Food DNA Isolation Kit For the isolation of DNA directly from food or from enriched microorganisms For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Food DNA Isolation Kit For the isolation of DNA directly from food or from enriched microorganisms Price USD$169.00 SKU 54500 Format Spin Column Size 50 Preps Quantity Overview Details Documentation Citations Overview Isolate DNA from a wide range of food materials. (e.g boiled, fluid, processed or raw food products) No hazardous chemicals required (e.g. phenol or chloroform) Effective lysis with Proteinase K and optional lysozyme treatment Fast (less than 15 minutes hands-on time) and convenient processing using a rapid spin-column format Wide compatibility with a variety of food products for GMO-DNA isolation Universal protocol for food related pathogen DNA isolation (Gram positive and Gram negative) This kit provides a rapid spin column method for the isolation and purification of total DNA from a wide range of food samples originating from animals or plants. The kit is designed for identification of GMO-DNA or animal components in food and feed and can be used for a wide range of starting materials including raw or processed food, meat, liquids, sauces and dairy products including milk, cheese and yogurt. This kit also provides a convenient method for the detection of food-related pathogens and will isolate such DNA (enriched or as is) including Gram-positive bacteria, Gram-negative bacteria, yeast and fungi which may contaminate food sources. A number of pathogens have been tested including E. coli O157:H7, Staphylococcus, Listeria monocytogenes, Salmonella enterica & Campylobacter jejuni. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR based detection, sequencing and genotyping. Details Supporting Data Previous Figure 1. Isolate DNA from a Wide Range of Food Samples. Total genomic DNA was isolated from 200 mg of processed (chicken soup, beef jerky) or non-processed (raw pork, olive dressing, ground beef) food materials using Norgen's Food DNA Isolation kit. Following isolation, 10 µL from each 100 µL elution was loaded on 1% TAE agarose gel. Lane M: Norgen's HighRanger 1kb DNA Ladder. Figure 2. High Purity of DNA Samples. DNA was isolated from 200 mg of processed (chicken soup, beef jerky) or non-processed (raw pork, olive dressing, ground beef) food materials using Norgen's Food DNA Isolation Kit. DNA purity was then determined using a NanoDrop. All the DNA isolated from the different food samples showed a high quality (260/280=1.9-2.1). Figure 3. High Quality DNA free from PCR Inhibitors - Superior Quality to detect two house keeping genes (5S rDNA and 16S rDNA) representing GMO gene and food related pathogen detection. Total DNA was isolated from 200 mg samples of different food materials (processed and non-processed) using Norgen's Food DNA Isolation Kit. Next, 4 µL of the elution was then used as the template in 20 µL PCR reactions using universal 5s rDNA primers (A) and prokaryotic 16s rDNA primers (B) in real-time PCR (SYBR Green). Norgen's DNA was successfully amplified, indicating the high quality of the inhibitor-free DNA for GMO and pathogen detection from diversified food materials. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 50 μg Maximum Column Loading Volume 650 μL Maximum Amount of Starting Material: Solid food material Liquid sample (e.g. milk or concentrated juice) 200 mg 1 mL to 1.5 mL Time to Complete 10 Purifications 45 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature. Samples Tested by PCR Food Materials Samples that Have Been Tested by PCR Plant related Cereal, Jam, Chocolate, Spices, Sauce Animal related Raw and processed meat products (e.g. ham, beef jerky, taco seasoned ground beef, pork and sausage) Dairy product Milk, Yogurt, Cheese Pathogens (enriched from food samples) E. coli O157:H7 from food sample Staphylococcus from milk Listeria monocytogenes from milk Salmonella enterica from raw meat Campylobacter jejuni from milk Component Cat. 54500 (50 preps) Lysis Buffer L 60 mL Binding Buffer I 7 mL Buffer SK 30 mL Wash Solution A 18 mL Elution Buffer B 8 mL Proteinase K 1 vial Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (54500) Food DNA Isolation Kit - Protocol (50 prep) SAFETY DATA SHEETS (54500) Food DNA Isolation Kit - Safety Data Sheet FLYERS Flyer Related Products Genomic DNA Isolation Kit
Food DNA Isolation Kit For the isolation of DNA directly from food or from enriched microorganisms For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Food DNA Isolation Kit For the isolation of DNA directly from food or from enriched microorganisms Price USD$169.00 SKU 54500 Format Spin Column Size 50 Preps Quantity
