Mycoplasma TaqMan PCR Detection Kits (Cat. TM33150, TM33110)
Mycoplasma TaqMan PCR Detection Kits
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Mycoplasma TaqMan PCR Detection Kits
- Detection kits for Mycoplasma DNA
- Available in TaqMan format for analysis
For research use only and NOT intended for in vitro diagnostics.
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(Cat. TM33150) 100 rxns
(Cat. TM33110) 100 rxns
Kit Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Kit Size
- Detection kits for Mycoplasma DNA
- Available in TaqMan format for analysis
Mycoplasma TaqMan PCR Detection Kits
Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.
Click to expand options
- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Kit Specifications
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
Supporting Data
| Component | Cat. TM33150 (100 preps) | Cat. TM33110 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | - |
| Mycoplasma Primer & Probe Mix | 280 μL | 280 μL |
| Mycoplasma Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Mycoplasma TaqMan PCR Detection Kits
Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.
Click to expand options
- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Supporting Data
Kit Specifications
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM33150 (100 preps) | Cat. TM33110 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | - |
| Mycoplasma Primer & Probe Mix | 280 μL | 280 μL |
| Mycoplasma Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
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