Name: Olive Oil DNA Isolation Kit
Brand: Norgen Biotek Corp.
SKU: 61700
Price: 179.000000 USD
Availability: InStock

Overview

Fast and easy processing using a rapid spin-column format
Isolate total DNA without compromising total yield
No phenol or chloroform extractions
Isolate high quality total DNA from a variety of oil samples in order to support genetic traceability by allowing for the verification of the authenticity of the oil, thereby discouraging adulteration

Norgen’s Olive Oil DNA Isolation Kit is designed for the rapid preparation of genomic DNA from olive oil in order to support genetic traceability by allowing for the verification of the authenticity of the oil, thereby discouraging adulteration. Norgen’s Olive Oil DNA Isolation Kit can also be used to purify DNA from other processed oils such as canola oil, sunflower oil or vegetable oil to allow for downstream analysis and testing for oil adulteration. Genomic DNA is efficiently extracted from 0.5 mL of oil by a combination of heat treatment, homogenization and a unique lysis buffer. PCR inhibitors are effectively removed during the washing steps and the purified genomic DNA is ready to use for downstream applications such as PCR and microarray analysis. Preparation time for ten samples is less then 30 minutes, and each kit contains sufficient materials for 50 preparations.

Details

Supporting Data

Figure 1. Process a Variety of Different Food Oils. A variety of different food oils purchased from stores can be processed for DNA isolation using Norgen's Olive Oil DNA Isolation Kit. The high quality of the purified DNA is shown in the Random Amplified Polymorphic DNA (RAPD) analysis (See Figure 2).

Figure 2. Random Amplified Polymorphic DNA (RAPD) Analysis of 10 Food Oil Products. Inputs of 0.5 mL of oil were processed using Norgen’s Olive Oil DNA Isolation Kit, and 5 µL of the 40 µL eluted DNA was used in 20 µL of RAPD reaction. The RAPD pattern indicates successful RAPD amplification with high DNA quality. Ma: Norgen’s UltraRanger 1 kb DNA Ladder (Cat. 12100), Mb: HighRanger 1 kb DNA Ladder (Cat. 11900).

Kit Specifications
Maximum Column Binding Capacity	10 µg
Maximum Column Loading Volumes	650 µL
Time to Complete 10 Purifications	25 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 61700 (50 preps)
Lysis Buffer L	60 mL
Binding Buffer B	40 mL
Wash Solution A	38 mL
Elution Buffer B	8 mL
Homogenization Tubes	50
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Documentation

PROTOCOLS

(61700) Olive Oil DNA Isolation Kit - Protocol (50 preps)

SAFETY DATA SHEETS

61700 - Olive Oil DNA Isolation Kit - SDS

FAQs

Spin Column

I noticed the column was clogged. What causes this?

Clogged columns may occur due to improper oil removal. Too much oil was carried over to the lysate. Repeat Step 1d and 1e to minimize the oil carry over. Clogging can be alleviated by increasing the g-force and/or centrifuging for a longer period of time until the lysate passes through the column.

Why is the purified DNA not performing well in downstream applications?

If the DNA does not perform well in downstream applications, it may be due to one or more of the following:

Low DNA yield.
Depending on the oil extraction process, DNA can be damaged or lost. Increase the input volume up to 1 mL, as well as the other buffers and solutions proportionally.
Ethanol carryover.
Ensure that the dry spin under the Column Wash procedure is performed in order to remove traces of ethanol prior to elution. Ethanol is known to interfere with many downstream applications.
The column was not washed twice with the provided Wash Solution A.
Traces of salt from the binding step may remain in the sample if the column is not washed twice with Wash Solution A. Salt may interfere with downstream applications, and thus must be washed from the column.
PCR reaction conditions need to be optimized.
Take steps to optimize the PCR conditions being used, including varying the amount of template (20 ng to 50 ng for 20 µL of PCR reaction), changing the source of Taq polymerase, adding BSA (final concentration is 0.1 µg/µL), looking into the primer design, and adjusting the annealing conditions.

What is average yield from Olive Oil DNA isolation kit?

The yield varies a lot with the variety and processing status of the olive oil.

Citations

Title	Application of high resolution melting combined with DNA-based markers for quantitative analysis of olive oil authenticity and adulteration
Journal	Food Chemistry. 2020
Authors	Elsa Chedid, Myrto Rizou, Panagiotis Kalaitzis

Olive Oil DNA Isolation Kit