Botrytis cinerea TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Botrytis cinerea TaqMan PCR Detection Kits Price USD$699.00 SKU TM29450 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Botrytis cinerea Available in TaqMan format for analysis Botrytis cinerea is a ubiquitous fungal pathogen, often present as a latent infection, which can in some cases later develops into damaging symptomatic infections in a wide range of plant species including economically important crops such as vegetables, ornamentals, bulbs and fruits. Consequently, an apparently healthy plant can deteriorate suddenly due to the development of latent or quiescent infection into visible disease. Sensitive and reliable methods to monitor the fungus in plant tissue are essential to enable the study of the factors affecting the progression from latent to aggressive disease, and to allow for early detection and diagnosis of the disease. TaqMan PCR Kit Click to expand options Botrytis cinerea TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Botrytis cinerea TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM29450 (100 preps) Cat. TM29410 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - B. cinerea Primer & Probe Mix 280 μL 280 μL B. cinerea Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM29450) Botrytis cinerea TaqMan PCR Kit - Protocol (100 rxns) SAFETY DATA SHEETS (TM29450) Botrytis cinerea TaqMan PCR Kit - Safety Data Sheet Related Products Fungi/Yeast Genomic DNA Isolation Kit
Botrytis cinerea TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Botrytis cinerea TaqMan PCR Detection Kits Price USD$699.00 SKU TM29450 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity
