AAV Purification Kits

Cat. 66100, 63200, 63300, 63250
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AAV Purification Kits

Cat. 66100, 63200, 63300, 63250

For concentrating and purifying AAV vectors from cell lysate and cell culture media

  • AAV Purification from any input – cell fraction or media fraction
  • High AAV recovery, up to 90%
  • No specialized equipment needed
  • Purification from a variety of AAV serotypes (including AAV6 and AAV9)
  • Yields highly active AAV for in vivo and in vitro experiments
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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AAV Purification Kit
(Cat. 66100)
15 preps
AAV Purification Mini Kit
(Cat. 63200)
20 preps
AAV Purification Midi Kit
(Cat. 63300)
4-8 preps
AAV Purification Maxi Slurry Kit
(Cat. 63250)
1-10 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • AAV Purification from any input – cell fraction or media fraction
  • High AAV recovery, up to 90%
  • No specialized equipment needed
  • Purification from a variety of AAV serotypes (including AAV6 and AAV9)
  • Yields highly active AAV for in vivo and in vitro experiments
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

AAV Purification Kits

Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Kits provide fast and simple procedures for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on precipitation onto Norgen Biotek’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro and in vivo transduction experiments.

AAV Purification Kit

Norgen’s AAV Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant (SN) or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit. Up to 33X sample concentration.

AAV Purification Mini Kit

Each spin column is able to concentrate and purify AAV from 0.5-8 mL of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (200 µL). Preparation time for 4 samples is 1.5 hours, with 45 minutes of hands-on time.

AAV Purification Midi Kit

Each spin column is able to concentrate and purify AAV from 8 mL up to 45 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1 mL). The kit may be used to purify up to 8 x 25 mL or 4 x 45 mL of samples using the included columns. Preparation time for 4 samples is approximately 2 to 2.5 hours, with 1.5 hours of hands on time.

AAV Purification Maxi Kit (Slurry Format)

Each spin column is able to concentrate and purify AAV from 45 mL to 90 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 200X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1-10 mL) using the optional concentration step. The kit may be used to purify up to 1 x 900 mL samples or 10 x 45-90 mL samples using the included columns. Preparation time for 1 x 900 mL sample is approximately 2.5 to 3.5 hours, with an optional concentration step requiring an additional 30 min.

Kit Specifications

You have selected: Cat. 66100
Kit Specifications
Resin Binding Capacity (total per kit) At least 5 x 1010 AAV particles as determined by qPCR
AAV Vector Serotype AAV6, AAV9 and others
Input Type Cells, media
Input Volume (AAV supernatant) 1 - 33.5 mL SN per prep
(500 mL SN in total)
Input Volume (AAV cell pellet) 1 mL cell pellet per prep
(15 mL in total)
Time to Complete Purifications 2.5 to 4.5 hours with 1 hour hands on time
In vivo transduction Yes

 

Storage Conditions and Product Stability

HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 63200
Kit Specifications
Column Binding Capacity At least 5 x 109 AAV particles as determined by qPCR
AAV Vector Serotype Any
Average Recovery > 80%
Input Type Cells, media, or mixed
Input Volume
0.5 mL - 8 mL
Minimum Elution Volume
200 µL
Time to Complete Purifications
1 - 2 hours

 
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 63300
Kit Specifications
Column Binding Capacity At least 1 x 1010 AAV particles as determined by qPCR
AAV Vector Serotype Any
Average Recovery > 80%
Input Type Cells, media, or mixed
Input Volume
8 mL - 45 mL
Minimum Elution Volume
1 mL
Time to Complete 4 Purifications
2 - 2.5 hours

 
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 63250
Kit Specifications
Column Binding Capacity At least 1 x 1010 AAV particles as determined by qPCR
AAV Vector Serotype Any
Average Recovery 50 - 80%
Input Type Cells, media, or mixed
Input Volume
90 mL - 900 mL
Minimum Elution Volume
1 mL - 10 mL
Time to Complete Purifications
2.5 - 3.5 hours

 
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 66100 (15 preps) Cat. 63200 (20 preps) Cat. 63300 (4-8 preps) Cat. 63250 (1-10 preps)
Lysis Buffer S 5.5 mL 5.5 mL 5.5 mL 20 mL
DNAse I - 2 x 25 uL 2 x 25 uL 210 μL
RNAse A - 60 μL 60 μL 240 μL
HL-SAN Nuclease 102 μL - - -
Binding Buffer A 20 mL 4 mL 4 mL 2 x 8 mL
Purification Solution C 60 mL - - -
Purification Solution D 130 mL - - -
Wash Solution C 2 x 130 mL 60 mL 60 mL 3 x 60 mL
Slurry E 12.5 mL - - 2 x 14.5 mL
Elution Buffer O 66 mL 8.5 mL 8.5 mL 66 mL
Protein Neutralizer 4 mL 4 mL 4 mL 4 mL
Spin Columns - 20 - -
Mini Spin Columns - 20 - -
Midi Spin Columns (grey contents) with Collection Tubes - - 8 10
Midi Spin Columns (white contents) with Collection Tubes - - 8 -
Maxi Spin Columns (grey contents) with Collection Tubes - - - 10
Maxi Spin Columns (white contents) with Collection Tubes - - - 10
Collection Tubes - 40 - -
Elution tubes (1.7 mL) 50 20 - -
Midi Elution tubes (15 mL) - - 8 10
Maxi Elution tubes (50 mL) - - - 10
Product Insert 1 1 1 1
Component
Lysis Buffer S
Wash Solution C
Binding Buffer A
Elution Buffer O (store at 4°C)
Protein Neutralizer
Spin Columns (White with Blue O-ring)
Mini Spin Columns (Grey) 20
Collection Tubes 40
Elution Tubes (1.7 mL) 20
Product Insert 1

AAV Purification Kits

Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Kits provide fast and simple procedures for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on precipitation onto Norgen Biotek’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro and in vivo transduction experiments.

