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For the isolation of DNA directly from food or from enriched microorganisms
For research use only and NOT intended for in vitro diagnostics.
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Kit Specifications
Kit Specifications
|
|
Maximum Column Binding Capacity
|
50 μg
|
Maximum Column Loading Volume
|
650 μL
|
Maximum Amount of Starting Material: Solid food material Liquid sample (e.g. milk or concentrated juice) |
200 mg 1 mL to 1.5 mL |
Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Samples Tested by PCR
Food Materials | Samples that Have Been Tested by PCR |
Plant related
|
Cereal, Jam, Chocolate, Spices, Sauce |
Animal related
|
Raw and processed meat products (e.g. ham, beef jerky, taco seasoned ground beef, pork and sausage) |
Dairy product
|
Milk, Yogurt, Cheese |
Pathogens (enriched from food samples) |
E. coli O157:H7 from food sample Staphylococcus from milk Listeria monocytogenes from milk Salmonella enterica from raw meat Campylobacter jejuni from milk |
Supporting Data
Food DNA Isolation Kit
This kit provides a rapid spin column method for the isolation and purification of total DNA from a wide range of food samples originating from animals or plants. The kit is designed for identification of GMO-DNA or animal components in food and feed and can be used for a wide range of starting materials including raw or processed food, meat, liquids, sauces and dairy products including milk, cheese and yogurt.
This kit also provides a convenient method for the detection of food-related pathogens and will isolate such DNA (enriched or as is) including Gram-positive bacteria, Gram-negative bacteria, yeast and fungi which may contaminate food sources.  A number of pathogens have been tested including E. coli O157:H7, Staphylococcus, Listeria monocytogenes, Salmonella enterica & Campylobacter jejuni.  The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR based detection, sequencing and genotyping. Â
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Supporting Data
Kit Specifications
Kit Specifications
|
|
Maximum Column Binding Capacity
|
50 μg
|
Maximum Column Loading Volume
|
650 μL
|
Maximum Amount of Starting Material: Solid food material Liquid sample (e.g. milk or concentrated juice) |
200 mg 1 mL to 1.5 mL |
Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Samples Tested by PCR
Food Materials | Samples that Have Been Tested by PCR |
Plant related
|
Cereal, Jam, Chocolate, Spices, Sauce |
Animal related
|
Raw and processed meat products (e.g. ham, beef jerky, taco seasoned ground beef, pork and sausage) |
Dairy product
|
Milk, Yogurt, Cheese |
Pathogens (enriched from food samples) |
E. coli O157:H7 from food sample Staphylococcus from milk Listeria monocytogenes from milk Salmonella enterica from raw meat Campylobacter jejuni from milk |