FBS Exosome Depletion Kits All-in-one system for the depletion of cow's exosomes from FBS prior to using it as a growth supplement in your culture medium For research use only and NOT intended for in vitro diagnostics. Powered by Bioz FBS Exosome Depletion Kits All-in-one system for the depletion of cow's exosomes from FBS prior to using it as a growth supplement in your culture medium SKU 61100 Format Slurry Format Column Format Size 6 Preps 12 Preps Contact Distributor Overview Details Documentation Citations Overview Efficient depletion of cow's exosomes from Fetal Bovine Serum Deplete exosome-sized vesicles from versatile FBS volumes No protease treatment required No time-consuming ultracentrifugation No precipitation reagents required No overnight incubation required Depleted FBS has no detectable cow’s miRNA The depleted FBS provides the same cellular growth rates as the standard FBS Purification is based on Norgen’s proprietary Silicon Carbide resin matrix Norgen's FBS Exosome Depletion Kits constitute an all-in-one system for the depletion of cow's exosomes from FBS prior to using it as a growth supplement in your culture medium. The FBS recovered from the depletion process is exosome-depleted and does not contain any quantifiable bovine miRNAs. Moreover, the exosome-depleted FBS will support the growth of your cells of interest similar to the non-depleted FBS. Norgen's kits allows for the depletion of different FBS volumes. The depletion is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require ultracentrifugation, any special instrumentation, precipitation reagents or any protease treatments. More importantly, the depletion process is an inexpensive method for depletion of your own FBS, as compared to the current ready-to-use exosome-depleted media available on the market. Background Most culture medium used for the growth and propagation of cells in culture require the addition of fetal bovine serum (FBS) as a growth complement to media. FBS is obtained from bovine (cow) serum, and therefore contains large quantities of cow exosome vesicles. These exosomes may interfere with some types of studies, or may lead to unreliable results when studying the exosomes shed from your cells of interest in normal culture conditions. Therefore, the use of exosome-depleted FBS is highly recommended for many types of studies. FBS Exosome Depletion Kit (Slurry Format) For FBS volumes ranging from 140 mL to 280 mL. FBS Exosome Depletion Kit (Column Format) For FBS volumes ranging from 120 mL to 240 mL. Details Supporting Data Previous Figure 1. Exosome-depleted FBS with Norgen's FBS Exosome Depletion Kits (Slurry Format) has undetectable Bovine miRNA levels. Norgen's FBS Exosome Depletion Kit I (Slurry Format) (Cat# 61100) was used to deplete bovine miRNA from 5mL FBS. Total RNA/miRNA including exosomal RNA was purified from the depleted FBS, non-depleted FBS and a commercially available ready – to – go depleted FBS using Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit (Cat# 55800). Five different bovine microRNAs were assessed by RT-qPCR (miR-26a, miR-30a, miR-92a, miR-23a and miR-122). Three out of the five tested miRNA (miR-26a, miR-92a and miR-23a) didn’t show any amplification in the FBS depleted using Norgen's FBS Exosome Depletion Kit I (Slurry Format) whereas the other two miRNAs (miR-30a and miR-122) showed very late Ct. values which appeared to be a primer dimer according to the melt curve. Figure 2. Growth rates of HeLa cells in media containing Exosome-depleted FBS. Growth rates of HeLa cells in media containing Exosome-depleted FBS using Norgen's FBS Exosome Depletion Kits (Slurry Format) was compared to that in media containing standard FBS. Simply, HeLa cells were seeded in DMEM with either 10% Exosome-depleted FBS using Norgen's Kits or 10% standard FBS and then cultured under standard conditions at 37°C with 5% CO2 for 3 days. The cells were imaged using Moticam 480 to observe cellular morphology and growth rate. Similar growth and identical cellular morphology were detected for both the Exosome-depleted FBS using Norgen’s FBS Exosome Depletion Kits and the standard FBS Figure 3. Exosome-depleted FBS with Norgen's FBS Exosome Depletion Kits (Column Format) has undetectable Bovine miRNA levels. Norgen's FBS Exosome Depletion Kit I (Column Format) (Cat# 61200) was used to deplete bovine miRNA from 5mL FBS. Total RNA/miRNA including exosomal RNA was purified from the depleted FBS, non-depleted FBS and a commercially available ready – to – go depleted FBS using Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit (Cat# 55800). Five different bovine microRNAs were assessed by RT-qPCR (miR-26a, miR-30a, miR-92a, miR-23a and miR-122). Three out of the five tested miRNA (miR-26a, miR-92a and miR-23a) didn’t show any amplification in the FBS depleted using Norgen's FBS Exosome Depletion Kit I (Column Format) whereas the other two miRNAs (miR-30a and miR-122) showed very late Ct. values which appeared to be a primer dimer according to the melt curve. Next Click for expanded view Kit Specifications Sample Type Fetal Bovine Serum Sample Volume Range Up to 140 mL (FBS Exosome Depletion Kit I (Slurry Format) Up to 280 mL (FBS Exosome Depletion Kit II (Slurry Format) Depletion Deplete exosome-sized vesicle Bovine miRNA No detectable bovine miRNA Time to Complete 6 Purifications 40 minutes Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 61100 (6 preps) Cat. 61400 (12 preps) Cat. 61200 (6 preps) Cat. 61300 (12 preps) ExoC Buffer 2 x 1.5 mL 8 mL 2 x 1.5 mL 8 mL Slurry E 12.5 mL 2 x 12.5 mL - - Maxi Spin Column - - 6 12 Product Insert 1 1 1 1 Documentation PROTOCOLS (61100) FBS Exosome Depletion Kit I & Kit II (Slurry Format) - Protocol (6 prep) (61400) FBS Exosome Depletion Kit I & Kit II (Slurry Format) - Protocol (12 prep) (61200) FBS Exosome Depletion Kit I & Kit II (Column Format) - Protocol (6 prep) (61300) FBS Exosome Depletion Kit I & Kit II (Column Format) - Protocol (12 prep) SAFETY DATA SHEETS (61100, 61400) FBS Exosome Depletion Kit I & Kit II (Slurry Format) - Safety Data Sheet (61200, 61300) FBS Exosome Depletion Kit I & Kit II (Column Format) - Safety Data Sheet FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Powered by Bioz See more details on Bioz Related Products Exosomal RNA Isolation Kit
FBS Exosome Depletion Kits All-in-one system for the depletion of cow's exosomes from FBS prior to using it as a growth supplement in your culture medium For research use only and NOT intended for in vitro diagnostics. Powered by Bioz FBS Exosome Depletion Kits All-in-one system for the depletion of cow's exosomes from FBS prior to using it as a growth supplement in your culture medium SKU 61100 Format Slurry Format Column Format Size 6 Preps 12 Preps Contact Distributor