as determined by qPCR
AAV Vector Serotype AAV6, AAV9 and others
Input Type Cells, media
Input Volume (AAV supernatant) 1 - 33.5 mL SN per prep
(500 mL SN in total)
Input Volume (AAV cell pellet) 1 mL cell pellet per prep
(15 mL in total)
Minimum Elution Volume 2.5 to 4.5 hours with 1 hour hands on time
In vivo transduction Yes

Storage Conditions and Product Stability

HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C . All the reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 66100 (15 preps)
Lysis Buffer S 5.5 mL
HL-SAN Nuclease 102 µL
Binding Buffer A 4 mL
Purification Solution C 60 mL
Purification Solution D 130 mL
Wash Solution C 2 x 130 mL
Slurry E 2 x 12.5 mL
Elution Buffer O (store at 4°C) 66 mL
Protein Neutralizer 4 mL
Elution tubes (1.7 mL) 50
Product Insert 1