ProteoSpin™ Abundant Serum Protein Depletion Kit For the rapid depletion of abundant proteins from serum samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz ProteoSpin™ Abundant Serum Protein Depletion Kit For the rapid depletion of abundant proteins from serum samples Price USD$285.00 SKU 17300 Format Spin Column Size 25 Preps Quantity Overview Details Documentation Citations Overview Rapid and efficient removal of abundant proteins from serum and plasma samples Process 10 samples in 30 minutes Allows for visualization of low abundance proteins Convenient and affordable spin column protocol Generic protocol allows for depletion from human and other animal samples Purification is based on spin column chromatography that uses Norgen’s resin separation matrix This kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples. Such abundant proteins usually obscure less abundant proteins in gel electrophresis making them difficult to visualize and recover. The kit reduces sample complexity to allow for convenient investigation of less abundant proteins. In other applications such as mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins. The kit is 'generic' in that it is not antibody-based and can thus be used to deplete abundant proteins from any starting material - human or various other animals - making it more versatile, flexible and affordable than other kits. Resulting proteins are ready for a range of applications including 2D gel electrophoresis, SDS-PAGE, Mass Spectrometry, Protein microarrays, DIGE (Difference Gel Electrophoresis) and more. Details Supporting Data Previous Figure 1. Depletion of Abundant Proteins. Abundant proteins were depleted from human serum using the ProteoSpinTM Abundant Serum Protein Depletion Kit. Lane 1 represents the input human serum proteins, while Lanes 3 and 4 represent the elution after the abundant proteins were depleted with the kit. Lane 2 represents the binding flowthrough, and contains the proteins that have been depleted from the serum sample. Figure 2. Depletion of Abundant Proteins. A 2D gel was run after the abundant serum proteins were depleted from human serum using the ProteoSpinTM Abundant Serum Protein Depletion Kit. The abundant proteins including albumin, a-antitrypsin, transferin and haptoglobin have been removed from the sample, allowing the visualization and detection of the lower abundance proteins of interest. Next Click for expanded view Kit Specifications Maximum Column Capacity 500 μg Minimum Column Capacity 200 μg Minimum Elution Volume 30 μL Time to Process 10 Samples 30 minutes Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment. Component Cat. 17300 (25 preps) Wash Solution G 125 mL Elution Buffer C 8 mL Protein Neutralizer 4 mL Mini Spin Columns 25 Collection Tubes 25 Elution Tubes (1.7 mL) 25 Product Insert 1 Documentation PROTOCOLS PI17300-12 Serum Depletion Kit Insert.pdf SAFETY DATA SHEETS 17300_17350_SDS.pdf FLYERS Detergent-Free Total Protein Isolation Kit.pdf RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Powered by Bioz See more details on Bioz Related Products ProteoSpin™ Total Protein Concentration and Detergent Clean-Up Mini Kit
ProteoSpin™ Abundant Serum Protein Depletion Kit For the rapid depletion of abundant proteins from serum samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz ProteoSpin™ Abundant Serum Protein Depletion Kit For the rapid depletion of abundant proteins from serum samples Price USD$285.00 SKU 17300 Format Spin Column Size 25 Preps Quantity