AAV Quantification Kit

Overview

Norgen’s AAV Quantification Kit is designed for the detection of adeno-associated virus (AAV) inverted terminal repeat (ITR) sequences in a real-time PCR based on the use of TaqMan® technology. A purified standard based solely on the AAV2 ITR sequence purified using Norgen’s proprietary silicon carbide technology simplifies the generation of a reliable standard curve for AAV quantification. Avoidance of a plasmid based standard eliminates problems associated with efficient melting of the ITR sequence due to coupling of the ITR to the much longer plasmid sequence, as well as variability due to rearrangements/duplications/deletions of the recombination prone ITR. An easy and rapid method for viral DNA extraction simplifies the step of obtaining AAV DNA while simultaneously eliminating contaminating non-encapsidated DNA.  Norgen’s AAV Quantification Kit can facilitate pre-clinical studies that require accurate vector titration as well as interlab comparisons of vector quantities. This kit is designed for research use only and not for use in diagnostic procedures.

Details

Supporting Data

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Figure 1. Amplification of ten fold serial dilutions of purified Norgen AAV Standard: 10^-2 to 10^-8 dilutions and no target control (NTC). 

Figure 2. XY Scatter Plot of Ct versus log copy number demonstrates high linearity for AAV standard dilution series from 10^-2  dilution to 10^-8 dilution (copy number of 2,000 to 2,000,000,000).

Figure 3. A) Determination of copy number using Norgen's AAV Quantification Kit for samples purified with Norgen's AAV Purification Kit based on 1 mL, 3 mL, or 9 mL input volumes. B) Transduction of HTX cells with 100 µL of AAV preparation from 1 mL, 3 mL, or 9 mL input volumes purified using the Norgen AAV Purification Kit.

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