Plant/Fungi DNA Isolation Kit (Cat. 26200, 26250, 26900)
![Plant/Fungi DNA Isolation Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/26200-Reflection-web.jpg?itok=olPGEWr_" "Plant/Fungi DNA Isolation Kit")
- Rapid and simple procedure
- Excellent quality and yield of DNA
- Process a broad spectrum of plant species and filamentous fungi
- Isolate total DNA including pathogen DNA without phenol
- Available in spin column format and 96-well format for high throughput applications
For rapid isolation of total DNA from plants and fungi
Want a discount?
Create your account to receive 15% off on your first purchase!
Plant/Fungi DNA Isolation Kit (Cat. 26200)
50 preps
Plant/Fungi DNA Isolation Kit (Cat. 26250)
250 preps
Plant/Fungi DNA Isolation 96-Well Kit (Cat. 26900)
2 x 96-well plates
- Rapid and simple procedure
- Excellent quality and yield of DNA
- Process a broad spectrum of plant species and filamentous fungi
- Isolate total DNA including pathogen DNA without phenol
- Available in spin column format and 96-well format for high throughput applications
Click for expanded view
Figure 1. Isolate DNA from a Wide Range of Plants
Figure 2. High Quality DNA
Plant/Fungi DNA Isolation Kit
This kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The protocol can be completed in 45 minutes. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
This kit is also available in a 96-well format for high throughput applications.
|
Kit Specifications - Spin Column
|
|
| Column Binding Capacity |
50 μg
|
| Maximum Column Loading Volume |
650 μL
|
|
Maximum Amount of Starting Material:
Plant Tissues
Fungi (wet weight)
|
100 mg
100 mg |
| Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) |
18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
|
Kit Specifications - 96-well
|
|
| Binding Capacity Per Well |
50 μg
|
| Maximum Loading Volume Per Well |
500 μL
|
| Size of DNA Purified |
All sizes |
| Maximum Amount of Starting Material: Fungi Plant Tissues |
50 mg (wet weight) 50 mg |
| Average Yields* Grape leaf Apple leaf Strawberry leaf Plum leaf Peach leaf Peach petiole |
7 µg 3 µg 6 µg 4 µg 5 µg 4 µg |
| Time to Complete 96 Purifications |
50 minutes
|
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. These reagents should remain stable for at least 2 years in their unopened containers.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont'd) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
Figure 1. Isolate DNA from a Wide Range of Plants
Figure 2. High Quality DNA
Product Information Sheets:
Application Notes:
Posters:
Safety Data Sheets:
| Title | Cultivar-specific gene modulation in Vitis vinifera: analysis of the promoters regulating the expression of WOX transcription factors |
| Journal | Scientific Reports. 2017 |
| Authors | Boccacci P, Mela A, Pavez Mina C, Chitarra W, Perrone I, Gribaudo I, Gambino G. |
| Title | Lotus japonicus NF-YA1 plays an essential role during nodule differentiation and targets members of the SHI/STY gene family |
| Journal | Molecular plant-microbe interactions: MPMI. 2016. |
| Authors | Hossain MS, Shrestha A, Zhong S, Miri M, Austin RS, Sato S, Ross L, Huebert T, Tromas A, Torres-Jerez I, Tang Y, Udvardi M, Murray JD, Szczyglowski K. |
| Title | Numerical and RAPD Analysis of Eight Cowpea Genotypes from Nigeria |
| Journal | Nigerian Journal of Biotechnology. 2016. |
| Authors | E.O. Idehen, S.A. Oyelakin, A.O. Onikola, V.O. Adedapo |
| Title | Chemical Control of Black Scorch Disease on Date Palm Caused by the Fungal Pathogen, Thielaviopsis punctulata in United Arab Emirates |
| Journal | Plant Disease. 2016. |
| Authors | Esam Eldin Saeed, Arjun Sham, Khaled Abbas El-Tarabily, Firas Abu Elsamen, Rabah Iratni, Synan F. AbuQamar |
