EXTRAClean Urine Cell-Free Circulating RNA Purification Kits
A reliable, and simple procedure for isolating circulating RNA and exosomal RNA from various urine inputs volumes.
For research use only and NOT intended for in vitro diagnostics.
EXTRAClean Urine Cell-Free Circulating RNA Purification Kits
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Enhance your sequencing reads.
A reliable, and simple procedure for isolating circulating RNA and exosomal RNA from various urine inputs volumes.
Overview
- Versatile urine input ranges
- No phenol extractions
- Concentrate circulating RNA and exosomal RNA into flexible elution volumes
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS Services
- EXTRAClean resin separation matrix provides highly purified RNA.
Norgen's EXTRAClean Urine Cell-Free Circulating RNA Purification Kits provide a fast, reliable, reproducible, and simple procedure for isolating circulating RNA and exosomal RNA from various urine inputs ranging from 250 μL and up to 30 mL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extensions, and expression array assays.
EXTRAClean Cell-Free Circulating RNA Purification Mini Kit:
For sample volumes ranging from 250 μL to 2 mL
EXTRAClean Cell-Free Circulating RNA Purification Midi Kit:
For sample volumes ranging from 2mL to 10mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL
EXTRAClean Cell-Free Circulating RNA Purification Maxi Kit:
For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
Details
Supporting Data
Kit Specifications | |
---|---|
Sample Type | Fresh or frozen urine |
Sample Volume Range | 250 μL to 2mL |
Minimum Elution Volume | 50 μL |
Maximum Elution Volume | 100 μL |
Time to Complete 10 Purifications | 25–30 minutes |
Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
Average Yields ¥ | Variable depending on specimen |
¥Please check page 5 of protocol for average urine yields and common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Component | Mini (Cat. 72900) | Midi (Cat. 72910) | Maxi (Cat. 72920) |
---|---|---|---|
Binding Solution K | 25 mL | 75 mL | 1 x 75 mL 1 x 25 mL |
Lysis Buffer A | 30 mL | 20 mL | 20 mL |
Wash Solution A | 18 mL | 18 mL | 18 mL |
Elution Solution A | 6 mL | 6 mL | 6 mL |
Collection Tubes | 50 | 20 | 10 |
Elution Tubes (1.7 mL) | 50 | 20 | 10 |
EXTRAClean Mini Spin Columns | 50 | 20 | 10 |
EXTRAClean Midi Spin Columns | - | 20 | - |
EXTRAClean Maxi Spin Columns | - | - | 10 |
Product Insert | 1 | 1 | 1 |
Documentation
FAQs
Mini, Midi, Maxi
A fixed speed centrifuge can be used, however reduced yields may be observed.
All centrifugation steps are performed at room temperature. Centrifugation at 4°C will not adversely affect kit performance.
Adding more or less than the specified volumes may reduce both the quality and the quantity of the purified RNA. Eluting your RNA in high volumes will increase the yield but will lower the concentration. Eluting in small volumes will increase the concentration but will lower the overall yield.
Your purified RNA will be contaminated with the Wash Solution A. This may reduce the quality of your purified RNA and will interfere with your downstream applications.
Yes, but it is recommended that the 2nd elution be in a smaller volume (50% of 1st Elution). It is also recommended to perform the 2nd elution into a separate elution tube to avoid diluting the 1st elution.
Urine samples contain very little RNA. This varies from individual to individual based on numerous variables. In order to increase the yield, the amount of urine input could be increased.
Most of the Free-Circulating Urine RNA is short RNA fragments. The A260:280 ratio is normally between 1—1.6. This low A260:280 ratio will not affect any downstream application.
If a different Elution Buffer was used other than the one provided in the kit, the buffer should be checked for any components that may interfere with the application. Common components that are known to interfere are high salts (including EDTA), detergents and other denaturants. Check the compatibility of your elution buffer with the intended use.