AAV Purification Kit

Norgen’s AAV Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant (SN) or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit. Up to 33X sample concentration.

AAV Purification Mini Kit

Each spin column is able to concentrate and purify AAV from 0.5-8 mL of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (200 µL). Preparation time for 4 samples is 1.5 hours, with 45 minutes of hands-on time.

AAV Purification Midi Kit

Each spin column is able to concentrate and purify AAV from 8 mL up to 45 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1 mL). The kit may be used to purify up to 8 x 25 mL or 4 x 45 mL of samples using the included columns. Preparation time for 4 samples is approximately 2 to 2.5 hours, with 1.5 hours of hands on time.

AAV Purification Maxi Kit (Slurry Format)

Each spin column is able to concentrate and purify AAV from 45 mL to 90 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 200X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1-10 mL) using the optional concentration step. The kit may be used to purify up to 1 x 900 mL samples or 10 x 45-90 mL samples using the included columns. Preparation time for 1 x 900 mL sample is approximately 2.5 to 3.5 hours, with an optional concentration step requiring an additional 30 min.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 66100
Kit Specifications
Resin Binding Capacity (total per kit) At least 5 x 1010 AAV particles as determined by qPCR
AAV Vector Serotype AAV6, AAV9 and others
Input Type Cells, media
Input Volume (AAV supernatant) 1 - 33.5 mL SN per prep
(500 mL SN in total)
Input Volume (AAV cell pellet) 1 mL cell pellet per prep
(15 mL in total)
Time to Complete Purifications 2.5 to 4.5 hours with 1 hour hands on time
In vivo transduction Yes

 

Storage Conditions and Product Stability

HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 63200
Kit Specifications
Column Binding Capacity At least 5 x 109 AAV particles as determined by qPCR
AAV Vector Serotype Any
Average Recovery > 80%
Input Type Cells, media, or mixed
Input Volume
0.5 mL - 8 mL
Minimum Elution Volume
200 µL
Time to Complete Purifications
1 - 2 hours

 
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 63300
Kit Specifications
Column Binding Capacity At least 1 x 1010 AAV particles as determined by qPCR
AAV Vector Serotype Any
Average Recovery > 80%
Input Type Cells, media, or mixed
Input Volume
8 mL - 45 mL
Minimum Elution Volume
1 mL
Time to Complete 4 Purifications
2 - 2.5 hours

 
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 63250
Kit Specifications
Column Binding Capacity At least 1 x 1010 AAV particles as determined by qPCR
AAV Vector Serotype Any
Average Recovery 50 - 80%
Input Type Cells, media, or mixed
Input Volume
90 mL - 900 mL
Minimum Elution Volume
1 mL - 10 mL
Time to Complete Purifications
2.5 - 3.5 hours

 
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.

Component Cat. 66100 (15 preps) Cat. 63200 (20 preps) Cat. 63300 (4-8 preps) Cat. 63250 (1-10 preps)
Lysis Buffer S 5.5 mL 5.5 mL 5.5 mL 20 mL
DNAse I - 2 x 25 uL 2 x 25 uL 210 μL
RNAse A - 60 μL 60 μL 240 μL
HL-SAN Nuclease 102 μL - - -
Binding Buffer A 20 mL 4 mL 4 mL 2 x 8 mL
Purification Solution C 60 mL - - -
Purification Solution D 130 mL - - -
Wash Solution C 2 x 130 mL 60 mL 60 mL 3 x 60 mL
Slurry E 12.5 mL - - 2 x 14.5 mL
Elution Buffer O 66 mL 8.5 mL 8.5 mL 66 mL
Protein Neutralizer 4 mL 4 mL 4 mL 4 mL
Spin Columns - 20 - -
Mini Spin Columns - 20 - -
Midi Spin Columns (grey contents) with Collection Tubes - - 8 10
Midi Spin Columns (white contents) with Collection Tubes - - 8 -
Maxi Spin Columns (grey contents) with Collection Tubes - - - 10
Maxi Spin Columns (white contents) with Collection Tubes - - - 10
Collection Tubes - 40 - -
Elution tubes (1.7 mL) 50 20 - -
Midi Elution tubes (15 mL) - - 8 10
Maxi Elution tubes (50 mL) - - - 10
Product Insert 1 1 1 1
Component
Lysis Buffer S
Wash Solution C
Binding Buffer A
Elution Buffer O (store at 4°C)
Protein Neutralizer
Spin Columns (White with Blue O-ring)
Mini Spin Columns (Grey) 20
Collection Tubes 40
Elution Tubes (1.7 mL) 20
Product Insert 1
 


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