Name: DNA Clean-Up and Concentration Micro-Elute Kit
Brand: Norgen Biotek Corp.
SKU: 67200
Price: 129.000000 USD
Availability: InStock

Features and Benefits

Rapid concentration of small amounts of DNA into flexible final elution volumes of 8 to 15 μL
Ideal for concentrating DNA from PCR and other enzymatic or labelling reactions and cleanup of plasmids or DNA previously isolated by other methods
Isolated DNA is suitable for any downstream application including PCR, sequencing, ligation, RNA transcription, radiolabeling, arrays and more

Norgen’s DNA Clean-Up and Concentration Micro-Elute Kit provides a rapid method for the purification, cleanup and concentration of up to 40 μg of DNA from PCR reactions, endonuclease digestions as well as from DNA modification reactions, labeling reactions and more. Plasmids <10,000 bp in size and genomic DNA may also be concentrated. The minimum recommended elution volume is 8 μL, which enables the concentration of small amounts of all sizes of DNA. The DNA is preferentially purified from other reaction components such as proteins, nucleases and free nucleotides or other reaction components. The purified DNA is free of salts and contaminants and is of the highest integrity ready for use in PCR, DNA sequencing, ligation reactions, endonuclease digestion, radiolabeling, arrays and more. Preparation time for a single sample is approximately 20 minutes, and each kit contains sufficient materials for 50 preparations.

Details

Supporting Data

Figure 1: Efficient Concentration of DNA from Various Sources. Approximately 25 µg of plasmid DNA (panel A), HEK 293 cell line DNA (panel B), or PCR product (panel C) was concentrated using the DNA Clean-Up and Concentration Micro-Elute Kit and eluted into a final volume of 15 µL. For each sample, 1 µL of unconcentrated (U) and concentrated DNA (C) was then run on an 1.3% agarose gel at 170 V for 25 min. In each case the DNA Clean-Up & Concentration Micro-Elute Kit demonstrates a significantly higher final concentration of DNA. M = Norgen HighRanger DNA Ladder (Cat#11900).

Norgen’s Purification Technology

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The DNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first mixing the DNA samples or enzymatic reactions containing DNA with Buffer RL. Ethanol is then added and the mixture is loaded onto an activated spin-column specifically designed for small elution volumes. Norgen’s resin binds DNA in a manner that depends on ionic concentrations. Thus only the DNA will bind to the column, while the contaminating proteins or free nucleotides will be removed in the flowthrough. The bound DNA is then washed three times with the provided Wash Solution A in order to remove any remaining impurities. The purified DNA is then eluted into a small volume (8 –15 μL) using Elution Buffer F.

Kit Specifications
Maximum Binding Capacity	40 μg of DNA
Size of DNA Purified	50 bp to 10,000 bp
Maximum Volume of Starting Material	200 μL
Minimum Elution Volume	8 μL
Time to Complete 10 Purifications	20 minutes
Average Recovery	≥ 90%

Advantages

Concentration of small amounts of DNA into 8 to 15 μL
Ideal for concentrating DNA from PCRs and other enzymatic labeling reactions
No need for organic denaturants or chloroform
Cleanup of plasmids or DNA isolated using other DNA isolation methods
Fast and easy processing using rapid spin-column format
Suitable for DNA from 50 bp to 10,000 bp

Component	Cat. 67200 (50 preps)
Buffer RL	40 mL
Wash Solution A	38 mL
Elution Buffer F	6 mL
Column Activation Solution	30 mL
Micro-Elute DNA Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened container.

Documentation

PROTOCOLS

(67200) DNA Clean-Up and Concentration Micro-Elute Kit - Protocol (50 preps)

SAFETY DATA SHEETS

67200 - DNA Clean-Up and Concentration Micro-Elute Kit

FAQs

Micro-Elute

Why do I have poor DNA recovery?

Poor DNA recovery could be due to one or a combination of the following factors:

Column has become clogged.
Do not exceed the recommended amounts of starting materials. The amount of starting material may need to be decreased if the column shows clogging below the recommended levels.
An alternative elution solution was used.
It is recommended that the Elution Buffer F supplied with this kit be used for maximum DNA recovery.
Ethanol was not added to the lysate.
Ensure that the appropriate amount of ethanol is added to the lysate before binding to the column.
Ethanol was not added to the Wash Solution A.
Ensure that 90 mL of 96 - 100% ethanol is added to the supplied Wash Solution A prior to use.

I noticed the column was clogged. What causes this?

Column clogging can result from one or a combination of the following factors:

High amounts of DNA in the input.
Ensure that no more than 40 μg of DNA are used as the input for each column.
Centrifuge temperature too low.
Ensure that the centrifuge remains at room temperature throughout the procedure. Temperatures below 15°C may cause precipitates to form that can cause the columns to clog.

Why is the eluted DNA degraded?

Eluted DNA can be degraded due to the following factors:

DNase contamination.
DNases may be introduced during the use of the kit. Ensure proper procedures are followed when working with DNA.
Starting size of DNA > 10,000 bp.
When purifying large plasmids > 10,000 bp in size, DNA may be sheared into smaller fragments.
Improper storage of the purified DNA.
DNA samples may be stored at 4°C for a few days. It is recommended that samples be stored at –20°C for longer term storage.

Why is the purified DNA not performing well in downstream applications?

Presence of impurities from the sample or the protocol can affect the performance of DNA in downstream applications. This can happen when:

DNA was not washed three times with the provided Wash Solution A.
Traces of salt from the binding step may remain in the sample if the column is not washed three times with the Wash Solution A. Salt may interfere with downstream applications, and thus must be washed from the column.
There is ethanol carryover.
Ensure that the dry spin under the Column Wash procedure is performed, in order to remove traces of ethanol prior to elution. Ethanol is known to interfere with many downstream applications.
Improper pH of elution buffer.
The pH of the elution buffer can affect DNA recovery and downstream performance. Ensure the elution buffer has a pH between 7 and 8.
Inadequate mixing of sample and binding buffer.
Ensure thorough mixing of the sample and binding buffer to facilitate proper binding of DNA to the column.

Citations

Title	Assessing the breeding phenology of a threatened frog species using eDNA and automatic acoustic monitoring
Citation	PeerJ 2023.
Authors	Ying Chen?, Orianne Tournayre?, Haolun Tian, Stephen C. Lougheed?

Title	Biomolecular Features of COVID-19 in Hodeidah, Yemen
Citation	Asian Journal of Biochemistry, Genetics and Molecular Biology 2023.
Authors	Kamarany, Mohammed Amood AL and Abdulkarim, Tarik and Nasser, Mahfouz

DNA Clean-Up and Concentration Micro-Elute Kit