| Title | Identification Of Cuscuta Campestrisyuncker In Uae: Study Of Bar Code Loci-Rbcl, Matk And Trnh-Psbain The Uae And Egyptian Cultivars And In The Respective Host Plants Basil And Jute |
| Journal | International Journal of Research in Science. 2016. |
| Authors | Alsaadi, A., Saif, M., Yasser, M., Gaur, S., El-wakil, H., Maloukh, L., & Lakshmi, T. V. R |
| Title | Potential biosurfactant producing endophytic and epiphytic fungi, isolated from macrophytes in the Negro River in Manaus, Amazonas, Brazil |
| Journal | African Journal of Biotechnology. 2016. |
| Authors | Lima, J. M. S., Pereira, J. E. O., Batista, I. H., Neto, P. D. Q. C., dos Santos, J. C., de Ara&ujo, S. P., ... & de Azevedo, J. L. U |
| Title | Fate of transgenic deoxyribonucleic acid fragments in digesta and tissues of rabbits fed genetically modified soybean meal |
| Journal | Journal of Animal Science. 2015. |
| Authors | P. Morera, L. Basiricò, B. Ronchi and U. Bernabucci |
| Title | MiRNA-Mediated Regulation of the SWI/SNF Chromatin Remodeling Complex Controls Pluripotency and Endodermal Differentiation in Human ESCs |
| Journal | Stem Cells. 2015. |
| Authors | Wade SL, Langer LF, Ward JM, Archer TK |
| Title | Mangrove Rhizosphere Soils: A Unique Natural Source of Pravastatin-Producing Penicillium Microfungi |
| Journal | International Journal of Extensive Research. 2015. |
| Authors | Seydametova Emine, Norazwina Zainol, Jailani Salihon and Peter Convey |
| Title | A stable JAZ protein from peach mediates the transition from outcrossing to self-pollination |
| Journal | BMC Biology. 2015. |
| Authors | Sherif Sherif, Islam El-Sharkawy, Jaideep Mathur, Pratibha Ravindran, Prakash Kumar, Gopinadhan Paliyath and Subramanian Jayasankar |
| Title | Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues. |
| Journal | Applications in Plant Sciences. 2014. |
| Authors | Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey. |
| Title | Establishment of invasive and non-invasive reporter systems to investigate American elm–Ophiostoma novo-ulmi interactions. |
| Journal | Fungal Genetics and Biology. 2014. |
| Authors | S. Sherif, A.M.P. Jones, M.R. Shukla, P.K. Saxena. |
| Title | Aplification of TRP1 for Specific Detection of Phytophthora Ramorum. |
| Journal | United States Patent Application 022057. 2014. |
| Authors | Bohannon, Robert C., Russell Jr., Paul F. |
| Title | Development of a Real-Time Microchip PCR System for Portable Plant Disease Diagnosis. |
| Journal | PLoS One. 2013. |
| Authors | Koo C, Malapi-Wight M, Kim H, Cifci O, Vaughn-Diaz V, Ma B, Kim S, Abdel-Raziq H, Ong K, Jo Y, Gross D, Shim W, Han A |
| Title | Optimization of decolorization of palm oil mill effluent (POME) by growing cultures of Aspergillus fumigatus using response surface methodology. |
| Journal | Environmental Science and Pollution Research. 2012. |
| Authors | Neoh C, Yahya A, Adnan R, Majid Z and Ibrahim Z. |
| Title | Three sympatrically occurring species of Metarhizium show plant rhizosphere specificity. |
| Journal | Journal of Microbiology. 2011. |
| Authors | Wyrebek M, Huber C, Sasan RK, Bidochka MJ. |
| Title | Molecular cloning and expression analysis of a delta 6-fatty acid desaturase gene from Rhizopus stolonifer strain YF6 which can accumulate high levels of gamma-linolenic acid. |
| Journal | Journal of Microbiology. 2011. |
| Authors | Wan X, Zhang Y, Wang P, Jiang M. |
| Title | Flexible metabolism in Metarhizium anisopliae and Beauveria bassiana: utility of the glyoxylate cycle during insect pathogenesis |
| Journal | Microbiology. 2011. |
| Authors | Padilla-Guerrero IE, Barelli L, Gonzˆlez-Hernˆndez GA, Torres-Guzmˆn JC, Bidochka M. |
Want a discount?
