• Able to quantify NGS Library (Illumina) of a wide spectrum of concentrations, including sub-nanomolar concentrations
  • DNA is accurately quantified by using a standard curve constructed from the provided DNA Standards
  • Specially designed DNA standards for Small RNA-Seq library; also compatible to NGS library of other molecular weights

ItemsCat. #Size
NGS Library Quantification Kit (for Small RNA-Seq) 61600 100 rxns

NGS Library Quantification Kit (for Small RNA-Seq)

Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) offers a PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations. The kit consists of a specially designed primer mix compatible with the Illumina system, that is used in conjunction with the provided 2X Real-Time PCR Master Mix to amplify a library of unknown concentration. The unknown library is accurately quantified by using a standard curve constructed from the provided DNA Standard (range from 20 pM to 2 fM) on a Real-Time PCR System. The kit is specially optimized to quantify Small RNA-Seq libraries with DNA standards that have similar size to a Small RNA-Seq library. However, it could also be used with other types of NGS libraries.

Storage Conditions
Upon receipt, store Norgen's NGS Library Quantification Kit (for Small RNA-Seq) at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.


Kit Components
Component Cat. 61600 (100 reactions)
2X Real-Time PCR Master Mix 1 mL
NGS Library Quantification Primer Set Mix 600 µL
Quantified NGS Library Standards (for Small-RNA Seq)* 5 x 80 µL Standards
Product Insert 1

* Quantified Library Standards are provided as 20 pM, 2 pM, 200 fM, 20 fM and 2 fM