Overview

Norgen’s Low Abundance RNA Quantification Kit offers a PCR-based detection procedure to quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range. The kit has two main enzymatic components – reverse transcription using Norgen’s microScript Reverse Transcription system and PCR Master Mix used in conjunction with  a specially formulized primer mixture, to amplify human RNA from different types of inputs (such as various liquid biopsies). The kit contains a fluorescent DNA dye, and is compatible with any Real-Time PCR system with FAm/SYBR Green detection. The unknown RNA is accurately quantified by using a standard curve constructed from the provided RNA Standard. 

Details

Supporting Data

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Figure 1. Sensitivity of RNA Quantification with the Low Abundance RNA Quantification Kit compared to Other Methods. A diagram representing the dynamic range of different RNA quantification method is presented here.  The Low Abundance RNA Quantification Kit can quantify purified RNA from low abundance samples in the pg and sub-pg range.

Figure 2.   A representative RT-qPCR baseline graph showing the successful amplification of a dilution series of Quantified RNA Standard.

Figure 3. Sensitivity of RNA Quantification from Small Volumes of Human Plasma using the Low Abundance RNA Quantification Kit. A representative qPCR Baseline Graph showing the amplification of total plasma RNA isolated from either 50 or 200 µL of human plasma using Norgen's Plasma/Serum RNA Purification Mini Kit (Cat. 55000). The Low Abundance RNA Quantification Kit could quantify purified RNA from such low abundance samples (and others such as urine, exosomes etc) with purified RNA concentrations that are 100 pg per µL or less.

Figure 4. Sensitive RNA Quantification from Small Volumes of Plasma

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