Create your account to receive 15% off on your first purchase!
Full Kits
Plant/Fungi DNA Isolation Kit (Cat. 26200)
50 preps
Plant/Fungi DNA Isolation Kit (Cat. 26250)
250 preps
Plant/Fungi DNA Isolation 96-Well Kit (Cat. 26900)
2 x 96-well plates
- Rapid and simple procedure
- Excellent quality and yield of DNA
- Process a broad spectrum of plant species and filamentous fungi
- Isolate total DNA including pathogen DNA without phenol
- Available in spin column format and 96-well format for high throughput applications
Click for expanded view
Figure 1. Isolate DNA from a Wide Range of Plants
Figure 2. High Quality DNA
Plant/Fungi DNA Isolation Kit
This kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The protocol can be completed in 45 minutes. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
This kit is also available in a 96-well format for high throughput applications.
|
Kit Specifications - Spin Column
|
|
| Column Binding Capacity |
50 μg
|
| Maximum Column Loading Volume |
650 μL
|
|
Maximum Amount of Starting Material:
Plant Tissues
Fungi (wet weight)
|
100 mg
100 mg |
| Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) |
18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
|
Kit Specifications - 96-well
|
|
| Binding Capacity Per Well |
50 μg
|
| Maximum Loading Volume Per Well |
500 μL
|
| Size of DNA Purified |
All sizes |
| Maximum Amount of Starting Material: Fungi Plant Tissues |
50 mg (wet weight) 50 mg |
| Average Yields* Grape leaf Apple leaf Strawberry leaf Plum leaf Peach leaf Peach petiole |
7 µg 3 µg 6 µg 4 µg 5 µg 4 µg |
| Time to Complete 96 Purifications |
50 minutes
|
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. These reagents should remain stable for at least 2 years in their unopened containers.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont'd) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
Figure 1. Isolate DNA from a Wide Range of Plants
Figure 2. High Quality DNA
Product Information Sheets:
Application Notes:
Posters:
Safety Data Sheets:
| Title | Cultivar-specific gene modulation in Vitis vinifera: analysis of the promoters regulating the expression of WOX transcription factors |
| Journal | Scientific Reports. 2017 |
| Authors | Boccacci P, Mela A, Pavez Mina C, Chitarra W, Perrone I, Gribaudo I, Gambino G. |
| Title | Lotus japonicus NF-YA1 plays an essential role during nodule differentiation and targets members of the SHI/STY gene family |
| Journal | Molecular plant-microbe interactions: MPMI. 2016. |
| Authors | Hossain MS, Shrestha A, Zhong S, Miri M, Austin RS, Sato S, Ross L, Huebert T, Tromas A, Torres-Jerez I, Tang Y, Udvardi M, Murray JD, Szczyglowski K. |
| Title | Numerical and RAPD Analysis of Eight Cowpea Genotypes from Nigeria |
| Journal | Nigerian Journal of Biotechnology. 2016. |
| Authors | E.O. Idehen, S.A. Oyelakin, A.O. Onikola, V.O. Adedapo |
| Title | Chemical Control of Black Scorch Disease on Date Palm Caused by the Fungal Pathogen, Thielaviopsis punctulata in United Arab Emirates |
| Journal | Plant Disease. 2016. |
| Authors | Esam Eldin Saeed, Arjun Sham, Khaled Abbas El-Tarabily, Firas Abu Elsamen, Rabah Iratni, Synan F. AbuQamar |
| Title | Identification Of Cuscuta Campestrisyuncker In Uae: Study Of Bar Code Loci-Rbcl, Matk And Trnh-Psbain The Uae And Egyptian Cultivars And In The Respective Host Plants Basil And Jute |
| Journal | International Journal of Research in Science. 2016. |
