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Listeria monocytogenes has emerged as a significant foodborne pathogen that poses a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium. Due to its ability to survive high and low temperatures as well as low pH, it could resist various food processing technologies, as well as grow at food storage temperatures. L. monocytogenes is known to be associated with raw meat, unpasteurized milk and dairy products, vegetables, and seafood. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals being the most susceptible.

Norgen’s Listeria monocytogenes Quantified Bacterial DNA Standard is prepared from cultured bacteria using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Listeria monocytogenes.
 

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Figure 1. Accurate Quantity and High Quality Listeria monocytogenes DNA Standard. Two micro-liters of Norgen’s Listeria monocytogenes Quantified Bacterial DNA Standard and various serial dilutions (blue curves) were used in a 20 µL Listeria monocytogenes-specific PCR reaction using Norgen’s 2X PCR Master Mix (#28007), supplemented with SYBR Green I. The real-time PCR reactions were performed on a Bio-Rad CFXReal-Time PCR System. No template controls with water were also performed (yellow curves). Norgen’s Listeria monocytogenes Quantified Bacterial DNA Standard and various serial dilutions showed consistent amplification down to fewer than 40 copies per reaction.

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