Plant/Fungi DNA Isolation Kits (Cat. 26200, 26250, 26900, 58200, 62400)
Plant/Fungi DNA Isolation Kits
![Plant/Fungi DNA Isolation Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/26200-Reflection-web.jpg?itok=dpvnBUpl" "Plant/Fungi DNA Isolation Kit")
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*Inquire by phone or email for more information.
What Scientists are Saying About this Product
In my PhD project, I had difficulty in isolation RNA and DNA, especially RNA, from plum. I am glad that all the difficulty was over since I used Plant RNA and Plant/Fungi DNA isolation kits from NORGEN. The yield and purity are all excellent. I would like to thank NORGEN for providing such good products.
- Agriculture and Agrifood Canada
Plant/Fungi DNA Isolation Kits
For rapid isolation of total DNA from plants and fungi
- Rapid and simple procedure
- Excellent quality and yield of DNA
- Process a broad spectrum of plant species and filamentous fungi
- Isolate total DNA including pathogen DNA without phenol
- Available in spin column format and 96-well format for high throughput applications
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 26200) 50 preps
(Cat. 26250) 250 preps
(Cat. 26900) 2 x 96-well plates
(Cat. 58200) 50 preps
(Cat. 62400) 2 x 96-well plates
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
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Kit Specifications
|
Kit Specifications - Spin Column
|
|
| Column Binding Capacity |
50 μg
|
| Maximum Column Loading Volume |
650 μL
|
|
Maximum Amount of Starting Material:
Plant Tissues
Fungi (wet weight)
|
100 mg
100 mg |
| Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) |
18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont'd) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
|
Kit Specifications - Spin Column
|
|
| Column Binding Capacity |
50 μg
|
| Maximum Column Loading Volume |
650 μL
|
|
Maximum Amount of Starting Material:
Plant Tissues
Fungi (wet weight)
|
100 mg
100 mg |
| Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) |
18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont'd) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
|
Kit Specifications - 96-well
|
|
| Binding Capacity Per Well |
50 μg
|
| Maximum Loading Volume Per Well |
500 μL
|
| Size of DNA Purified |
All sizes |
| Maximum Amount of Starting Material: Fungi Plant Tissues |
50 mg (wet weight) 50 mg |
| Average Yields* Grape leaf Apple leaf Strawberry leaf Plum leaf Peach leaf Peach petiole |
7 µg 3 µg 6 µg 4 µg 5 µg 4 µg |
| Time to Complete 96 Purifications |
50 minutes
|
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNase A which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont'd) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
|
Kit Specifications
|
|
|
Maximum Plant Input
|
50 mg
|
|
Type of Plants Processed
|
All types of plant species and tissue
|
|
Average Yield from 50 mg of Plant*
|
3-10 μg
|
| Average Purity (OD260/280) |
1.8 - 2.0
|
| Time to Complete 12 Purifications |
50 minutes
|
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
Store RNAse A at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
|
Kit Specifications
|
|
|
Maximum Plant Input
|
50 mg
|
|
Type of Plants Processed
|
All types of plant species and tissue
|
|
Average Yield from 50 mg of Plant*
|
3-10 μg
|
| Average Purity (OD260/280) |
1.8 - 2.0
|
| Time to Complete 96 Purifications |
Less than 60 minutes
|
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
Store RNase A at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Supporting Data
| Component | Cat. 26200 (50 preps) | Cat. 26250 (250 preps) | Cat. 26900 (192 preps) | Cat. 58200 (50 preps) | Cat. 62400 (192 preps) |
