Detergent-Free Total Protein Isolation Kit Fast and simple detergent-free isolation of total proteins For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Detergent-Free Total Protein Isolation Kit Fast and simple detergent-free isolation of total proteins SMART Save USD$169.00 USD$135.20 SKU 30300 Size 25 Preps Quantity Overview Details Documentation Citations Overview Simple protocol that is free of detergents Fast processing time Process a variety of inputs Compatible with mass spectrometry and NMR spectroscopy Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit provides a fast and simple procedure for the isolation of total proteins from tissue, bacteria, yeast or mammalian cells without the use of SDS, Triton® X-100 and other detergents. Detergents are extensively used to prepare protein samples; however, these detergents have undesirable effects on downstream analysis. These effects include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization. The Detergent-Free Total Protein Isolation Kit maintains high protein recovery and yields proteins that are 100% detergent-free. Purification is based on using Norgen’s proprietary resin together with Lysis Solution, followed by protein filtration using a filter column (provided). The purified proteins can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, NMR spectroscopy and more.Each Lysis Tube is able to process up to 50 mg of tissue, 1010 bacterial cells, 109 yeast cells or 107 mammalian cells. Preparation time for 12 samples is less than 10 minutes. Details Supporting Data Previous Figure 1. Detergent-free Proteins Isolated from E. coli, Yeast, HeLa Cells and Kidney Tissue. Total proteins were isolated from 2 x 109 E. coli cells, 2 x 107 yeast cells, 1 million HeLa cells and 15 mg of Chinese hamster kidney tissue in duplicate using Norgen’s Detergent-Free Total Protein Isolation Kit. The total proteins were analyzed by running aliquots on 12% SDS-PAGE gels. Panel A corresponds to 10 µL of the 40 µL recovered proteins from E. coli., Panel B corresponds to 10 µL of the 40 µL recovered proteins from yeast, Panel C corresponds to 12 µL of the 60 µL recovered proteins from the HeLa cells, while Panel D corresponds to 2 µL of the 60 µL of recovered proteins from the kidney tissue. The proteins are intact and of the highest quality, and can be used in a number of different downstream applications. Next Click for expanded view Kit Specifications Maximum Amount Of Starting Material: Tissues Animal Cells Bacteria Yeast 50 mg 1 x 107 cells 1 x 1010 cells 1 x 109 cells Time to Process 12 Samples Less than 10 minutes Storage Conditions and Product Stability The Lysis Solution should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment. Component Cat. 30300 (50 preps) Lysis Solution 4 mL Lysis Tubes 25 Filter Columns 25 Elution Tubes (1.7 mL) 25 Product Insert 1 Documentation PROTOCOLS (30300) Detergent-Free Total Protein Isolation Kit - Protocol (25 prep) SAFETY DATA SHEETS (30300) Detergent-Free Total Protein Isolation Kit - Safety Data Sheet FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Title Sperm morphology and performance in relation to postmating prezygotic isolation in two recently diverged passerine species Journal Scientific Reports. 2022. Authors Poignet, M., Baránková, L., Reif, J. et al. Title Comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds Journal Scientific Reports. 2021. Authors Voukali, E., Veetil, N.K., Němec, P. et al. Title Molecular and Proteomic Analysis of Levofloxacin and Metronidazole Resistant Helicobacter pylori Journal Frontiers in Microbology. 2016. Authors Hanafi A, Lee WC, Loke MF, Teh X, Shaari A, Dinarvand M, Lehours P, Mégraud F, Leow AH, Vadivelu J, Goh KL. Title Proteomics Analysis Revealed that Crosstalk between Helicobacter pylori and Streptococcus mitis May Enhance Bacterial Survival and Reduces Carcinogenesis Journal Frontiers in Microbiology. 2016. Authors Khosravi Y, Loke MF, Goh KL, Vadivelu J. Title Multiphasic strain differentiation of atypical mycobacteria from elephant trunk wash Journal PeerJ. 2015. Authors Kok-Gan Chan, Mun Fai Loke, Bee Lee Ong, Yan Ling Wong, Kar Wai Hong, Kian Hin Tan, Sargit Kaur, Hien Fuh Ng, MFA Abdul Razak, Yun Fong Ngeow
Detergent-Free Total Protein Isolation Kit Fast and simple detergent-free isolation of total proteins For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Detergent-Free Total Protein Isolation Kit Fast and simple detergent-free isolation of total proteins SMART Save USD$169.00 USD$135.20 SKU 30300 Size 25 Preps Quantity
