Food DNA Isolation Kit (Cat. 54500)
Food DNA Isolation Kit
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Food DNA Isolation Kit
For the isolation of DNA directly from food or from enriched microorganisms
- Isolate DNA from a wide range of food materials. (e.g boiled, fluid, processed or raw food products)
- No hazardous chemicals required (e.g. phenol or chloroform)
- Effective lysis with Proteinase K and optional lysozyme treatment
- Fast (less than 15 minutes hands-on time) and convenient processing using a rapid spin-column format
- Wide compatibility with a variety of food products for GMO-DNA isolation
- Universal protocol for food related pathogen DNA isolation (Gram positive and Gram negative)
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 54500) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity
|
50 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
| Maximum Amount of Starting Material: Solid food material Liquid sample (e.g. milk or concentrated juice) |
200 mg 1 mL to 1.5 mL |
| Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Samples Tested by PCR
| Food Materials | Samples that Have Been Tested by PCR |
|
Plant related
|
Cereal, Jam, Chocolate, Spices, Sauce |
|
Animal related
|
Raw and processed meat products (e.g. ham, beef jerky, taco seasoned ground beef, pork and sausage) |
|
Dairy product
|
Milk, Yogurt, Cheese |
|
Pathogens (enriched from food samples) |
E. coli O157:H7 from food sample Staphylococcus from milk Listeria monocytogenes from milk Salmonella enterica from raw meat Campylobacter jejuni from milk |
Supporting Data
| Component | Cat. 54500 (50 preps) |
|---|---|
| Lysis Buffer L | 60 mL |
| Binding Buffer I | 7 mL |
| Buffer SK | 30 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 8 mL |
| Proteinase K | 1 vial |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Food DNA Isolation Kit
This kit provides a rapid spin column method for the isolation and purification of total DNA from a wide range of food samples originating from animals or plants. The kit is designed for identification of GMO-DNA or animal components in food and feed and can be used for a wide range of starting materials including raw or processed food, meat, liquids, sauces and dairy products including milk, cheese and yogurt.
This kit also provides a convenient method for the detection of food-related pathogens and will isolate such DNA (enriched or as is) including Gram-positive bacteria, Gram-negative bacteria, yeast and fungi which may contaminate food sources.  A number of pathogens have been tested including E. coli O157:H7, Staphylococcus, Listeria monocytogenes, Salmonella enterica & Campylobacter jejuni.  The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR based detection, sequencing and genotyping. Â
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Supporting Data
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity
|
50 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
| Maximum Amount of Starting Material: Solid food material Liquid sample (e.g. milk or concentrated juice) |
200 mg 1 mL to 1.5 mL |
| Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Samples Tested by PCR
| Food Materials | Samples that Have Been Tested by PCR |
|
Plant related
|
Cereal, Jam, Chocolate, Spices, Sauce |
|
Animal related
|
Raw and processed meat products (e.g. ham, beef jerky, taco seasoned ground beef, pork and sausage) |
|
Dairy product
|
Milk, Yogurt, Cheese |
|
Pathogens (enriched from food samples) |
E. coli O157:H7 from food sample Staphylococcus from milk Listeria monocytogenes from milk Salmonella enterica from raw meat Campylobacter jejuni from milk |
| Component | Cat. 54500 (50 preps) |
|---|---|
| Lysis Buffer L | 60 mL |
| Binding Buffer I | 7 mL |
| Buffer SK | 30 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 8 mL |
| Proteinase K | 1 vial |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
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