|---|---|---|---|---|---|
| Lysis Buffer L | 30 mL | 1 x 30 mL 2 x 60 mL |
2 x 60 mL | 60 mL | 2 x 60 mL |
| Binding Buffer I | 7 mL | 1 x 7 mL 1 x 25 mL |
25 mL | 7 mL | 25 mL |
| Solution WN | 18 mL | 1 x 18 mL 1 x 55 mL |
55 mL | 18 mL | 55 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | - | - |
| Elution Buffer B | 15 mL | 30 mL | 30 mL | 8 mL | 30 mL |
| RNAse A | 1 vial (80 μL) | 5 vials (5 x 80 μL) | 1 vial | 1 vial | 1 vial |
| Magnetic Bead Suspension | - | - | - | 4 x 1.1 mL | 2 x 8.5 mL |
| Filter Columns | 50 | 250 | - | - | - |
| Spin Columns | 50 | 250 | - | - | - |
| Collection Tubes | 100 | 500 | - | - | - |
| 96-Well Plate | - | - | 2 | - | 2 |
| 96-Well Collection Plate | - | - | 2 | - | - |
| Adhesive Tape | - | - | 4 | - | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | - | 50 | - |
| 96-Well Elution Plate | - | - | 2 | - | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 |
Spin Column, HT (26200, 26250, 26900)
| Title | Leaf Bacteriome in Sugar Beet Shows Differential Response against Beet curly top virus during Resistant and Susceptible Interactions |
| Journal | Int. J. Mol. Sci . 2022 |
| Authors | Rajtilak Majumdar, Carl A. Strausbaugh, Eric D. Vincill, Imad Eujayl and Paul J. Galewski |
| Title | Chitosan Sensitivity of Fungi Isolated from Mango (Mangifera indica L.) with Anthracnose |
| Journal | Molecules . 2022 |
| Authors | Griselda Valenzuela-Ortiz, Soila Maribel Gaxiola-Camacho, Cesar San-Martín-Hernández, Miguel Ángel Martínez-Téllez, Emmanuel Aispuro-Hernández, Jaime Lizardi-Mendoza and Eber Addí Quintana-Obregón |
| Title | L-Asparaginase from Penicillium sizovae Produced by a Recombinant Komagataella phaffii Strain |
| Journal | Pharmaceuticals . 2022 |
| Authors | Marcela Freitas, Paula Souza, Mauricio Homem-de-Mello, Yris M. Fonseca-Bazzo, Damaris Silveira, Edivaldo X. Ferreira Filho, Adalberto Pessoa Junior, Dipak Sarker, David Timson, João Inácio and Pérola O. Magalhães |
| Title | Agaricus macrochlamys, a New Species from the (Sub)tropical Cloud Forests of North America and the Caribbean, and Agaricus fiardii, a New Synonym of Agaricus subrufescens |
| Journal | J. Fungi . 2022 |
| Authors | Rosario Medel-Ortiz, Roberto Garibay-Orijel, Andrés Argüelles-Moyao, Gerardo Mata, Richard W. Kerrigan, Alan E. Bessette, József Geml, Claudio Angelini, Luis A. Parra and Jie Chen |
| Title | Marker assisted selection (MAS) for downy mildew resistance in grapevines using Rpv3.1 associated markers |
| Journal | Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2022 |
| Authors | Murat AKKURT, Irem ŞENSES, Burak AKTÜRK, Ilhami TOZLU, Ilhami TOZLU and Halil Ibrahim UZUN |
| Title | New Antifungal Compound, 6-Pentyl-α-Pyrone, against the Maize Late Wilt Pathogen, Magnaporthiopsis maydis |
| Journal | Agronomy. 2022 |
| Authors | Ofir Degani, and Asaf Gordani |
| Title | Genomic analyses point to a low evolutionary potential of prospective source populations for assisted migration in a forest herb |
| Journal | Evolutionary Applications. 2022 |
| Authors | Frederik Van Daele,Olivier Honnay,Hanne De Kort |
| Title | Common facultative endosymbionts do not influence sensitivity of cereal aphids to pyrethroids |
| Journal | RES JOUNRALS. 2022 |
| Authors | Daniel J. Leybourne, Petra Melloh, Emily A. Martin |
| Title | Emergence and Genomic Characterization of Multidrug Resistant Candida auris in Nigeria, West Africa |
| Journal | J. Fungi. 2022 |
| Authors | Rita Oladele, Jessica N. Uwanibe, Idowu B. Olawoye, Abdul-Wahab O. Ettu, Jacques F. Meis and Christian T. Happi |
| Title | Alternaria species causing pomegranate and citrus fruit rots in Albania |
