AAV Purification Mini Kit (Cat. 63200)

AAV Purification from any input – cell fraction, media fraction, or mixed cells and media
Rapid purification within 1 to 2 hours
High AAV recovery, up to 90%
No specialized equipment needed
Purification from a variety of AAV serotypes
Purify AAV from 0.5 mL to 8 mL input
Yields highly active AAV for in vitro experiments
Up to 50x sample concentration
Multiple purifications (20 per kit) can be done in parallel for rapid screening experiments

Items	Cat. #	Size
AAV Purification Mini Kit	63200	20 preps

AAV Purification Mini Kit

Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Mini Kit provides a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a precipitation and centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (200 µL).

Kit Specifications
Column Binding Capacity	At least 5 x 10⁹ AAV particles as determined by qPCR
AAV Vector Serotype	Any
Average Recovery	> 80%
Input Type	Cells, media, or mixed
Input Volume	0.5 mL - 8 mL
Minimum Elution Volume	200 µL
Time to Complete Purifications	1 - 2 hours

Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. All the reagents should remain stable for at least 1 year in their unopened containers.

Kit Components

Component	Cat. 63200 (20 preps)
Lysis Buffer S	5.5 mL
DNAse I (store at -20°C)	2 x 25 uL
RNAse A (store at -20°C)	60 uL
Wash Solution C	60 mL
Binding Buffer A	4 mL
Elution Buffer O (store at 4°C)	8.5 mL
Protein Neutralizer	4 mL
Spin Columns (White with Blue O-ring)	20
Mini Spin Columns (Grey)	20
Collection Tubes	40
Elution Tubes (1.7 mL)	20
Product Insert	1