NGS Library Quantification Kit (for Small RNA-Seq)
PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations
For research use only and NOT intended for in vitro diagnostics.
NGS Library Quantification Kit (for Small RNA-Seq)
PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations
Overview
- Able to quantify NGS Library (Illumina) of a wide spectrum of concentrations, including sub-nanomolar concentrations
- DNA is accurately quantified by using a standard curve constructed from the provided DNA Standards
- Specially designed DNA standards for Small RNA-Seq library; also compatible to NGS library of other molecular weights
Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) offers a PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations. The kit consists of a specially designed primer mix compatible with the Illumina system, that is used in conjunction with the provided 2X Real-Time PCR Master Mix to amplify a library of unknown concentration. The unknown library is accurately quantified by using a standard curve constructed from the provided DNA Standard (range from 20 pM to 2 fM) on a Real-Time PCR System. The kit is specially optimized to quantify Small RNA-Seq libraries with DNA standards that have similar size to a Small RNA-Seq library. However, it could also be used with other types of NGS libraries.
Details
Supporting Data
Storage Conditions
Upon receipt, store Norgen's NGS Library Quantification Kit (for Small RNA-Seq) at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
Component | Cat. 61600 (100 reactions) |
---|---|
2X Real-Time PCR Master Mix | 1 mL |
NGS Library Quantification Primer Set Mix | 600 µL |
Quantified NGS Library Standards (for Small-RNA Seq)* | 5 x 80 µL Standards |
Product Insert | 1 |
* Quantified Library Standards are provided as 20 pM, 2 pM, 200 fM, 20 fM and 2 fM
Documentation
Citations
Title | Hydrogel-Embedded Poly(Lactic-co-Glycolic Acid) Microspheres for the Delivery of hMSC-Derived Exosomes to Promote Bioactive Annulus Fibrosus Repair |
Journal | SAGE Journals. 2022. |
Authors | Tyler J., Keti Vaso, and James C. Iatridis, et al. |
Title | Alteration of miRNAs in Small Neuron‑Derived Extracellular Vesicles of Alzheimer's Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses |
Journal | Journal of Molecular Neuroscience . 2022. |
Authors | Devrim Yagmur Durur, Bora Tastan, Kemal Ugur Tufekci, Melis Olcum, Hamdiye Uzuner, Gökhan Karakülah, Gorsev Yener, Sermin Genc |