Low Abundance RNA Quantification Kit PCR-based detection procedure to quantify RNA over a wide dynamic range of concentrations For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Low Abundance RNA Quantification Kit PCR-based detection procedure to quantify RNA over a wide dynamic range of concentrations Price USD$335.00 SKU 58900 Size 48 Reactions Quantity Overview Details Documentation Citations Overview Quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL rangeCompatible with any Real-Time PCR systemRNA is accurately quantified using a standard curve constructed from the provided RNA standard Norgen’s Low Abundance RNA Quantification Kit offers a PCR-based detection procedure to quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range. The kit has two main enzymatic components – reverse transcription using Norgen’s microScript Reverse Transcription system and PCR Master Mix used in conjunction with a specially formulized primer mixture, to amplify human RNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown RNA is accurately quantified by using a standard curve constructed from the provided RNA Standard. Details Supporting Data Previous Figure 1. Sensitivity of RNA Quantification with the Low Abundance RNA Quantification Kit compared to Other Methods. A diagram representing the dynamic range of different RNA quantification method is presented here. The Low Abundance RNA Quantification Kit can quantify purified RNA from low abundance samples in the pg and sub-pg range. Figure 2. A representative RT-qPCR baseline graph showing the successful amplification of a dilution series of Quantified RNA Standard. Figure 3. Sensitivity of RNA Quantification from Small Volumes of Human Plasma using the Low Abundance RNA Quantification Kit. A representative qPCR Baseline Graph showing the amplification of total plasma RNA isolated from either 50 or 200 µL of human plasma using Norgen's Plasma/Serum RNA Purification Mini Kit (Cat. 55000). The Low Abundance RNA Quantification Kit could quantify purified RNA from such low abundance samples (and others such as urine, exosomes etc) with purified RNA concentrations that are 100 pg per µL or less. Figure 4. Sensitive RNA Quantification from Small Volumes of Plasma Next Click for expanded view Storage Conditions Upon receipt, store Norgen’s Low Abundance RNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower. Component Cat. 58900 (48 reactions) microScript microRNA Enzyme Mix 20 μL 2x microScript Reverse Transcriptase Reaction Mix 0.5 mL 2X Real-Time PCR Master Mix 1 mL RNA Quantification Primer Set Mix 200 μL Quantified RNA Standard 50 pg/μL 2 x 60 μL Nuclease-Free Water 3 x 1.25 mL Product Insert 1 Documentation PROTOCOLS (58900) Low Abundance RNA Quantification Kit - Protocol (48 rxns) APPLICATION NOTES Comparing Different DNA and RNA Quantification Methods for Biological Samples with Low Nucleic Acid Abundance SAFETY DATA SHEETS (58900) Low Abundance RNA Quantification Kit - Safety Data Sheet FLYERS Flyer Citations Title DNA-PKcs promotes sepsis-induced multiple organ failure by triggering mitochondrial dysfunction Journal Journal of Advanced Research. 2022. Authors Rongjun Zou, Jun Tao, Junxiong Qiu, Huimin Lu, Jianhua Wu, Hang Zhu, Ruibing Li, David Mui, Sam Toan, Xing Chang, Hao Zhou, Xiaoping Fan Related Services NGS Related Products Plasma/Serum RNA Purification Kit Plasma/Serum Exosome Purification and RNA Isolation Mini Kit Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit Urine Cell-Free Circulating RNA Purification Mini Kit Urine Exosome Purification and RNA Isolation Mini Kit
Low Abundance RNA Quantification Kit PCR-based detection procedure to quantify RNA over a wide dynamic range of concentrations For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Low Abundance RNA Quantification Kit PCR-based detection procedure to quantify RNA over a wide dynamic range of concentrations Price USD$335.00 SKU 58900 Size 48 Reactions Quantity
