For rapid isolation of total DNA from plants and fungi

  • Rapid and simple procedure
  • Excellent quality and yield of DNA
  • Process a broad spectrum of plant species and filamentous fungi
  • Isolate total DNA including pathogen DNA without phenol
  • Available in spin column format and 96-well format for high throughput applications

ItemsCat. #Size
Plant/Fungi DNA Isolation Kit 26200 50 preps
Plant/Fungi DNA Isolation 96-Well Kit 26900 192 Preps

Plant/Fungi DNA Isolation Kit

This kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The protocol can be completed in 45 minutes. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.

This kit is also available in a 96-well format for high throughput applications.

Kit Specifications - Spin Column
Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Maximum Amount of Starting Material:
Plant Tissues
Fungi (wet weight)
100 mg
100 mg
Average Yields*
50 mg Tomato Leaves
50 mg Grape Leaves
50 mg Peach Leaves
50 mg Plum Leaves
50 mg Pine Needles
Botrytis cinerea (50 mg wet weight)
Fusarium sp. (50 mg wet weight)
Aspergillus niger (50 mg wet weight)
18 µg
10 µg
10 µg
10 µg
5 µg
1.5 µg
2 µg
4 µg
Time to Complete 10 Purifications 45 minutes

* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.

Kit Specifications - 96-well
Binding Capacity Per Well
50 μg
Maximum Loading Volume Per Well
500 μL
Size of DNA Purified

All sizes

Maximum Amount of Starting Material:
Fungi
Plant Tissues
50 mg (wet weight)
50 mg
Average Yields*
Grape leaf
Apple leaf
Strawberry leaf
Plum leaf
Peach leaf
Peach petiole
7 µg
3 µg
6 µg
4 µg
5 µg
4 µg
Time to Complete 96 Purifications
50 minutes

* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. These reagents should remain stable for at least 2 years in their unopened containers.

Select Plants and Fungi Tested with the Plant/Fungi DNA Purification Kits

Plants Fungi
Tomato Aspergillus niger
Grape Mucor racemosus
Peach Cladosporium cladosporioides
Plum Fusarium oxysporum
Pine Needles Penicillium sp.
Raspberry Botrytis cinerea (Botryotinia fuckeliana)
Strawberry Pichia sp.
Legumes Rhizopus oryzae
Prosopis cineraria (Ghaf) Alternaria tenuissima
Sorghum grass Fusarium sp.
Tobacco
Arabidopsis
Other
Lichen


Kit Components
Component Cat. 26200 (50 preps)
Lysis Buffer L 30 mL
Binding Buffer B 7 mL
Solution WN 18 mL
Elution Buffer B 15 mL
RNAse A 1 vial
Filter Columns 50
Spin Columns 50
Collection Tubes 100
Elution Tubes (1.7 mL) 50
Product Insert 1
Component Cat. 26900 (2 x 96-well)
Lysis Buffer L 2 x 60 mL
Binding Buffer I 25 mL
Solution WN 55 mL
Wash Solution A 2 x 38 mL
Elution Buffer B 30 mL
RNAse A 1 vial
96-Well Plate 2
Adhesive Tape 4
96-Well Collection Plate 2
96-Well Elution Plate 2
Product Insert 1

Title Mangrove Rhizosphere Soils: A Unique Natural Source of Pravastatin-Producing Penicillium Microfungi
Journal International Journal of Extensive Research. 2015.
Authors Seydametova Emine, Norazwina Zainol, Jailani Salihon and Peter Convey
Title A stable JAZ protein from peach mediates the transition from outcrossing to self-pollination
Journal BMC Biology. 2015.
Authors Sherif Sherif, Islam El-Sharkawy, Jaideep Mathur, Pratibha Ravindran, Prakash Kumar, Gopinadhan Paliyath and Subramanian Jayasankar
Title Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues.
Journal Applications in Plant Sciences. 2014.
Authors Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey.
Title Establishment of invasive and non-invasive reporter systems to investigate American elm–Ophiostoma novo-ulmi interactions.
Journal Fungal Genetics and Biology. 2014.
Authors S. Sherif, A.M.P. Jones, M.R. Shukla, P.K. Saxena.
Title Aplification of TRP1 for Specific Detection of Phytophthora Ramorum.
Journal United States Patent Application 022057. 2014.
Authors Bohannon, Robert C., Russell Jr., Paul F.
Title Development of a Real-Time Microchip PCR System for Portable Plant Disease Diagnosis.
Journal PLoS One. 2013.
Authors Koo C, Malapi-Wight M, Kim H, Cifci O, Vaughn-Diaz V, Ma B, Kim S, Abdel-Raziq H, Ong K, Jo Y, Gross D, Shim W, Han A
Title Optimization of decolorization of palm oil mill effluent (POME) by growing cultures of Aspergillus fumigatus using response surface methodology.
Journal Environmental Science and Pollution Research. 2012.
Authors Neoh C, Yahya A, Adnan R, Majid Z and Ibrahim Z.
Title Three sympatrically occurring species of Metarhizium show plant rhizosphere specificity.
Journal Journal of Microbiology. 2011.
Authors Wyrebek M, Huber C, Sasan RK, Bidochka MJ.
Title Flexible metabolism in Metarhizium anisopliae and Beauveria bassiana: utility of the glyoxylate cycle during insect pathogenesis
Journal Microbiology. 2011.
Authors Padilla-Guerrero IE, Barelli L, Gonzˆlez-Hernˆndez GA, Torres-Guzmˆn JC, Bidochka M.