NGS Normalization 96-Well Kit

Cat. 61900
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NGS Normalization 96-Well Kit

Cat. 61900

For high-throughput NGS Library PCR amplicon clean-up and normalization of NGS library PCR product concentration

  • Removes primer dimers
  • Simultaneously clean-up and normalize PCR products 
  • Fast (less than 20 minutes), high-throughput and easy processing using centrifugation
  • Sufficient elution volume (100 µL) for repeat or future assays 
  • Non-magnetic bead purification 

For research use only and NOT intended for in vitro diagnostics.

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NGS Normalization 96-Well Kit
(Cat. 61900)
2 x 96-well plates

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 61900

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Title The persistence and stabilization of auxiliary genes in the human skin virome
Journal Virology Journal. 2022.
Authors Ema H. Graham, Wesley A. Tom, Alison C. Neujahr, Michael S. Adamowicz, Jennifer L. Clarke, Joshua R. Herr & Samodha C. Fernando

NGS Normalization 96-Well Kit

Norgen's NGS Normalization 96-Well Kit allows for high-throughput NGS library PCR amplicon clean-up and normalization of NGS library PCR product concentration. The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides during the library prep PCR step. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized library PCR amplicon can then be used in NGS workflows and other sequencing applications.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 61900

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Title The persistence and stabilization of auxiliary genes in the human skin virome
Journal Virology Journal. 2022.
Authors Ema H. Graham, Wesley A. Tom, Alison C. Neujahr, Michael S. Adamowicz, Jennifer L. Clarke, Joshua R. Herr & Samodha C. Fernando

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