|Mycoplasma TaqMan PCR Kit||TM33100||24 rxns|
|Mycoplasma TaqMan Probe/Primer and Control Set||TM33110||100 rxns|
|Mycoplasma End-Point PCR Kit||EP33100||24 rxns|
|Mycoplasma Primer and Control Set||EP33110||100 rxns|
Mycoplasma Detection Kits
Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, A. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.
There are 2 kits available for the detection of Mycoplasma:
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.
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