|AAV Purification Kit||66100||15 preps|
AAV Purification Kit
Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Kit provides a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on precipitation onto Norgen Biotek’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro and in vivo transduction experiments.
Norgen’s AAV Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant (SN) or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit.
|Resin Binding Capacity (total per kit)||At least 5 x 1010 AAV particles as determined by qPCR|
|AAV Vector Serotype||AAV6, AAV9 and others|
|Input Type||Cells, media|
|Input Volume (AAV supernatant)||1 - 33.5 mL SN per prep
(500 mL SN in total)
|Input Volume (AAV cell pellet)||1 mL cell pellet per prep
(15 mL in total)
|Time to Complete Purifications||2.5 to 4.5 hours with 1 hour hands on time|
|In vivo transduction||Yes|
Storage Conditions and Product Stability
HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C . All the reagents should remain stable for at least 1 year in their unopened containers.
Copyright © 2019 - Norgen Biotek Corp.