Dried Blood Spot (DBS) DNA Isolation Kit

Cat. 36000
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Dried Blood Spot (DBS) DNA Isolation Kit

Cat. 36000

Rapid preparation of total DNA from dried blood spots

  • High quality DNA, free from RNA contamination
  • Isolate genomic DNA from anticoagulated and untreated blood
  • Rapid and convenient spin column procedure
  • Isolate DNA from inputs as low as 20 µL

For research use only and NOT intended for in vitro diagnostics.

Norgen's DBS DNA Isolation Kit is a seamless alternative for Norgen's discontinued Blood DNA kit (52100).

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Dried Blood Spot (DBS) DNA Isolation Kit
(Cat. 36000)
50 preps

Kit Size

  • High quality DNA, free from RNA contamination
  • Isolate genomic DNA from anticoagulated and untreated blood
  • Rapid and convenient spin column procedure
  • Isolate DNA from inputs as low as 20 µL

Dried Blood Spot (DBS) DNA Isolation Kit

This kit is designed for the rapid preparation of total DNA from dried blood spots with a quick and convenient spin column.  The Dried Blood Spot DNA Isolation Kit allows for the isolation of DNA from the blood of various species, including humans. Preparation time for a single sample is 30 minutes.  The purified DNA is of high quality and is completely compatible with downstream applications including PCR, qPCR and more.

 

 

Kit Specifications

You have selected: Cat. 36000
Kit Specifications
Maximum Blood Input
3 x 3 mm diameter punches
Average yield* (ng)
150 ng
Column Binding Capacity
> 50 µg
Time to Complete 10 Purifications
35 minutes

 * Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Supporting Data

Click for expanded view

Component Cat. 36000 (50 preps)
Digestion Buffer B 6 mL
Lysis Buffer B 20 mL
Wash Solution WN 18 mL
Wash Solution A 18 mL
Elution Buffer B 15 mL
Proteinase K 1.2 mL
Micro Spin Columns 50
Collection Tubes 50
Elution Tubes 50
Product Insert 1

Title Molecular Survey of Dirofilaria and Leishmania Species in Dogs from Central Balkan
Journal Animals. 2022.
Authors Suzana Tasić-Otašević, Sara Savić, Maja Jurhar-Pavlova, Jovana Stefanovska, Marko Stalević, Aleksandra Ignjatović, Marina Ranđelović, Bojan Gajić, Aleksandar Cvetkovikj and Simona Gabrielli
Title Evaluation of Dried Blood Spot Samples for Hepatitis C Virus Detection and Quantification
Journal Journal of Clinical Virology. 2016.
Authors Marques BL, do Espírito-Santo MP, Marques VA, Miguel JC, da Silva EF, Villela-Nogueira CA, Lewis-Ximenez LL, Lampe E, Villar LM.
Title Human Babesiosis, Bolivia, 2013
Journal Emerging Infectious Diseases. 2016.
Authors Simona Gabrielli, Valentina Totino, Fabio Macchioni, Freddy Zuñiga, Patricia Rojas, Yuni Lara, Mimmo Roselli, Alessandro BartoloniComments to Author , and Gabriella Cancrini
Title Malaria in children of Tshimbulu (Western Kasai, Democratic Republic of the Congo): epidemiological data and accuracy of diagnostic assays applied in a limited resource setting
Journal Malaria Journal. 2016.
Authors Gabrielli S, Bellina L, Milardi GL, Katende BK, Totino V, Fullin V, Cancrini G

Dried Blood Spot (DBS) DNA Isolation Kit

This kit is designed for the rapid preparation of total DNA from dried blood spots with a quick and convenient spin column.  The Dried Blood Spot DNA Isolation Kit allows for the isolation of DNA from the blood of various species, including humans. Preparation time for a single sample is 30 minutes.  The purified DNA is of high quality and is completely compatible with downstream applications including PCR, qPCR and more.

 

 

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 36000
Kit Specifications
Maximum Blood Input
3 x 3 mm diameter punches
Average yield* (ng)
150 ng
Column Binding Capacity
> 50 µg
Time to Complete 10 Purifications
35 minutes

 * Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component Cat. 36000 (50 preps)
Digestion Buffer B 6 mL
Lysis Buffer B 20 mL
Wash Solution WN 18 mL
Wash Solution A 18 mL
Elution Buffer B 15 mL
Proteinase K 1.2 mL
Micro Spin Columns 50
Collection Tubes 50
Elution Tubes 50
Product Insert 1

Title Molecular Survey of Dirofilaria and Leishmania Species in Dogs from Central Balkan
Journal Animals. 2022.
Authors Suzana Tasić-Otašević, Sara Savić, Maja Jurhar-Pavlova, Jovana Stefanovska, Marko Stalević, Aleksandra Ignjatović, Marina Ranđelović, Bojan Gajić, Aleksandar Cvetkovikj and Simona Gabrielli
Title Evaluation of Dried Blood Spot Samples for Hepatitis C Virus Detection and Quantification
Journal Journal of Clinical Virology. 2016.
Authors Marques BL, do Espírito-Santo MP, Marques VA, Miguel JC, da Silva EF, Villela-Nogueira CA, Lewis-Ximenez LL, Lampe E, Villar LM.
Title Human Babesiosis, Bolivia, 2013
Journal Emerging Infectious Diseases. 2016.
Authors Simona Gabrielli, Valentina Totino, Fabio Macchioni, Freddy Zuñiga, Patricia Rojas, Yuni Lara, Mimmo Roselli, Alessandro BartoloniComments to Author , and Gabriella Cancrini
Title Malaria in children of Tshimbulu (Western Kasai, Democratic Republic of the Congo): epidemiological data and accuracy of diagnostic assays applied in a limited resource setting
Journal Malaria Journal. 2016.
Authors Gabrielli S, Bellina L, Milardi GL, Katende BK, Totino V, Fullin V, Cancrini G

 


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