Mycoplasma Detection Kits (Cat. TM33100, TM33110, EP33100)

Detection kits for Mycoplasma DNA
Available in 2 formats for analysis:

TaqMan Probe
Cat. TM33100 End-Point PCR
Cat. EP33100

Items	Cat. #	Size
Mycoplasma TaqMan PCR Kit	TM33100	24 rxns
Mycoplasma TaqMan Probe/Primer and Control Set	TM33110	100 rxns
Mycoplasma End-Point PCR Kit	EP33100	24 rxns
Mycoplasma Primer and Control Set	EP33110	100 rxns

Mycoplasma Detection Kits

Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.

There are 2 kits available for the detection of Mycoplasma:

Mycoplasma TaqMan PCR Kit

PCR control to monitor for PCR inhibition and validate the quality
Master Mix for the target and PCR control detection
Primer and Probe mix
Positive control and a negative control to confirm the integrity of the kit reagents

Mycoplasma End-Point PCR Kit

Master Mix and primers for the specific amplification of a 327 nucleotide region of the Mycoplasma genome
Positive control and a negative control to confirm the integrity of the kit reagents
Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.

Protocols:

TM33100 - Protocol (24 rxns)

TM33110 - Protocol (100 rxns)

EP33100 - Protocol (24 rxns)

EP33110 - Protocol (100 rxns)

Items	Cat. #	Size
Mycoplasma TaqMan PCR Kit	TM33100	24 rxns
Mycoplasma TaqMan Probe/Primer and Control Set	TM33110	100 rxns
Mycoplasma End-Point PCR Kit	EP33100	24 rxns
Mycoplasma Primer and Control Set	EP33110	100 rxns

Mycoplasma Detection Kits

Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.

There are 2 kits available for the detection of Mycoplasma:

Mycoplasma TaqMan PCR Kit

PCR control to monitor for PCR inhibition and validate the quality
Master Mix for the target and PCR control detection
Primer and Probe mix
Positive control and a negative control to confirm the integrity of the kit reagents

Mycoplasma End-Point PCR Kit

Master Mix and primers for the specific amplification of a 327 nucleotide region of the Mycoplasma genome
Positive control and a negative control to confirm the integrity of the kit reagents
Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.