Features and Benefits

Norgen's PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) provides a rapid, simple and efficient procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on magnetic beads without the use of phenol, chloroform or alcohol precipitation.  The kit provides a high quality product with up to 90% recovery.

Norgen's PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

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Figure 1. Efficient Clean-Up and Recovery. The performance of Norgen's PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) was compared to Norgen's column-based PCR Purification Kit and Sequencing Reaction Clean-Up Kit by purifying and cleaning 20 µL of 16S NGS library reaction and an end-point PCR. For evaluation, 10 µL of each 50 µL elution were run on a 1X TAE 1.4% agarose gel. As it can be seen, Norgen's PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) was able to successfully recover the target bands without any primer dimer contamination. Marker = Norgen's FastRunner DNA Ladder.

Figure 2. Efficient Clean-Up and Recovery. The performance of Norgen's PCR and Sequencing Reaction Clean-Up 96-Well Kit (Magnetic Bead System) was tested by purifying and cleaning 20 µL of 16S NGS library reaction and an end-point PCR. For evaluation, 10 µL of each 50 µL elution were run on a 1X TAE 1.4% agarose gel. As it can be seen, Norgen's PCR and Sequencing Reaction Clean-Up 96-Well Kit (Magnetic Bead System) was able to successfully recover the target bands without any primer dimer contamination. Marker = Norgen's FastRunner DNA Ladder (Cat. 12800).

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