Name: Dried Blood Spot (DBS) DNA Isolation Kit
Brand: Norgen Biotek Corp.
SKU: 36000
Price: 169.000000 USD
Availability: InStock

Overview

High quality DNA, free from RNA contamination
Isolate genomic DNA from anticoagulated and untreated blood
Rapid and convenient spin column procedure
Isolate DNA from inputs as low as 20 µL

This kit is designed for the rapid preparation of total DNA from dried blood spots with a quick and convenient spin column. The Dried Blood Spot DNA Isolation Kit allows for the isolation of DNA from the blood of various species, including humans. Preparation time for a single sample is 30 minutes. The purified DNA is of high quality and is completely compatible with downstream applications including PCR, qPCR and more.

Details

Supporting Data

Figure 1. DNA Isolated from 3 x 3 mm Diameter Circles. Blood collected on EDTA was applied to Whatman's 903 Protein Saver Card and allowed to dry for 1 week. DNA was isolated from 3 x 3 mm diameter circles per sample using Norgen's Dried Blood spot DNA Isolation Kit. Following isolation, 15 µL from each 150 µL elution was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation Kit demonstrated a good DNA yield and integrity. The ladder corresponds to Norgen's UltraRanger 1kb DNA Ladder.

Figure 2. Purified DNA can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 3 x 3 mm diameter circles per sample using Norgen's Dried Blood Spot DNA Isolation Kit. Next, 3 µL (green line) & 9 µL (blue line) of the DNA from each of the 150 µL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.

Kit Specifications
Maximum Blood Input	3 x 3 mm diameter punches
Average yield* (ng)	150 ng
Column Binding Capacity	> 50 µg
Time to Complete 10 Purifications	35 minutes

* Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 36000 (50 preps)
Digestion Buffer B	6 mL
Lysis Buffer B	20 mL
Wash Solution WN	18 mL
Wash Solution A	18 mL
Elution Buffer B	15 mL
Proteinase K	1.2 mL
Micro Spin Columns	50
Collection Tubes	50
Elution Tubes	50
Product Insert	1

Documentation

FAQs

Spin Column

I noticed the column was clogged. What causes this?

Column clogging can result from the following:

Inefficient cell lysis.
Check Proteinase K activity. Also ensure that the correct volume of Lysis Buffer B was added to the blood sample.
Cell debris may be clogging the column.
When a high cell number is expected in the blood sample, ensure that the optional spin for 2 minutes at 5,000 rpm after the Proteinase K incubation is performed. Take the clean supernatant only for the next binding step.
The sample is too large.
Too many cells were applied to the column. Ensure that Proteinase K and Lysis Buffer B are proportionally added as the blood volume is increased. Clogging can be alleviated by centrifuging for a longer period of time until the lysate passes through the column.

Why do I have a low genomic DNA yield?

A low genomic DNA yield may be caused by inefficient cell lysis. Ensure that the correct volume of Lysis Buffer B was added to the blood sample. Also increase incubation time up to 25-35 minutes at 56°C. Additionally, ensure that the correct volume of ethanol was added to the lysate before column binding.

Why is the purified DNA not performing well in downstream applications?

If the DNA does not perform well in downstream applications, It may be due to one or more of the following:

DNA was not washed three times with the provided solutions.
Ensure the column was washed one time with Solution WN and two times with Wash Solution A.
Ethanol carryover.
Ensure that the dry spin under the Column Wash procedure is performed, in order to remove traces of ethanol prior to elution. Ethanol is known to interfere with many downstream applications.
High DNA input used in PCR reaction.
For best results, make sure that the final concentration of DNA in the PCR reaction does not exceed 75 ng/μL (1.5 μg DNA per 20 μL PCR reaction)

I lost / finished the proteinase K in storage buffer from my Norgen Biotek kit. Can I purchase it separately?

Yes, Product number 28229 is the correct replacement part. It comes with 5 tubes of 1 mL each.

Citations

Title	Molecular Survey of Dirofilaria and Leishmania Species in Dogs from Central Balkan
Journal	Animals. 2022.
Authors	Suzana Tasić-Otašević, Sara Savić, Maja Jurhar-Pavlova, Jovana Stefanovska, Marko Stalević, Aleksandra Ignjatović, Marina Ranđelović, Bojan Gajić, Aleksandar Cvetkovikj and Simona Gabrielli

Title	Evaluation of Dried Blood Spot Samples for Hepatitis C Virus Detection and Quantification
Journal	Journal of Clinical Virology. 2016.
Authors	Marques BL, do Espírito-Santo MP, Marques VA, Miguel JC, da Silva EF, Villela-Nogueira CA, Lewis-Ximenez LL, Lampe E, Villar LM.

Title	Human Babesiosis, Bolivia, 2013
Journal	Emerging Infectious Diseases. 2016.
Authors	Simona Gabrielli, Valentina Totino, Fabio Macchioni, Freddy Zuñiga, Patricia Rojas, Yuni Lara, Mimmo Roselli, Alessandro BartoloniComments to Author , and Gabriella Cancrini

Title	Malaria in children of Tshimbulu (Western Kasai, Democratic Republic of the Congo): epidemiological data and accuracy of diagnostic assays applied in a limited resource setting
Journal	Malaria Journal. 2016.
Authors	Gabrielli S, Bellina L, Milardi GL, Katende BK, Totino V, Fullin V, Cancrini G

Dried Blood Spot (DBS) DNA Isolation Kit