DNA Gel Extraction Kit For rapid extraction of DNA from agarose gel fragments For research use only and NOT intended for in vitro diagnostics. Powered by Bioz DNA Gel Extraction Kit For rapid extraction of DNA from agarose gel fragments Price USD$105.00 SKU 13100 Format Spin Column Size 50 Preps Quantity Overview Details Documentation Citations Overview Fast and easy recovery of DNA from agarose gel fragmentsHigh recovery of desired DNAConvenient spin column formatDNA is ready for ligation, restriction digestion, sequencing and more This kit is designed for the rapid preparation and purification of DNA fragments that have been fractionated on agarose gels. The recovered DNA is free from agarose and other impurities, and is compatible with restriction enzyme digestion, ligation into vectors and sequencing. The protocol can be completed in 20 minutes. Details Supporting Data Previous Figure 1. Efficient Recovery from Agarose Gels. The high yield of Norgen's DNA Gel Extraction Kit is illustrated by purification of 1.5 µg of a 500 bp fragment from a 1% agarose gel using Norgen's DNA Gel Extraction Kit and two other commercial kits. DNA recovery was quantified by running 300 ng (1/5th) of the input and 10 µL (1/5th) of the 50 µL DNA elution on a 1X TAE, 1% agarose gel followed by densitometry. The Norgen kit shows a significantly higher efficiency compared to the competitor kits based on the average of three purifications. Figure 2. Efficient Recovery of Large DNA Fragments. The efficient recovery of Norgen's DNA Gel Extraction Kit is illustrated by purification of 1.5 µg of a 3,700 bp fragment from a 0.9% agarose gel using Norgen's DNA Gel Extraction Kit (Lanes 5-7) and a competitor's kit (Lanes 2-4). Eluted DNA was resolved on a 1X TAE, 0.9% agarose gel. Lane 1 indicates 300 ng (20%) of the input amount, while lanes 2-7 contain 10 µL (20%) of the 50 µL eluted amount. Norgen's kit shows a higher recovery than the competitors kit. Next Click for expanded view Kit Specifications Column Binding Capacity 10 μg Maximum Weight of Gel Slice 400 mg Average Recovery 70-90% Size of DNA Purified 100 - 15,000 bp Minimum Elution Volume 30 μL Time to Complete 10 Purifications 30 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 13100 (50 preps) Binding Buffer G 80 mL Wash Solution A 12 mL Elution Buffer B 8 mL Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (13100) DNA Gel Extraction Kit - Protocol (50 prep) SAFETY DATA SHEETS (13100) DNA Gel Extraction Kit - Safety Data Sheet Citations Title Differences in Gene Expression of Pear Selections Showing Leaf Curling or Leaf Reddening Symptoms Due to Pear Decline Phytoplasma Journal Plants (Basel). 2022. Authors Mina Kaviani, Paul H Goodwin, David M Hunter Title High genetic diversity of human rhinovirus among pilgrims with acute respiratory tract infections during the 2019 Hajj pilgrimage season Journal International Journal of Infectious Diseases. 2022 Authors Sherif A.El-Kafrawy, Salma M.Alsayed, Thamir A.Alandijany, Leena H.Bajrai, Arwa A.Faizo, Hessa A.Al-Sharif, Ahmed M.Hassan, Khalid M.Alquthami, Jaffar A.Al-Tawfiq, AlimuddinZumla, Esam I.Azhar Title Thrombopoietin Secretion by Human Ovarian Cancer Cells Journal International Journal of Cell Biology. 2017 Authors Samaher Besbes, Shahid Shah, Iman Al-dybiat, Shahsoltan Mirshahi, Helene Helfer, Haythem Najah, Caroline Fourgeaud, Marc Pocard, Ibtissem Ghedira, Jeannette Soria, and Massoud Mirshahi Title Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects Journal Journal of Crop Protection. 2015. Authors Masoumeh Kordi, Naser Farrokhi, Abolfazl Masoudi, Ali Derakhshan Shadmehri and Shahrokh Gharanjik Title Host Exopolysaccharide Quantity and Composition Impact Erwinia amylovora Bacteriophage Pathogenesis. Journal Applied and Environmental Microbiology. 2013. Authors Roach DR, Sjaarda DR, Castle AJ, Svircev AM. Title Natural occurrence of entomopathogenic nematode species (Rhabditida: Steinernematidae and Heterorhabditidae) in cotton fields of Tamil Nadu, India. Journal Journal of Natural History. 2012. Authors Seenivasana N, Prabhub S, Makeshc S, Sivakumara M. Title Isolation and characterization of superior rumen bacteria of cattle (Bos taurus) and potential application in animal feedstuff. Journal Open Journal of Animal Sciences. 2012. Authors Krushna Chandra Das, Wensheng Qin. Related Products PCR Purification Kit
DNA Gel Extraction Kit For rapid extraction of DNA from agarose gel fragments For research use only and NOT intended for in vitro diagnostics. Powered by Bioz DNA Gel Extraction Kit For rapid extraction of DNA from agarose gel fragments Price USD$105.00 SKU 13100 Format Spin Column Size 50 Preps Quantity
