DNA Clean-Up and Concentration Micro-Elute Kit For the purification, cleanup and concentration of DNA For research use only and NOT intended for in vitro diagnostics. Powered by Bioz DNA Clean-Up and Concentration Micro-Elute Kit For the purification, cleanup and concentration of DNA Price USD$129.00 SKU 67200 Format Micro-Elute Size 50 Preps Quantity Overview Details Documentation Citations Overview Rapid concentration of small amounts of DNA into flexible final elution volumes of 8 to 15 μLIdeal for concentrating DNA from PCR and other enzymatic or labelling reactions and cleanup of plasmids or DNA previously isolated by other methodsIsolated DNA is suitable for any downstream application including PCR, sequencing, ligation, RNA transcription, radiolabeling, arrays and more Norgen’s DNA Clean-Up and Concentration Micro-Elute Kit provides a rapid method for the purification, cleanup and concentration of up to 40 μg of DNA from PCR reactions, endonuclease digestions as well as from DNA modification reactions, labeling reactions and more. Plasmids <10,000 bp in size and genomic DNA may also be concentrated. The minimum recommended elution volume is 8 μL, which enables the concentration of small amounts of all sizes of DNA. The DNA is preferentially purified from other reaction components such as proteins, nucleases and free nucleotides or other reaction components. The purified DNA is free of salts and contaminants and is of the highest integrity ready for use in PCR, DNA sequencing, ligation reactions, endonuclease digestion, radiolabeling, arrays and more. Preparation time for a single sample is approximately 20 minutes, and each kit contains sufficient materials for 50 preparations. Details Supporting Data Previous Figure 1: Efficient Concentration of DNA from Various Sources. Approximately 25 µg of plasmid DNA (panel A), HEK 293 cell line DNA (panel B), or PCR product (panel C) was concentrated using the DNA Clean-Up and Concentration Micro-Elute Kit and eluted into a final volume of 15 µL. For each sample, 1 µL of unconcentrated (U) and concentrated DNA (C) was then run on an 1.3% agarose gel at 170 V for 25 min. In each case the DNA Clean-Up & Concentration Micro-Elute Kit demonstrates a significantly higher final concentration of DNA. M = Norgen HighRanger DNA Ladder (Cat#11900). Next Click for expanded view Norgen’s Purification Technology Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The DNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first mixing the DNA samples or enzymatic reactions containing DNA with Buffer RL. Ethanol is then added and the mixture is loaded onto an activated spin-column specifically designed for small elution volumes. Norgen’s resin binds DNA in a manner that depends on ionic concentrations. Thus only the DNA will bind to the column, while the contaminating proteins or free nucleotides will be removed in the flowthrough. The bound DNA is then washed three times with the provided Wash Solution A in order to remove any remaining impurities. The purified DNA is then eluted into a small volume (8 –15 μL) using Elution Buffer F. Kit Specifications Maximum Binding Capacity 40 μg of DNA Size of DNA Purified 50 bp to 10,000 bp Maximum Volume of Starting Material 200 μL Minimum Elution Volume 8 μL Time to Complete 10 Purifications 20 minutes Average Recovery ≥ 90% Advantages Concentration of small amounts of DNA into 8 to 15 μL Ideal for concentrating DNA from PCRs and other enzymatic labeling reactions No need for organic denaturants or chloroform Cleanup of plasmids or DNA isolated using other DNA isolation methods Fast and easy processing using rapid spin-column format Suitable for DNA from 50 bp to 10,000 bp Component Cat. 67200 (50 preps) Buffer RL 40 mL Wash Solution A 38 mL Elution Buffer F 6 mL Column Activation Solution 30 mL Micro-Elute DNA Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened container. Documentation PROTOCOLS (67200) DNA Clean-Up and Concentration Micro-Elute Kit - Protocol (50 preps) Citations Title Functional validation of UGT1A6 in anthracycline-induced cardiotoxicity Journal UBC Theses and Dissertations. 2022. Authors Morrison, Alexander Title Characterization of the urogenital microbiome in Miniature Schnauzers with and without calcium oxalate urolithiasis Journal Journal of Veterinary Internal Medicine. 2022. Authors Emily L. Coffey,Andres M. Gomez,Erin N. Burton,Jennifer L. Granick,Jody P. Lulich,Eva Furrow Related Products RNA Clean-Up and Concentration Micro-Elute Kit PCR Purification Kit
DNA Clean-Up and Concentration Micro-Elute Kit For the purification, cleanup and concentration of DNA For research use only and NOT intended for in vitro diagnostics. Powered by Bioz DNA Clean-Up and Concentration Micro-Elute Kit For the purification, cleanup and concentration of DNA Price USD$129.00 SKU 67200 Format Micro-Elute Size 50 Preps Quantity
