• Detection kits for the Aspergillus niger
  • Available in 2 formats for analysis:
 

ItemsCat. #Size
Aspergillus niger TaqMan PCR Kit TM32900 24 rxns
Aspergillus niger TaqMan Probe/Primer and Control Set TM32910 100 rxns
Aspergillus niger End-Point PCR Kit EP32900 24 rxns
Aspergillus niger Primer and Control Set EP32910 100 rxns

Aspergillus niger Detection Kits

Aspergillus niger is a fungus and one of the most common species of the genus Aspergillus. It causes a disease called black mold on certain fruits and vegetables such as grapes, onions, and peanuts, and is a common contaminant of food. It causes stem rot of Dracaena, root stalk rot of Sansevieria and boll rot of cotton. It has also been implicated in the spoilage of cashew kernels, dates, figs, vanilla pods and dried prunes. It is ubiquitous in soil and is commonly reported from indoor environments, where it produces characteristic black colonies. Some strains of A. niger have also been reported to produce potent mycotoxins called ochratoxins. Methods for the rapid and sensitive detection of the pest would be valuable to ensure food quality and protection of individuals from the possibility of ingesting potentially hazardous myco- or ochratoxins.

There are 2 kits available for the detection of Aspergillus niger:

Aspergillus niger TaqMan PCR Kit
  • PCR control to monitor for PCR inhibition and validate the quality
  • Master Mix for the target and PCR control detection
  • Primer and Probe mix
  • Positive control and a negative control to confirm the integrity of the kit reagents
Aspergillus niger End-Point PCR Kit
  • Master Mix and primers for the specific amplification of a 363 nucleotide region of the Aspergillus niger genome
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


Kit Components
Component TaqMan Probe
Cat. TM32900
(24 rxns)
End-Point
Cat. EP32900
(24 rxns)
MDx 2X PCR Master Mix - 350 µL
MDx TaqMan 2X PCR Master Mix 350 µL -
Aspergillus niger Primer Mix 70 µL 70 µL
Aspergillus niger Positive Control 50 µL 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Loading Dye - 100 µL
DNA Ladder - 100 µL
Product Insert 1 1
Component TaqMan
Probe/Primer
and Control Set
Cat. TM32910
(100 rxns)
Primer
and Control Set
Cat. EP32910
(100 rxns)
Aspergillus niger Primer Mix 3 x 70 µL 3 x 70 µL
Aspergillus niger Positive Control 3 x 50 µL 3 x 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1