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For concentrating and purifying AAV vectors from cell lysate and cell culture media
For research use only and NOT intended for in vitro diagnostics.
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Kit Specifications
Kit Specifications
|
|
Resin Binding Capacity (total per kit) | At least 5 x 1010 AAV particles as determined by qPCR |
AAV Vector Serotype | AAV6, AAV9 and others |
Input Type | Cells, media |
Input Volume (AAV supernatant) | 1 - 33.5 mL SN per prep (500 mL SN in total) |
Input Volume (AAV cell pellet) | 1 mL cell pellet per prep (15 mL in total) |
Time to Complete Purifications | 2.5 to 4.5 hours with 1 hour hands on time |
In vivo transduction | Yes |
Storage Conditions and Product Stability
HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
Kit Specifications
|
|
Column Binding Capacity | At least 5 x 109 AAV particles as determined by qPCR |
AAV Vector Serotype | Any |
Average Recovery | > 80% |
Input Type | Cells, media, or mixed |
Input Volume
|
0.5 mL - 8 mL
|
Minimum Elution Volume |
200 µL
|
Time to Complete Purifications |
1 - 2 hours
|
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.
Kit Specifications
|
|
Column Binding Capacity | At least 1 x 1010 AAV particles as determined by qPCR |
AAV Vector Serotype | Any |
Average Recovery | > 80% |
Input Type | Cells, media, or mixed |
Input Volume
|
8 mL - 45 mL
|
Minimum Elution Volume |
1 mL
|
Time to Complete 4 Purifications |
2 - 2.5 hours
|
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.
Kit Specifications
|
|
Column Binding Capacity | At least 1 x 1010 AAV particles as determined by qPCR |
AAV Vector Serotype | Any |
Average Recovery | 50 - 80% |
Input Type | Cells, media, or mixed |
Input Volume
|
90 mL - 900 mL
|
Minimum Elution Volume |
1 mL - 10 mL
|
Time to Complete Purifications |
2.5 - 3.5 hours
|
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.
Supporting Data
AAV Purification Kits
Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Kits provide fast and simple procedures for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on precipitation onto Norgen Biotek’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro and in vivo transduction experiments.
AAV Purification Kit
Norgen’s AAV Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant (SN) or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit. Up to 33X sample concentration.
AAV Purification Mini Kit
Each spin column is able to concentrate and purify AAV from 0.5-8 mL of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (200 µL). Preparation time for 4 samples is 1.5 hours, with 45 minutes of hands-on time.
AAV Purification Midi Kit
Each spin column is able to concentrate and purify AAV from 8 mL up to 45 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1 mL). The kit may be used to purify up to 8 x 25 mL or 4 x 45 mL of samples using the included columns. Preparation time for 4 samples is approximately 2 to 2.5 hours, with 1.5 hours of hands on time.
AAV Purification Maxi Kit (Slurry Format)
Each spin column is able to concentrate and purify AAV from 45 mL to 90 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 200X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1-10 mL) using the optional concentration step. The kit may be used to purify up to 1 x 900 mL samples or 10 x 45-90 mL samples using the included columns. Preparation time for 1 x 900 mL sample is approximately 2.5 to 3.5 hours, with an optional concentration step requiring an additional 30 min.
Supporting Data
Kit Specifications
Kit Specifications
|
|
Resin Binding Capacity (total per kit) | At least 5 x 1010 AAV particles as determined by qPCR |
AAV Vector Serotype | AAV6, AAV9 and others |
Input Type | Cells, media |
Input Volume (AAV supernatant) | 1 - 33.5 mL SN per prep (500 mL SN in total) |
Input Volume (AAV cell pellet) | 1 mL cell pellet per prep (15 mL in total) |
Time to Complete Purifications | 2.5 to 4.5 hours with 1 hour hands on time |
In vivo transduction | Yes |
Storage Conditions and Product Stability
HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
Kit Specifications
|
|
Column Binding Capacity | At least 5 x 109 AAV particles as determined by qPCR |
AAV Vector Serotype | Any |
Average Recovery | > 80% |
Input Type | Cells, media, or mixed |
Input Volume
|
0.5 mL - 8 mL
|
Minimum Elution Volume |
200 µL
|
Time to Complete Purifications |
1 - 2 hours
|
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.
Kit Specifications
|
|
Column Binding Capacity | At least 1 x 1010 AAV particles as determined by qPCR |
AAV Vector Serotype | Any |
Average Recovery | > 80% |
Input Type | Cells, media, or mixed |
Input Volume
|
8 mL - 45 mL
|
Minimum Elution Volume |
1 mL
|
Time to Complete 4 Purifications |
2 - 2.5 hours
|
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.
Kit Specifications
|
|
Column Binding Capacity | At least 1 x 1010 AAV particles as determined by qPCR |
AAV Vector Serotype | Any |
Average Recovery | 50 - 80% |
Input Type | Cells, media, or mixed |
Input Volume
|
90 mL - 900 mL
|
Minimum Elution Volume |
1 mL - 10 mL
|
Time to Complete Purifications |
2.5 - 3.5 hours
|
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.