Dried Blood Spot (DBS) DNA Isolation Kit (Cat. 36000)
Dried Blood Spot (DBS) DNA Isolation Kit
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Dried Blood Spot (DBS) DNA Isolation Kit
Rapid preparation of total DNA from dried blood spots
- High quality DNA, free from RNA contamination
- Isolate genomic DNA from anticoagulated and untreated blood
- Rapid and convenient spin column procedure
- Isolate DNA from inputs as low as 20 µL
For research use only and NOT intended for in vitro diagnostics.
Norgen's DBS DNA Isolation Kit is a seamless alternative for Norgen's discontinued Blood DNA kit (52100).
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(Cat. 36000) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Blood Input
|
3 x 3 mm diameter punches
|
|
Average yield* (ng)
|
150 ng
|
|
Column Binding Capacity
|
> 50 µg
|
|
Time to Complete 10 Purifications
|
35 minutes
|
* Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Supporting Data
| Component | Cat. 36000 (50 preps) |
|---|---|
| Digestion Buffer B | 6 mL |
| Lysis Buffer B | 20 mL |
| Wash Solution WN | 18 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 15 mL |
| Proteinase K | 1.2 mL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes | 50 |
| Product Insert | 1 |
| Title | Molecular Survey of Dirofilaria and Leishmania Species in Dogs from Central Balkan |
| Journal | Animals. 2022. |
| Authors | Suzana Tasić-Otašević, Sara Savić, Maja Jurhar-Pavlova, Jovana Stefanovska, Marko Stalević, Aleksandra Ignjatović, Marina Ranđelović, Bojan Gajić, Aleksandar Cvetkovikj and Simona Gabrielli |
| Title | Evaluation of Dried Blood Spot Samples for Hepatitis C Virus Detection and Quantification |
| Journal | Journal of Clinical Virology. 2016. |
| Authors | Marques BL, do Espírito-Santo MP, Marques VA, Miguel JC, da Silva EF, Villela-Nogueira CA, Lewis-Ximenez LL, Lampe E, Villar LM. |
| Title | Human Babesiosis, Bolivia, 2013 |
| Journal | Emerging Infectious Diseases. 2016. |
| Authors | Simona Gabrielli, Valentina Totino, Fabio Macchioni, Freddy Zuñiga, Patricia Rojas, Yuni Lara, Mimmo Roselli, Alessandro BartoloniComments to Author , and Gabriella Cancrini |
| Title | Malaria in children of Tshimbulu (Western Kasai, Democratic Republic of the Congo): epidemiological data and accuracy of diagnostic assays applied in a limited resource setting |
| Journal | Malaria Journal. 2016. |
| Authors | Gabrielli S, Bellina L, Milardi GL, Katende BK, Totino V, Fullin V, Cancrini G |
Dried Blood Spot (DBS) DNA Isolation Kit
This kit is designed for the rapid preparation of total DNA from dried blood spots with a quick and convenient spin column. The Dried Blood Spot DNA Isolation Kit allows for the isolation of DNA from the blood of various species, including humans. Preparation time for a single sample is 30 minutes. The purified DNA is of high quality and is completely compatible with downstream applications including PCR, qPCR and more.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Blood Input
|
3 x 3 mm diameter punches
|
|
Average yield* (ng)
|
150 ng
|
|
Column Binding Capacity
|
> 50 µg
|
|
Time to Complete 10 Purifications
|
35 minutes
|
* Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 36000 (50 preps) |
|---|---|
| Digestion Buffer B | 6 mL |
| Lysis Buffer B | 20 mL |
| Wash Solution WN | 18 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 15 mL |
| Proteinase K | 1.2 mL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes | 50 |
| Product Insert | 1 |
| Title | Molecular Survey of Dirofilaria and Leishmania Species in Dogs from Central Balkan |
| Journal | Animals. 2022. |
| Authors | Suzana Tasić-Otašević, Sara Savić, Maja Jurhar-Pavlova, Jovana Stefanovska, Marko Stalević, Aleksandra Ignjatović, Marina Ranđelović, Bojan Gajić, Aleksandar Cvetkovikj and Simona Gabrielli |
| Title | Evaluation of Dried Blood Spot Samples for Hepatitis C Virus Detection and Quantification |
| Journal | Journal of Clinical Virology. 2016. |
| Authors | Marques BL, do Espírito-Santo MP, Marques VA, Miguel JC, da Silva EF, Villela-Nogueira CA, Lewis-Ximenez LL, Lampe E, Villar LM. |
| Title | Human Babesiosis, Bolivia, 2013 |
| Journal | Emerging Infectious Diseases. 2016. |
| Authors | Simona Gabrielli, Valentina Totino, Fabio Macchioni, Freddy Zuñiga, Patricia Rojas, Yuni Lara, Mimmo Roselli, Alessandro BartoloniComments to Author , and Gabriella Cancrini |
| Title | Malaria in children of Tshimbulu (Western Kasai, Democratic Republic of the Congo): epidemiological data and accuracy of diagnostic assays applied in a limited resource setting |
| Journal | Malaria Journal. 2016. |
| Authors | Gabrielli S, Bellina L, Milardi GL, Katende BK, Totino V, Fullin V, Cancrini G |
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