Escherichia coli (E.coli) O157:H7 Quantified Bacterial DNA Standards (Cat. 28302)

Escherichia coli (E.coli) O157:H7 Quantified Bacterial DNA Standards

Cat. 28302
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Escherichia coli (E.coli) O157:H7 Quantified Bacterial DNA Standards

Cat. 28302

To be used as a positive control or PCR quantification standard for E. coli O157:H7.

  • Quantified standard to be used as a positive control or PCR quantification standard
  • Vigorously quantified using multiple methods

 

For research use only and NOT intended for in vitro diagnostics.

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E. coli O157:H7 Quantified Bacterial DNA Standards
(Cat. 28302) 250 μL

Product Size

Kit Specifications

You have selected: Cat. 28302

Volume Provided

250 μL (for STX1)

250 μL (for STX2)

DNA Quantity

2 x 104 copies per μL

 

Storage Conditions
Upon receipt, store Norgen’s E. coli O157:H7 Quantified Bacterial DNA Standards at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.

Supporting Data

Click for expanded view

Escherichia coli (E.coli) O157:H7 Quantified Bacterial DNA Standards

E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.

Norgen’s E. coli O157:H7 Quantified Bacterial DNA Standards are cloned fragments of the two Shiga-like toxin regions purified using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as positive controls or PCR quantification standards for E. coli O157:H7.
 

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 28302

Volume Provided

250 μL (for STX1)

250 μL (for STX2)

DNA Quantity

2 x 104 copies per μL

 

Storage Conditions
Upon receipt, store Norgen’s E. coli O157:H7 Quantified Bacterial DNA Standards at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.