• Detection kits for Mycoplasma DNA
  • Available in 2 formats for analysis:
 

ItemsCat. #Size
Mycoplasma TaqMan PCR Kit TM33100 24 rxns
Mycoplasma TaqMan Probe/Primer and Control Set TM33110 100 rxns
Mycoplasma End-Point PCR Kit EP33100 24 rxns
Mycoplasma Primer and Control Set EP33110 100 rxns

Mycoplasma Detection Kits

Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, A. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.

There are 2 kits available for the detection of Mycoplasma:

Mycoplasma TaqMan PCR Kit
  • PCR control to monitor for PCR inhibition and validate the quality
  • Master Mix for the target and PCR control detection
  • Primer and Probe mix
  • Positive control and a negative control to confirm the integrity of the kit reagents
Mycoplasma End-Point PCR Kit
  • Master Mix and primers for the specific amplification of a 327 nucleotide region of the Mycoplasma genome
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


Kit Components
Component TaqMan Probe
Cat. TM33100
(24 rxns)
End-Point
Cat. EP33100
(24 rxns)
MDx 2X PCR Master Mix - 350 µL
MDx TaqMan 2X PCR Master Mix 350 µL -
Mycoplasma Primer Mix 70 µL 70 µL
Mycoplasma Positive Control 50 µL 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Loading Dye - 100 µL
DNA Ladder - 100 µL
Product Insert 1 1
Component TaqMan
Probe/Primer
and Control Set
Cat. TM33110
(100 rxns)
Primer
and Control Set
Cat. EP33110
(100 rxns)
Mycoplasma Primer Mix 3 x 70 µL 3 x 70 µL
Mycoplasma Positive Control 3 x 50 µL 3 x 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1