Direct DNA Extraction Kit (Bacteria) For the extraction of DNA from enriched Gram negative and positive bacteria from food and culture samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Direct DNA Extraction Kit (Bacteria) For the extraction of DNA from enriched Gram negative and positive bacteria from food and culture samples Price USD$89.00 SKU 61500 Format Bead Tube Size 50 Preps Quantity Overview Details Documentation Citations Overview Rapid procedureEasy and convenient protocolReproducibleApplicable for high throughput detection platforms Norgen's Direct DNA Extraction Kit (Bacteria) contains a Direct Pathogen Extraction Buffer (Buffer DL) and pre-filled bead tubes for the extraction of DNA from enriched Gram negative and positive bacteria from food and culture samples. The kit first allows for the extraction of DNA directly from bacteria using mechanical homogenization with Buffer DL. The cell debris is then removed by a short centrifugation and the clean supernatant is ready for use in PCR reactions. Buffer DL is designed to stabilize extracted DNA and inactivate PCR inhibitors to provide a rapid and economic solution for high throughput pathogen detection, particularly for Gram-positive bacteria such as Listeria spp. Norgen's Direct DNA Extraction Kit (Bacteria) is compatible with sensitive detection applications including end-point PCR and real-time PCR.Direct DNA Extraction Kit (Bacteria)Procedure1. Take 1 mL from enriched culture 2. Pellet the cells and resuspend with 400 µL of Buffer DL 3. Homogenize in a bead beater 4. Remove the cell debris by centrifugation 5. The clean supernatant can be used directly in PCR Details Supporting Data Previous Figure 1. Detection of Gram negative (E.coli) and Gram positive (Listeria spp.) bacteria using Norgen's Direct DNA Extraction Kit (Bacteria) in real-time PCR system. Two microlitres of the clean supernatant was directly added to a PCR reaction (total 20 µL) to detect 16s rRNA target or Listeria spp. specific gene for E.coli and Listeria spp. respectively. Targets were successfully amplified, indicating the high quality of the inhibitor-free DNA that was extracted using Norgen's Direct DNA Extraction Kit (Bacteria). This kit is applicable for rapid and sensitive microorganism detection for food quality monitoring and other high throughput analysis applications. Next Click for expanded view Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. ComponentCat. 61500 (50 preps)Buffer DL2 x 25 mLBead Tubes50Product Insert1 Documentation PROTOCOLS (61500) Direct DNA Extraction Kit (Bacteria) - Protocol (50 prep) SAFETY DATA SHEETS (61500) Direct DNA Extraction Kit (Bacteria - Safety Data Sheet Citations Title Biodegradation of low density polyethylene (LDPE) modified with dye sensitized titania and starch blend using Stenotrophomonas pavanii Journal International Biodeterioration & Biodegradation. 2016. Authors Ch. Tahir Mehmood, Ishtiaq A. Qazi, Imran Hashmi, Samarth Bhargava, Sriramulu Deepa Title Microbial population dynamics and profiling of quorum sensing agents in membrane bioreactor Journal International Biodeterioration & Biodegradation. 2015. Authors Hira Waheed, Imran Hashmi, Sher Jamal Khan, Sang Ryoung Kim, Muhammad Arshad, Habib Nasir
Direct DNA Extraction Kit (Bacteria) For the extraction of DNA from enriched Gram negative and positive bacteria from food and culture samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Direct DNA Extraction Kit (Bacteria) For the extraction of DNA from enriched Gram negative and positive bacteria from food and culture samples Price USD$89.00 SKU 61500 Format Bead Tube Size 50 Preps Quantity