AAV Purification Maxi Slurry Kit (Cat. 63250)

AAV Purification from any input – cell fraction, media fraction, or mixed cells and media
Rapid purification within 2.5 to 3.5 hours, with optional concentration step
1-10 mL final elution volume
High AAV recovery, up to 90%
No specialized equipment needed
Purification from a variety of AAV serotypes
Purify AAV from 90 mL to 900 mL of input per run
Combine 2 kits for 1,800 mL input purification per run
Yields highly active AAV for in vitro experiments
Up to 200-fold sample concentration
Multiple purifications (10 per kit, 90 mL each) can be done in parallel, or 1 single large purification of 900 mL of input

Items	Cat. #	Size
AAV Purification Maxi Slurry Kit	63250	1-10 preps

AAV Purification Maxi Slurry Kit

Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV purification kits provide a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a precipitation and centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion and column purification. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1-10 mL) using the optional concentration step.

Kit Specifications
Column Binding Capacity	At least 1 x 10¹⁰ AAV particles as determined by qPCR
AAV Vector Serotype	Any
Average Recovery	50 - 80%
Input Type	Cells, media, or mixed
Input Volume	90 mL - 900 mL
Minimum Elution Volume	1 mL - 10 mL
Time to Complete Purifications	2.5 - 3.5 hours

Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. All the reagents should remain stable for at least 1 year in their unopened containers.