|AAV Purification Maxi Slurry Kit||63250||1-10 preps|
AAV Purification Maxi Slurry Kit
Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV purification kits provide a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a precipitation and centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion and column purification. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1-10 mL) using the optional concentration step.
|Column Binding Capacity||At least 1 x 1010 AAV particles as determined by qPCR|
|AAV Vector Serotype||Any|
|Average Recovery||50 - 80%|
|Input Type||Cells, media, or mixed|
90 mL - 900 mL
|Minimum Elution Volume||
1 mL - 10 mL
|Time to Complete Purifications||
2.5 - 3.5 hours
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. All the reagents should remain stable for at least 1 year in their unopened containers.
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