Low Abundance DNA Quantification Kit PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Low Abundance DNA Quantification Kit PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range Price USD$279.00 SKU 57200 Size 48 Reactions Quantity Overview Details Documentation Citations Overview Quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range Compatible with any Real-Time PCR system DNA is accurately quantified using a standard curve constructed from the provided DNA standard Norgen’s Low Abundance DNA Quantification Kit offers a PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range. The kit consists of a specially designed primer mix, that is used in conjunction with the provided 2x PCR Master Mix, to amplify human DNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown DNA is accurately quantified by using a standard curve constructed from the provided DNA Standard. Details Supporting Data Previous Figure 1. Sensitivity of DNA Quantification of the Low Abundance DNA Quantification Kit Compared to Other Methods. A diagram representing the dynamic range of different DNA quantification methods is presented here. The Low Abundance DNA Quantification Kit can quantify purified DNA from low abundance samples in the pg and sub-pg range. Figure 2. Sensitivity of DNA Quantification in the Picogram Range Using the Low Abundance DNA Quantification Kit. A representative qPCR Baseline Graph showing the amplification of a DNA standard dilution series. The Low Abundance DNA Quantification Kit can quantify purified DNA from low abundance samples such as liquid biopsies (Plasma or Urine). As little as 500 fg of DNA can be quantified. Next Click for expanded view Storage Conditions Upon receipt, store Norgen’s Low Abundance DNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycle. If needed, prepare smaller working aliquots and store at -20°C or lower. Component Cat. 57200 (48 reactions) 2X PCR Master Mix 1 mL DNA Quantification Primer Set Mix 200 µL Quantified DNA Standard 5 standards, each 100 µL Nuclease-Free Water 1.25 mL Product Insert 1 Documentation PROTOCOLS (57200) Low Abundance DNA Quantification Kit - Protocol (48 reactions) APPLICATION NOTES Comparing Different DNA and RNA Quantification Methods for Biological Samples with Low Nucleic Acid Abundance SAFETY DATA SHEETS (57200) Low Abundance DNA Quantification Kit - Safety Data Sheet FLYERS Flyer Related Products Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit Plasma/Serum RNA Purification Maxi Kit
Low Abundance DNA Quantification Kit PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Low Abundance DNA Quantification Kit PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range Price USD$279.00 SKU 57200 Size 48 Reactions Quantity
