ProteoSpin™ Abundant Serum Protein Depletion Kit (Cat. 17300)
ProteoSpin™ Abundant Serum Protein Depletion Kit
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ProteoSpin™ Abundant Serum Protein Depletion Kit
For the rapid depletion of abundant proteins from serum samples
- Rapid and efficient removal of abundant proteins from serum and plasma samples
- Process 10 samples in 30 minutes
- Allows for visualization of low abundance proteins
- Convenient and affordable spin column protocol
- Generic protocol allows for depletion from human and other animal samples
- Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 17300) 25 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
| Maximum Column Capacity |
500 μg
|
| Minimum Column Capacity |
200 μg
|
| Minimum Elution Volume |
30 μL
|
| Time to Process 10 Samples |
30 minutes
|
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
Supporting Data
| Title | Clinical Proteomics: Liquid Chromatography–Mass Spectrometry (LC–MS) Purification Systems |
| Journal | Protein Chromatography. 2016. |
| Authors | Henry M, Meleady P. |
| Title | Neonatal NET-inhibitory factor and related peptides inhibit neutrophil extracellular trap formation |
| Journal | The Journal of Clinical Investigation. 2016. |
| Authors | Yost CC, Schwertz H, Cody MJ, Wallace JA, Campbell RA, Vieira-de-Abreu A, Araujo CV, Schubert S, Harris ES, Rowley JW, Rondina MT, Fulcher JM, Koening CL, Weyrich AS, Zimmerman GA |
| Title | Doublecortin-Like Kinase 1 Is Elevated Serologically in Pancreatic Ductal Adenocarcinoma and Widely Expressed on Circulating Tumor Cells |
| Journal | PLoS ONE. 2015. |
| Authors | Dongfeng Qu, Jeremy Johnson, Parthasarathy Chandrakesan, Nathaniel Weygant, Randal May, Nicole Aiello, Andrew Rhim, Lichao Zhao, Wei Zheng, Stanley Lightfoot, Shubham Pant, Jeremy Irvan, Russell Postier, James Hocker, Jay S. Hanas, Naushad Ali, Sripathi M. Sureban, Guangyu An, Michael J. Schlosser, Ben Stanger, Courtney W. Houchen |
| Title | Identification of plasma Complement C3 as a potential biomarker for neuroblastoma using a quantitative proteomic approach. |
| Journal | Journal of Proteomics. 2013. |
| Authors | Kim PY, Tan O, Diakiw S, Carter D, Sekerye EO, Wasinger VC, Liu T, Kavallaris M, Norris MD, Haber M, Chesler L, Dolnikov A, Trahair TN, Cheung NK, Marshall GM, Cheung BB. |
| Title | Quantitation of irbesartan and major proteins in human plasma by mass spectrometry with time-of-flight analyzer. |
| Journal | Journal of Pharmaceutical and Biomedical Analysis. 2011. |
| Authors | Lu CY, Feng CH. |
| Title | Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals. |
| Journal | Analytical Biochemistry. 2010. |
| Authors | Kanekoa H, Kamiieb J, Kawakamib H, Anadac T, Hondac Y, Shiraishid N, Kamakurae S, Terasakif T, Shimauchia H, Suzukic O. |
| Title | Modification of major plasma proteins by acrylamide and glycidamide: preliminary screening by nano liquid chromatography with tandem mass spectrometry. |
| Journal | Analytica Chimica Acta. 2010. |
| Authors | Chia-Hsien Fenga and Chi-Yu Lu. |
| Title | Analysis of angiotensin II receptor antagonist and protein markers at microliter level plasma by LC?MS/MS |
| Journal | Journal of Pharmaceutical and Biomedical Analysis.49 123?128. 2009. |
| Authors | Lu CY, Chang YM, Tseng WL, Feng CH, Lu CY. |
ProteoSpin™ Abundant Serum Protein Depletion Kit
This kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples. Such abundant proteins usually obscure less abundant proteins in gel electrophresis making them difficult to visualize and recover. The kit reduces sample complexity to allow for convenient investigation of less abundant proteins. In other applications such as mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins. The kit is 'generic' in that it is not antibody-based and can thus be used to deplete abundant proteins from any starting material - human or various other animals - making it more versatile, flexible and affordable than other kits. Resulting proteins are ready for a range of applications including 2D gel electrophoresis, SDS-PAGE, Mass Spectrometry, Protein microarrays, DIGE (Difference Gel Electrophoresis) and more.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
| Maximum Column Capacity |
500 μg
|
| Minimum Column Capacity |
200 μg
|
| Minimum Elution Volume |
30 μL
|
| Time to Process 10 Samples |
30 minutes
|
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Title | Clinical Proteomics: Liquid Chromatography–Mass Spectrometry (LC–MS) Purification Systems |
| Journal | Protein Chromatography. 2016. |
| Authors | Henry M, Meleady P. |
| Title | Neonatal NET-inhibitory factor and related peptides inhibit neutrophil extracellular trap formation |
| Journal | The Journal of Clinical Investigation. 2016. |
| Authors | Yost CC, Schwertz H, Cody MJ, Wallace JA, Campbell RA, Vieira-de-Abreu A, Araujo CV, Schubert S, Harris ES, Rowley JW, Rondina MT, Fulcher JM, Koening CL, Weyrich AS, Zimmerman GA |
| Title | Doublecortin-Like Kinase 1 Is Elevated Serologically in Pancreatic Ductal Adenocarcinoma and Widely Expressed on Circulating Tumor Cells |
| Journal | PLoS ONE. 2015. |
| Authors | Dongfeng Qu, Jeremy Johnson, Parthasarathy Chandrakesan, Nathaniel Weygant, Randal May, Nicole Aiello, Andrew Rhim, Lichao Zhao, Wei Zheng, Stanley Lightfoot, Shubham Pant, Jeremy Irvan, Russell Postier, James Hocker, Jay S. Hanas, Naushad Ali, Sripathi M. Sureban, Guangyu An, Michael J. Schlosser, Ben Stanger, Courtney W. Houchen |
| Title | Identification of plasma Complement C3 as a potential biomarker for neuroblastoma using a quantitative proteomic approach. |
| Journal | Journal of Proteomics. 2013. |
| Authors | Kim PY, Tan O, Diakiw S, Carter D, Sekerye EO, Wasinger VC, Liu T, Kavallaris M, Norris MD, Haber M, Chesler L, Dolnikov A, Trahair TN, Cheung NK, Marshall GM, Cheung BB. |
| Title | Quantitation of irbesartan and major proteins in human plasma by mass spectrometry with time-of-flight analyzer. |
| Journal | Journal of Pharmaceutical and Biomedical Analysis. 2011. |
| Authors | Lu CY, Feng CH. |
| Title | Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals. |
| Journal | Analytical Biochemistry. 2010. |
| Authors | Kanekoa H, Kamiieb J, Kawakamib H, Anadac T, Hondac Y, Shiraishid N, Kamakurae S, Terasakif T, Shimauchia H, Suzukic O. |
| Title | Modification of major plasma proteins by acrylamide and glycidamide: preliminary screening by nano liquid chromatography with tandem mass spectrometry. |
| Journal | Analytica Chimica Acta. 2010. |
| Authors | Chia-Hsien Fenga and Chi-Yu Lu. |
| Title | Analysis of angiotensin II receptor antagonist and protein markers at microliter level plasma by LC?MS/MS |
| Journal | Journal of Pharmaceutical and Biomedical Analysis.49 123?128. 2009. |
| Authors | Lu CY, Chang YM, Tseng WL, Feng CH, Lu CY. |