Features and Benefits

Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) offers a PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations.  The kit consists of a specially designed primer mix compatible with the Illumina system, that is used in conjunction with the provided 2X Real-Time PCR Master Mix to amplify a library of unknown concentration. The unknown library is accurately quantified by using a standard curve constructed from the provided DNA Standard (range from 20 pM to 2 fM) on a Real-Time PCR System. The kit is specially optimized to quantify Small RNA-Seq libraries with DNA standards that have similar size to a Small RNA-Seq library. However, it could also be used with other types of NGS libraries.

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Supporting Data

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Figure 1. A representative qPCR baseline graph showing the successful amplification of Quantified NGS Library Standards (Green) with a range from 20 pM to 2 fM, using Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) (Panel A). Duplicate amplification of a sample Small RNA-Seq library (at 1:10,000 dilution) was performed (Orange). The derived library concentration was 9.41 nM. Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) is of good quality as shown with the high PCR efficiency and correlation in the standard curve (Panel B) with low background signals (No Template Control – NTC as Yellow in Panel A).

Figure 2. Accurate standard curve quantification. Norgen's NGS Library Quantification Kit (for Small RNA-Seq) was compared head-to-head with two competitor's NGS Library Quantification Kits on a Bio-Rad CFX96 Real-Time PCR system. Norgen's kit showed both high correlation and PCR efficiency when compared to the competitors' kits.

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