Grapevine Red Blotch-Associated Virus (GRBaV) TaqMan PCR Detection Kits

Supporting Data

Figure 1. Grape leaf, petiole and cane samples from known Grapevine red blotch-associated virus (GRBaV) infected vines were processed by Norgen’s Plant DNA Isolation Kit for GRBaV detection. The real-time PCR result (A) indicates a successful GRBaV detection from all different grape sample types and PCR internal control (B) indicates the high quality of DNA isolated that shows no PCR inhibition.

Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM65850 (100 preps)	Cat. TM65810 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	-
GRBaV Primer & Probe Mix	280 μL	280 μL
GRBaV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1