|Detergent Free Total Protein Isolation Kit||30300||25 preps|
Detergent-Free Total Protein Isolation Kit
This kit provides a fast and simple procedure for the isolation of total proteins from tissue, bacteria, yeast or mammalian cells without the use of SDS, Triton® X-100 and other detergents. Detergents are extensively used to prepare protein samples; however, these detergents have undesirable effects on downstream analysis. These effects include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization.
The Detergent-Free Total Protein Isolation Kit maintains high protein recovery and yields proteins that are 100% detergent-free. Purification is based on using Norgen’s proprietary resin together with Lysis Solution, followed by protein filtration using a filter column (provided). The purified proteins can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, NMR spectroscopy and more.
Each Lysis Tube is able to process up to 50 mg of tissue, 1010 bacterial cells, 109 yeast cells or 107 mammalian cells. Preparation time for 12 samples is less than 10 minutes.
|Maximum Amount Of Starting Material:
1 x 107 cells
1 x 1010 cells
1 x 109 cells
|Time to Process 12 Samples||Less than 10 minutes|
Storage Conditions and Product Stability
The Lysis Solution should be kept tightly sealed and stored at room temperature.
|Title||Molecular and Proteomic Analysis of Levofloxacin and Metronidazole Resistant Helicobacter pylori|
|Journal||Frontiers in Microbology. 2016.|
|Authors||Hanafi A, Lee WC, Loke MF, Teh X, Shaari A, Dinarvand M, Lehours P, Mégraud F, Leow AH, Vadivelu J, Goh KL.|
|Title||Proteomics Analysis Revealed that Crosstalk between Helicobacter pylori and Streptococcus mitis May Enhance Bacterial Survival and Reduces Carcinogenesis|
|Journal||Frontiers in Microbiology. 2016.|
|Authors||Khosravi Y, Loke MF, Goh KL, Vadivelu J.|
|Title||Multiphasic strain differentiation of atypical mycobacteria from elephant trunk wash|
|Authors||Kok-Gan Chan, Mun Fai Loke, Bee Lee Ong, Yan Ling Wong, Kar Wai Hong, Kian Hin Tan, Sargit Kaur, Hien Fuh Ng, MFA Abdul Razak, Yun Fong Ngeow|
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