|DNA Clean-Up and Concentration Micro-Elute Kit||67200||50 preps|
DNA Clean-Up and Concentration Micro-Elute Kit
Norgen’s DNA Clean-Up and Concentration Micro-Elute Kit provides a rapid method for the purification, cleanup and concentration of up to 40 μg of DNA from PCR reactions, endonuclease digestions as well as from DNA modification reactions, labeling reactions and more. Plasmids <10,000 bp in size and genomic DNA may also be concentrated. The minimum recommended elution volume is 8 μL, which enables the concentration of small amounts of all sizes of DNA. The DNA is preferentially purified from other reaction components such as proteins, nucleases and free nucleotides or other reaction components. The purified DNA is free of salts and contaminants and is of the highest integrity ready for use in PCR, DNA sequencing, ligation reactions, endonuclease digestion, radiolabeling, arrays and more. Preparation time for a single sample is approximately 20 minutes, and each kit contains sufficient materials for 50 preparations.
Norgen’s Purification Technology
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The DNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first mixing the DNA samples or enzymatic reactions containing DNA with Buffer RL. Ethanol is then added and the mixture is loaded onto an activated spin-column specifically designed for small elution volumes. Norgen’s resin binds DNA in a manner that depends on ionic concentrations. Thus only the DNA will bind to the column, while the contaminating proteins or free nucleotides will be removed in the flowthrough. The bound DNA is then washed three times with the provided Wash Solution A in order to remove any remaining impurities. The purified DNA is then eluted into a small volume (8 –15 μL) using Elution Buffer F.
Maximum Binding Capacity
40 μg of DNA
|Size of DNA Purified||
50 bp to 10,000 bp
|Maximum Volume of Starting Material||
|Minimum Elution Volume||
|Time to Complete 10 Purifications||
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened container.
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