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PCR-based detection procedure to quantify RNA over a wide dynamic range of concentrations
For research use only and NOT intended for in vitro diagnostics.
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Kit Specifications
Storage Conditions
Upon receipt, store Norgen’s Low Abundance RNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
Supporting Data
Title | DNA-PKcs promotes sepsis-induced multiple organ failure by triggering mitochondrial dysfunction |
Journal | Journal of Advanced Research. 2022. |
Authors | Rongjun Zou, Jun Tao, Junxiong Qiu, Huimin Lu, Jianhua Wu, Hang Zhu, Ruibing Li, David Mui, Sam Toan, Xing Chang, Hao Zhou, Xiaoping Fan |
Low Abundance RNA Quantification Kit
Norgen’s Low Abundance RNA Quantification Kit offers a PCR-based detection procedure to quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range. The kit has two main enzymatic components – reverse transcription using Norgen’s microScript Reverse Transcription system and PCR Master Mix used in conjunction with a specially formulized primer mixture, to amplify human RNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown RNA is accurately quantified by using a standard curve constructed from the provided RNA Standard.
Supporting Data
Kit Specifications
Storage Conditions
Upon receipt, store Norgen’s Low Abundance RNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
Title | DNA-PKcs promotes sepsis-induced multiple organ failure by triggering mitochondrial dysfunction |
Journal | Journal of Advanced Research. 2022. |
Authors | Rongjun Zou, Jun Tao, Junxiong Qiu, Huimin Lu, Jianhua Wu, Hang Zhu, Ruibing Li, David Mui, Sam Toan, Xing Chang, Hao Zhou, Xiaoping Fan |