• Convenient
  • One cDNA Synthesis, Multiple microRNAs and microRNA-targets analyzed
  • Time Savings
  • Cost Efficient
  • High Sensitivity and Yield
  • Robust Enzyme
  • Available in 12 or 50 reaction size

ItemsCat. #Size
microScript microRNA cDNA Synthesis Kit 54410 50 Reactions
microScript microRNA cDNA Synthesis Kit 54415 12 Reactions

microScript microRNA cDNA Synthesis Kit

Norgen's microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix. The kit utilizes Norgen’s microScript Reverse Transcriptase, a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It has reduced RNase H activity and increased thermal stability.

The workflow of Norgen's microScript microRNA cDNA Synthesis Kit involves a simple, single-tube set-up by the mixing of 2x Reaction Mix, Enzyme Mix and the RNA template. The reaction can then be carried out in a thermocycler. A poly (A) tail is first added to the RNA template, followed by cDNA synthesis using an adapter primer. In addition to the ease-of-use, the single-tube set-up provides superb consistency and sensitivity. The cDNA could be used in a PCR or qPCR amplification using a Universal PCR Reverse Primer and the forward primer that contains the sequence of the microRNA of interest. A single cDNA preparation could be used for PCR amplification of a number of different microRNAs. In addition, the cDNA preparation could be used for PCR or qPCR detection (using gene-specific forward and reverse primers) of mRNA or large RNA if total RNA preparation was the starting template. This could allow for parallel evaluation of expression level of microRNAs and microRNA-targets.

Component Product # 54415
(12 Reactions)
Product # 54410
(50 Reactions)
microScript microRNA Enzyme Mix 12 μL 50 μL
2x Reaction Mix 120 μL 500 μL
Universal PCR Reverse Primer 60 μL 250 μL
Nuclease-Free Water 1.25 mL 2 x 1.25 mL
Product Insert 1 1

Storage Conditions and Product Stability
Norgen's microScript microRNA cDNA Synthesis Kit components should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.