Overview

Norgen’s HPV Low Risk (6/11) TaqMan PCR Kit is designed for the detection of HPV Low Risk (6/11) specific DNA in a real-time PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of HPV Low Risk (6/11) specific DNA is based on TaqMan PCR providing a simple, reliable and rapid result for the detection of HPV Low Risk (6/11) infection. Norgen’s HPV Low Risk (6/11) TaqMan PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The HPV Low Risk (6/11) TaqMan PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents. The HPV Low Risk (6/11) TaqMan PCR Kit distinguishes between the detection of HPV 6 and HPV 11, allowing for clear and accurate results. HPV 11 detection is shown by amplification in the FAM channel. HPV 6 detection is shown by amplification in the Cy5 channel.

Norgen’s HPV Low Risk (6/11) TaqMan PCR Kit was developed and validated to be used with the following PCR instruments:

• Qiagen Rotor-Gene Q
• BioRad CFX96 TouchTM Real-Time PCR Detection System
• QuantStudioTM 7 Pro Real-Time PCR System

Details

Supporting Data

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Figure 1. Example of TaqMan PCR Positive result. Both PCR signals (FAM and Cy5) above the baseline from FAM, Cy5 and HEX channel indicate the successful PCR.

Figure 2. Example of TaqMan PCR Positive (Cy5) result. Both PCR signals above the baseline from Cy5 and HEX channel indicate the successful PCR.

Figure 3. Example of TaqMan PCR Positive (FAM) result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

Figure 4. Example of TaqMan PCR Negative result. No target DNA was detected in FAM and Cy5 channel but amplification signal from HEX indicates the successful PCR.

Figure 5. Example of TaqMan PCR inhibition result. No signal from FAM, Cy5 and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification

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