| Authors | Alsaadi, A., Saif, M., Yasser, M., Gaur, S., El-wakil, H., Maloukh, L., & Lakshmi, T. V. R |
| Title | Potential biosurfactant producing endophytic and epiphytic fungi, isolated from macrophytes in the Negro River in Manaus, Amazonas, Brazil |
| Journal | African Journal of Biotechnology. 2016. |
| Authors | Lima, J. M. S., Pereira, J. E. O., Batista, I. H., Neto, P. D. Q. C., dos Santos, J. C., de Ara&ujo, S. P., ... & de Azevedo, J. L. U |
| Title | Fate of transgenic deoxyribonucleic acid fragments in digesta and tissues of rabbits fed genetically modified soybean meal |
| Journal | Journal of Animal Science. 2015. |
| Authors | P. Morera, L. Basiricò, B. Ronchi and U. Bernabucci |
| Title | MiRNA-Mediated Regulation of the SWI/SNF Chromatin Remodeling Complex Controls Pluripotency and Endodermal Differentiation in Human ESCs |
| Journal | Stem Cells. 2015. |
| Authors | Wade SL, Langer LF, Ward JM, Archer TK |
| Title | Mangrove Rhizosphere Soils: A Unique Natural Source of Pravastatin-Producing Penicillium Microfungi |
| Journal | International Journal of Extensive Research. 2015. |
| Authors | Seydametova Emine, Norazwina Zainol, Jailani Salihon and Peter Convey |
| Title | A stable JAZ protein from peach mediates the transition from outcrossing to self-pollination |
| Journal | BMC Biology. 2015. |
| Authors | Sherif Sherif, Islam El-Sharkawy, Jaideep Mathur, Pratibha Ravindran, Prakash Kumar, Gopinadhan Paliyath and Subramanian Jayasankar |
| Title | Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues. |
| Journal | Applications in Plant Sciences. 2014. |
| Authors | Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey. |
| Title | Establishment of invasive and non-invasive reporter systems to investigate American elm–Ophiostoma novo-ulmi interactions. |
| Journal | Fungal Genetics and Biology. 2014. |
| Authors | S. Sherif, A.M.P. Jones, M.R. Shukla, P.K. Saxena. |
| Title | Aplification of TRP1 for Specific Detection of Phytophthora Ramorum. |
| Journal | United States Patent Application 022057. 2014. |
| Authors | Bohannon, Robert C., Russell Jr., Paul F. |
| Title | Development of a Real-Time Microchip PCR System for Portable Plant Disease Diagnosis. |
| Journal | PLoS One. 2013. |
| Authors | Koo C, Malapi-Wight M, Kim H, Cifci O, Vaughn-Diaz V, Ma B, Kim S, Abdel-Raziq H, Ong K, Jo Y, Gross D, Shim W, Han A |
| Title | Optimization of decolorization of palm oil mill effluent (POME) by growing cultures of Aspergillus fumigatus using response surface methodology. |
| Journal | Environmental Science and Pollution Research. 2012. |
| Authors | Neoh C, Yahya A, Adnan R, Majid Z and Ibrahim Z. |
| Title | Three sympatrically occurring species of Metarhizium show plant rhizosphere specificity. |
| Journal | Journal of Microbiology. 2011. |
| Authors | Wyrebek M, Huber C, Sasan RK, Bidochka MJ. |
| Title | Molecular cloning and expression analysis of a delta 6-fatty acid desaturase gene from Rhizopus stolonifer strain YF6 which can accumulate high levels of gamma-linolenic acid. |
| Journal | Journal of Microbiology. 2011. |
| Authors | Wan X, Zhang Y, Wang P, Jiang M. |
| Title | Flexible metabolism in Metarhizium anisopliae and Beauveria bassiana: utility of the glyoxylate cycle during insect pathogenesis |
| Journal | Microbiology. 2011. |
| Authors | Padilla-Guerrero IE, Barelli L, Gonzˆlez-Hernˆndez GA, Torres-Guzmˆn JC, Bidochka M. |
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