Phomopsis viticola TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Phomopsis viticola TaqMan PCR Detection Kits Price USD$699.00 SKU TM34950 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for the Phomopsis viticola Available in TaqMan format for analysis Phomopsis cane and leaf spot, caused by Phomopsis viticola, occurs in most grape-growing regions and is an important disease of grapes worldwide. The fungus attacks all green parts of the vine, including canes, leaves, flowers, rachises, and berries. However, most economic damage is caused by rachis and berry infection. Infected leaves have small, yellowish spots with dark brown centers and may be puckered. With successive cool, wet springs, the inoculum will build up and infections will become more severe over time. Since symptoms appear 21 to 30 days after infection, early detection of the pathogen in the vineyard is therefore crucial for its control. TaqMan PCR Kit Click to expand options Phomopsis viticola TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Phomopsis viticola TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM34950 (100 preps) Cat. TM34910 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - Phomopsis viticola Primer & Probe Mix 280 μL 280 μL Phomopsis viticola Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM34950) Phomopsis viticola TaqMan PCR Kit - Protocol (100 rxns) Related Products Phomopsis viticola TaqMan Probe/Primer and Control Set Fungi/Yeast Genomic DNA Isolation Kit
Phomopsis viticola TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Phomopsis viticola TaqMan PCR Detection Kits Price USD$699.00 SKU TM34950 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity
