Citrus Exocortis Viroid (CEVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Citrus Exocortis Viroid (CEVd) TaqMan RT-PCR Detection Kits SKU TM47650 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Contact Distributor Overview Details Documentation Citations Overview Detection kits for the CEVd Available in TaqMan format for analysis Citrus Exocortis Viroid (CEVd) is a member of the genus Pospiviroid within the family Pospiviroidae. CEVd is the causal agent of the exocortis disease characterized by a bark shelling or scaling disorder of trifoliate orange used as rootstock. The citrange hybrids and Rangpur lime, both used as rootstocks, are also sensitive and develop bark scaling symptoms and stunting. Viroids are small plant pathogens consisting of a naked single-stranded circular RNA molecule of 246–475 nt, that do not encode any proteins but are endowed with autonomous replication in their host plants. Norgen’s Citrus Exocortis Viroid RT-PCR Detection Kit is a rapid and sensitive CEVd detection tool which can be applicable for the early diagnosis of CEVd. TaqMan PCR Kit Click to expand options CEVd TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents CEVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM47650 (100 preps) Cat. TM47610 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - CEVd Primer & Probe Mix 280 μL 280 μL CEVd Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM47650) CEVd TaqMan RT-PCR Kit - Protocol (100 rxns) Related Products Citrus Exocortis Viroid (CEVd) TaqMan Probe/Primer and Control Set Plant RNA/DNA Purification Kit Plant/Fungi Total RNA Purification Kit
Citrus Exocortis Viroid (CEVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Citrus Exocortis Viroid (CEVd) TaqMan RT-PCR Detection Kits SKU TM47650 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Contact Distributor
