Hop Stunt Viroid (HSVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Hop Stunt Viroid (HSVd) TaqMan RT-PCR Detection Kits Price USD$749.00 SKU TM38950 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for HSVd Available in TaqMan format for analysis The Hop Stunt Viroid (HSVd) is one of a group of the smallest self-replicating RNAs pathogenic to plants. The viroid is a covalently closed, circular, single-stranded RNA consisting of 297 nucleotides. Strains of HSVd can be found to infect citrus, grapes, stone fruit and cucumber, and have been detected in Europe, Asia, North America, Australia and the Middle East. To prevent or reduce the negative effects caused by the viroid it is necessary to use viroid-free planting material. Methods for the identification of this viroid in host plants should help to control the spread of this agriculturally important plant pathogen. TaqMan PCR Kit Click to expand options HSVd TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents HSVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM38950 (100 preps) Cat. TM38910 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - HSVd Primer & Probe Mix 280 μL 280 μL HSVd Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM38950) Hop Stunt Viroid (HSVd) TaqMan RT-PCR Kit - Protocol (100 rxns) (TM38910) HSVd TaqMan Probe/Primer and Control Set - Protocol (100 rxns) POSTERS Rapid Isolation and Sensitive Detection of the Chrysanthemum Chlorotic Mottle Viroid Citations Powered by Bioz See more details on Bioz Related Products HSVd TaqMan Probe/Primer and Control Set Plant/Fungi Total RNA Purification Kit
Hop Stunt Viroid (HSVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Hop Stunt Viroid (HSVd) TaqMan RT-PCR Detection Kits Price USD$749.00 SKU TM38950 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity
