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Mycoplasma Detection Kits (Cat. TM33100, TM33110, EP33100)

 

Purchasing Information

  • Detection kits for Mycoplasma DNA
  • Available in 2 formats for analysis:
TaqMan Probe
Cat. TM33100 End-Point PCR
Cat. EP33100
 

Mycoplasma TaqMan PCR Kit (Cat. TM33100) 24 rxns
Mycoplasma TaqMan Probe/Primer and Control Set (Cat. TM33110) 100 rxns
Mycoplasma End-Point PCR Kit (Cat. EP33100) 24 rxns
Mycoplasma Primer and Control Set (Cat. EP33110) 100 rxns

  • Detection kits for Mycoplasma DNA
  • Available in 2 formats for analysis:
TaqMan Probe
Cat. TM33100 End-Point PCR
Cat. EP33100
 


Mycoplasma Detection Kits

Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.

There are 2 kits available for the detection of Mycoplasma:


Click to expand options
Mycoplasma TaqMan PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents
Mycoplasma TaqMan PCR/RT-PCR Probe/Primer Set and Controls, 100 reactions
  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

Click to expand options
Mycoplasma End-Point PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer mix for the pathogen/virus/viroid of interest
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate qualitative analysis of the results on an agarose gel
Mycoplasma End-Point PCR/RT-PCR Primer Set and Controls, 100 reactions
  • Specific Primer mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


This product has a minimum of 10 per order.

Mycoplasma TaqMan PCR Kit (Cat. TM33100) 24 rxns
Mycoplasma TaqMan Probe/Primer and Control Set (Cat. TM33110) 100 rxns
Mycoplasma End-Point PCR Kit (Cat. EP33100) 24 rxns
Mycoplasma Primer and Control Set (Cat. EP33110) 100 rxns

  • Detection kits for Mycoplasma DNA
  • Available in 2 formats for analysis:
TaqMan Probe
Cat. TM33100 End-Point PCR
Cat. EP33100
 


Mycoplasma Detection Kits

Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.

There are 2 kits available for the detection of Mycoplasma:


Click to expand options
Mycoplasma TaqMan PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents
Mycoplasma TaqMan PCR/RT-PCR Probe/Primer Set and Controls, 100 reactions
  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

Click to expand options
Mycoplasma End-Point PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer mix for the pathogen/virus/viroid of interest
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate qualitative analysis of the results on an agarose gel
Mycoplasma End-Point PCR/RT-PCR Primer Set and Controls, 100 reactions
  • Specific Primer mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.

 

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