Mycoplasma TaqMan PCR Detection Kits

Cat. TM33150, TM33110

Mycoplasma TaqMan PCR Detection Kits

Cat.

For research use only and NOT intended for in vitro diagnostics.

Want a discount?

Create your account to receive 15% off on your first purchase!

Mycoplasma TaqMan PCR Kit
(Cat. TM33150) 100 rxns

Mycoplasma TaqMan Probe/Primer and Control Set
(Cat. TM33110) 100 rxns

.product-table .captcha-admin-links, .product-table, #price-wrapper, #quant-cart-wrapper, .commerce-add-to-cart .quantity-wrapper, #noscript-product-table .input-group-addon, .javascript-ordering{ display: none; } .product-table#noscript-product-table{ display:grid; grid-template-columns:repeat(2, 1fr); } #noscript-product-table{ margin-bottom:0px; } #noscript-product-table .norshoppingtable{ margin:auto; }

Format: PCR
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Size:100 rxns

Overview

Mycoplasma TaqMan PCR Detection Kits

Detection kits for Mycoplasma DNA
Available in TaqMan format for analysis

Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.

Click to expand options

Mycoplasma TaqMan PCR Kit, 100 reactions

Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents

Mycoplasma TaqMan PCR Probe/Primer Set and Controls, 100 reactions

Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit -Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqManPCR Kit-Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

Kit Specifications

Cat.

Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Improve Your Workflow with Best-in-Class Total RNA Purification Technology | Learn More

Mycoplasma TaqMan PCR Detection Kits (Cat. TM33150, TM33110)

Mycoplasma TaqMan PCR Detection Kits

Mycoplasma TaqMan PCR Detection Kits

Format: PCR
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Size:100 rxns