Direct DNA Extraction Kit (Bacteria)

Cat. 61500
  • Professional Tech Support with Senior R&D Scientist
  • Fast and Flexible Ordering Options (online, email, or phone)
  • Volume Discounts Available on Large Quantity Orders*

*Inquire by phone or email for more information.


Direct DNA Extraction Kit (Bacteria)

Cat. 61500

For the extraction of DNA from enriched Gram negative and positive bacteria from food and culture samples

  • Rapid procedure
  • Easy and convenient protocol
  • Reproducible
  • Applicable for high throughput detection platforms

For research use only and NOT intended for in vitro diagnostics.

Want a discount?

Create your account to receive 15% off on your first purchase!

Direct DNA Extraction Kit (Bacteria)
(Cat. 61500)
50 preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 61500

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Title Biodegradation of low density polyethylene (LDPE) modified with dye sensitized titania and starch blend using Stenotrophomonas pavanii
Journal International Biodeterioration & Biodegradation. 2016.
Authors Ch. Tahir Mehmood, Ishtiaq A. Qazi, Imran Hashmi, Samarth Bhargava, Sriramulu Deepa
Title Microbial population dynamics and profiling of quorum sensing agents in membrane bioreactor
Journal International Biodeterioration & Biodegradation. 2015.
Authors Hira Waheed, Imran Hashmi, Sher Jamal Khan, Sang Ryoung Kim, Muhammad Arshad, Habib Nasir

Direct DNA Extraction Kit (Bacteria)

Norgen's Direct DNA Extraction Kit (Bacteria) contains a Direct Pathogen Extraction Buffer (Buffer DL) and pre-filled bead tubes for the extraction of DNA from enriched Gram negative and positive bacteria from food and culture samples. The kit first allows for the extraction of DNA directly from bacteria using mechanical homogenization with Buffer DL. The cell debris is then removed by a short centrifugation and the clean supernatant is ready for use in PCR reactions. Buffer DL is designed to stabilize extracted DNA and inactivate PCR inhibitors to provide a rapid and economic solution for high throughput pathogen detection, particularly for Gram-positive bacteria such as Listeria spp. Norgen's Direct DNA Extraction Kit (Bacteria) is compatible with sensitive detection applications including end-point PCR and real-time PCR.

Direct DNA Extraction Kit (Bacteria)
Procedure

Direct DNA Extraction Step 1
1. Take 1 mL from enriched culture
Direct DNA Extraction Step 2
2. Pellet the cells and resuspend with 400 µL of Buffer DL
Direct DNA Extraction Step 3
3. Homogenize in a bead beater
Direct DNA Extraction Step 4
4. Remove the cell debris by centrifugation
Direct DNA Extraction Step 5
5. The clean supernatant can be used directly in PCR

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 61500

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Title Biodegradation of low density polyethylene (LDPE) modified with dye sensitized titania and starch blend using Stenotrophomonas pavanii
Journal International Biodeterioration & Biodegradation. 2016.
Authors Ch. Tahir Mehmood, Ishtiaq A. Qazi, Imran Hashmi, Samarth Bhargava, Sriramulu Deepa
Title Microbial population dynamics and profiling of quorum sensing agents in membrane bioreactor
Journal International Biodeterioration & Biodegradation. 2015.
Authors Hira Waheed, Imran Hashmi, Sher Jamal Khan, Sang Ryoung Kim, Muhammad Arshad, Habib Nasir

Related Products