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for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations
For research use only and NOT intended for in vitro diagnostics.
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Kit Specifications
Storage Conditions and Product Stability
This kit is stable for 1 year after the date of shipment .
Storage Conditions and Product Stability
Norgen's microScript microRNA cDNA Synthesis Kit components should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
Supporting Data
Title | A Method to Produce vsiRNAs in Plants with Cross-Kingdom Gene Silencing Capacity |
Journal | Appl. Sci.. 2022. |
Authors | Hernán de Jesús Villanueva-Alonzo, Ana Paulina Haro-Álvarez, Arturo A. Alvarado-Segura, Raúl Enrique Valle-Gough, Juan Gualberto Collí-Mull, Alberto Cal-Torres, Víctor Ermilo Arana-Argáez, Julio César Torres-Romero, Oscar Alberto Moreno-Valenzuela, Geovanny Nic-Can, Benjamín Abraham Ayil-Gutiérrez and Karla Y. Acosta-Viana |
Title | Association between the Expression Levels of MicroRNA-101, -103, and -29a with Autotaxin and Lysophosphatidic Acid Receptor 2 Expression in Gastric Cancer Patients |
Journal | Journal of Oncology. 2022. |
Authors | Sara Tutunchi, Saeedeh Akhavan, Ghodratollah Panahi, Mina Zare, Amirnader Emami Razavi, and Reza Shirkoohi |
Title | Moderate Endurance raining and MitoQ Improve Cardiovascular Function, Oxidative Stress, and Inflammation in Hypertensive Individuals: The Role of miR-21 and miR-222: A Randomized, Double- lind, Clinical Trial |
Journal | Cell Journal . 2022. |
Authors | Yaser Masoumi-Ardakani, M.Sc., Hamid Najafipour, Ph.D., Hamid Reza Nasri, M.D., Soheil Aminizadeh, Ph.D., Shirin Jafari, M.D., Zohreh Safi, B.Sc. |
Title | Effect of Combined Endurance Training and MitoQ on Cardiac Function and Serum Level of Antioxidants, NO, miR-126, and miR-27a in Hypertensive Individuals |
Journal | BioMed Research International. 2022. |
Authors | Yaser Masoumi-Ardakan, Hamid Najafipour, Hamid Reza Nasri, Soheil Aminizadeh, Shirin Jafari and Daruosh Moflehi |
Title | Association between the Expression Levels of MicroRNA-101, -103, and -29a with Autotaxin and Lysophosphatidic Acid Receptor 2 Expression in Gastric Cancer Patients. |
Journal | Journal of Oncology. 2022. |
Authors | Tutunchi S, Akhavan S, Panahi G, Zare M, Emami Razavi A, Shirkoohi R |
microScript microRNA cDNA Synthesis Kit
Norgen's microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix. The kit utilizes Norgen’s microScript Reverse Transcriptase, a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It has reduced RNase H activity and increased thermal stability.
The workflow of Norgen's microScript microRNA cDNA Synthesis Kit involves a simple, single-tube set-up by the mixing of 2x Reaction Mix, Enzyme Mix and the RNA template. The reaction can then be carried out in a thermocycler. A poly (A) tail is first added to the RNA template, followed by cDNA synthesis using an adapter primer. In addition to the ease-of-use, the single-tube set-up provides superb consistency and sensitivity. The cDNA could be used in a PCR or qPCR amplification using a Universal PCR Reverse Primer and the forward primer that contains the sequence of the microRNA of interest. A single cDNA preparation could be used for PCR amplification of a number of different microRNAs. In addition, the cDNA preparation could be used for PCR or qPCR detection (using gene-specific forward and reverse primers) of mRNA or large RNA if total RNA preparation was the starting template. This could allow for parallel evaluation of expression level of microRNAs and microRNA-targets.
Supporting Data
Kit Specifications
Storage Conditions and Product Stability
This kit is stable for 1 year after the date of shipment .
Storage Conditions and Product Stability
Norgen's microScript microRNA cDNA Synthesis Kit components should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
Title | A Method to Produce vsiRNAs in Plants with Cross-Kingdom Gene Silencing Capacity |
Journal | Appl. Sci.. 2022. |
Authors | Hernán de Jesús Villanueva-Alonzo, Ana Paulina Haro-Álvarez, Arturo A. Alvarado-Segura, Raúl Enrique Valle-Gough, Juan Gualberto Collí-Mull, Alberto Cal-Torres, Víctor Ermilo Arana-Argáez, Julio César Torres-Romero, Oscar Alberto Moreno-Valenzuela, Geovanny Nic-Can, Benjamín Abraham Ayil-Gutiérrez and Karla Y. Acosta-Viana |
Title | Association between the Expression Levels of MicroRNA-101, -103, and -29a with Autotaxin and Lysophosphatidic Acid Receptor 2 Expression in Gastric Cancer Patients |
Journal | Journal of Oncology. 2022. |
Authors | Sara Tutunchi, Saeedeh Akhavan, Ghodratollah Panahi, Mina Zare, Amirnader Emami Razavi, and Reza Shirkoohi |
Title | Moderate Endurance raining and MitoQ Improve Cardiovascular Function, Oxidative Stress, and Inflammation in Hypertensive Individuals: The Role of miR-21 and miR-222: A Randomized, Double- lind, Clinical Trial |
Journal | Cell Journal . 2022. |
Authors | Yaser Masoumi-Ardakani, M.Sc., Hamid Najafipour, Ph.D., Hamid Reza Nasri, M.D., Soheil Aminizadeh, Ph.D., Shirin Jafari, M.D., Zohreh Safi, B.Sc. |
Title | Effect of Combined Endurance Training and MitoQ on Cardiac Function and Serum Level of Antioxidants, NO, miR-126, and miR-27a in Hypertensive Individuals |
Journal | BioMed Research International. 2022. |
Authors | Yaser Masoumi-Ardakan, Hamid Najafipour, Hamid Reza Nasri, Soheil Aminizadeh, Shirin Jafari and Daruosh Moflehi |
Title | Association between the Expression Levels of MicroRNA-101, -103, and -29a with Autotaxin and Lysophosphatidic Acid Receptor 2 Expression in Gastric Cancer Patients. |
Journal | Journal of Oncology. 2022. |
Authors | Tutunchi S, Akhavan S, Panahi G, Zare M, Emami Razavi A, Shirkoohi R |