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Neisseria gonorrhoeae is a Gram-negative coccus of the Neisseria genus. N. gonorrhoeae is usually seen in pairs infecting human cells. It has a circular DNA genome of approximately 1Mbp encoding over 2000 genes. N. gonorrhoeae is the etiological agent of the sexually transmitted infection (STI) gonorrhoea, which globally causes an estimated 60 million new cases of gonococcal disease annually. It is second only to Chlamydia trachomatis as the most reported notifiable sexually transmitted disease. Infections with N. gonorrhoeae are primarily restricted to the mucus membranes of the endocervix, urethra, rectum, and pharynx. In females, gonorrhoea is a major cause of pelvic inflammatory disease and may lead to tubal infertility, ectopic pregnancy, and chronic pelvic pain, whereas in males it primarily causes urethritis. Importantly, these infections may often be asymptomatic, thereby contributing to further transmission and maintenance of the disease within populations.

Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard is prepared from pelleted bacteria grown on culture plates using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Neisseria gonorrhoeae.

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Figure 1. Accurate Quantity and High Quality Neisseria gonorrhoeae DNA Standard. Two micro-liters of Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard and various serial dilutions (blue curves) were used in a 20 µL Neisseria gonorrhoeae-specific PCR reaction using Norgen’s 2X PCR Mastermix (#28007), supplemented with SYBR Green I. The real-time PCR reactions were performed on a Bio-Rad iCycler iQTM Real-Time PCR System. No template controls with water were also performed (yellow curves). Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard and various serial dilutions showed consistent amplification down to as little as 40 copies per reaction.

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