Dried Blood Spot (DBS) DNA Isolation Kit

Figure 2. Purified DNA can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 3 x 3 mm diameter circles per sample using Norgen's Dried Blood Spot DNA Isolation Kit. Next, 3 µL (green line) & 9 µL (blue line) of the DNA from each of the 150 µL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.