Fast and simple detergent-free isolation of total proteins

  • Simple protocol that is free of detergents
  • Fast processing time
  • Process a variety of inputs
  • Compatible with mass spectrometry and NMR spectroscopy

ItemsCat. #Size
Detergent Free Total Protein Isolation Kit 30300 25 preps

Detergent-Free Total Protein Isolation Kit

This kit provides a fast and simple procedure for the isolation of total proteins from tissue, bacteria, yeast or mammalian cells without the use of SDS, Triton® X-100 and other detergents. Detergents are extensively used to prepare protein samples; however, these detergents have undesirable effects on downstream analysis. These effects include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization.

The Detergent-Free Total Protein Isolation Kit maintains high protein recovery and yields proteins that are 100% detergent-free. Purification is based on using Norgen’s proprietary resin together with Lysis Solution, followed by protein filtration using a filter column (provided). The purified proteins can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, NMR spectroscopy and more.

Each Lysis Tube is able to process up to 50 mg of tissue, 1010 bacterial cells, 109 yeast cells or 107 mammalian cells. Preparation time for 12 samples is less than 10 minutes.

Kit Specifications
Maximum Amount Of Starting Material:
Tissues
Animal Cells
Bacteria
Yeast
50 mg
1 x 107 cells
1 x 1010 cells
1 x 109 cells
Time to Process 12 Samples Less than 10 minutes

Storage Conditions and Product Stability
The Lysis Solution should be kept tightly sealed and stored at room temperature.

Title Molecular and Proteomic Analysis of Levofloxacin and Metronidazole Resistant Helicobacter pylori
Journal Frontiers in Microbology. 2016.
Authors Hanafi A, Lee WC, Loke MF, Teh X, Shaari A, Dinarvand M, Lehours P, Mégraud F, Leow AH, Vadivelu J, Goh KL.
Title Multiphasic strain differentiation of atypical mycobacteria from elephant trunk wash
Journal PeerJ. 2015.
Authors Kok-Gan Chan, Mun Fai Loke, Bee Lee Ong, Yan Ling Wong, Kar Wai Hong, Kian Hin Tan, Sargit Kaur, Hien Fuh Ng, MFA Abdul Razak, Yun Fong Ngeow​