• Rapid AAV DNA extraction and quantification can be completed in 2 hours
  • Universal qPCR assay targeting the AAV2 ITR enables quantification of most AAV vector constructs
  • Purified DNA standard enables easy comparison of data between different lab groups
  • Non-plasmid based standard prevents quantification artifacts due to insufficient melting of plasmid sequences
  • DNAse digestion step aids in eliminating non-viral DNA
  • Minimal sample handling for maximum AAV DNA recovery

ItemsCat. #Size
AAV Quantification Kit 63800 24 reactions

AAV Quantification Kit

Norgen’s AAV Quantification Kit is designed for the detection of adeno-associated virus (AAV) inverted terminal repeat (ITR) sequences in a real-time PCR based on the use of TaqMan® technology. A purified standard based solely on the AAV2 ITR sequence purified using Norgen’s proprietary silicon carbide technology simplifies the generation of a reliable standard curve for AAV quantification. Avoidance of a plasmid based standard eliminates problems associated with efficient melting of the ITR sequence due to coupling of the ITR to the much longer plasmid sequence, as well as variability due to rearrangements/duplications/deletions of the recombination prone ITR. An easy and rapid method for viral DNA extraction simplifies the step of obtaining AAV DNA while simultaneously eliminating contaminating non-encapsidated DNA. Norgen’s AAV Quantification Kit can facilitate pre-clinical studies that require accurate vector titration as well as interlab comparisons of vector quantities. This kit is designed for research use only and not for use in diagnostic procedures.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.

Kit Components
Component Cat. 63800 (24 rxns)
Enzyme Incubation Buffer A 500 µL
DNase I 25 µL
DNase Stop Solution 100 µL
2X PCR Mastermix 350 µL
AAV Primer & Probe Mix 90 µL
AAV Standard 6 µL
Nuclease-Free Water (Negative Control) 1.25 mL
Product Insert 1