| Journal | Journal of Plant Diseases and Protection. 2022 |
| Authors | Magdalena Cara, Mirela Toska, Dajana Frasheri, Riccardo Baroncelli & Simona Marianna Sanzani |
| Title | Evaluation of Three Forms of Rhizoctonia solani Mediated Pathogenicity to Sugar Beet Cultivars in Greenhouse Studies |
| Journal | bioRxiv. 2022 |
| Authors | Md Ehsanul Haque, Most Shanaj Parvin |
| Title | Phylogeographic patterns in cryptic Bostrychia tenella species (Rhodomelaceae, Rhodophyta) across the Thai-Malay Peninsula |
| Journal | Algae. 2022 |
| Authors | Jakaphan Bulan, Sinchai Maneekat, Giuseppe C. Zuccarello, Narongrit Muangmai |
| Title | Metabarcoding assessment of fungal diversity in brown algae and sponges of Mauritius |
| Journal | Frontiers in Microbiology. 2022 |
| Authors | Jessica Mélanie Wong Chin, Daneshwar Puchooa, Theeshan Bahorun, Vidushi S. Neergheen, Aadil Ahmad Aullybux, Girish Beedessee, Nadeem Nazurally, Abdulwahed Fahad Alrefaei and Rajesh Jeewon |
| Title | Phosphate solubilization potential of indigenous rhizosphere fungi and their biofertilizer formulations |
| Journal | Agronomy Research. 2021 |
| Authors | D.A. Balogun, M.A. Oke, L. Rocha-Meneses, OB. Fawole and P.F. Omojasola |
| Title | First Report of Sclerotinia sclerotiorum (Sclerotinia Blight) on Symphyotrichum dumosum in Poland |
| Journal | APS Publications. 2018 |
| Authors | K. Pieczul |
| Title | Cultivar-specific gene modulation in Vitis vinifera: analysis of the promoters regulating the expression of WOX transcription factors |
| Journal | Scientific Reports. 2017 |
| Authors | Boccacci P, Mela A, Pavez Mina C, Chitarra W, Perrone I, Gribaudo I, Gambino G. |
| Title | Lotus japonicus NF-YA1 plays an essential role during nodule differentiation and targets members of the SHI/STY gene family |
| Journal | Molecular plant-microbe interactions: MPMI. 2016. |
| Authors | Hossain MS, Shrestha A, Zhong S, Miri M, Austin RS, Sato S, Ross L, Huebert T, Tromas A, Torres-Jerez I, Tang Y, Udvardi M, Murray JD, Szczyglowski K. |
| Title | Numerical and RAPD Analysis of Eight Cowpea Genotypes from Nigeria |
| Journal | Nigerian Journal of Biotechnology. 2016. |
| Authors | E.O. Idehen, S.A. Oyelakin, A.O. Onikola, V.O. Adedapo |
| Title | Chemical Control of Black Scorch Disease on Date Palm Caused by the Fungal Pathogen, Thielaviopsis punctulata in United Arab Emirates |
| Journal | Plant Disease. 2016. |
| Authors | Esam Eldin Saeed, Arjun Sham, Khaled Abbas El-Tarabily, Firas Abu Elsamen, Rabah Iratni, Synan F. AbuQamar |
| Title | Identification Of Cuscuta Campestrisyuncker In Uae: Study Of Bar Code Loci-Rbcl, Matk And Trnh-Psbain The Uae And Egyptian Cultivars And In The Respective Host Plants Basil And Jute |
| Journal | International Journal of Research in Science. 2016. |
| Authors | Alsaadi, A., Saif, M., Yasser, M., Gaur, S., El-wakil, H., Maloukh, L., & Lakshmi, T. V. R |
| Title | Potential biosurfactant producing endophytic and epiphytic fungi, isolated from macrophytes in the Negro River in Manaus, Amazonas, Brazil |
| Journal | African Journal of Biotechnology. 2016. |
| Authors | Lima, J. M. S., Pereira, J. E. O., Batista, I. H., Neto, P. D. Q. C., dos Santos, J. C., de Ara&ujo, S. P., ... & de Azevedo, J. L. U |
| Title | Fate of transgenic deoxyribonucleic acid fragments in digesta and tissues of rabbits fed genetically modified soybean meal |
| Journal | Journal of Animal Science. 2015. |
| Authors | P. Morera, L. Basiricò, B. Ronchi and U. Bernabucci |
| Title | MiRNA-Mediated Regulation of the SWI/SNF Chromatin Remodeling Complex Controls Pluripotency and Endodermal Differentiation in Human ESCs |
| Journal | Stem Cells. 2015. |
| Authors | Wade SL, Langer LF, Ward JM, Archer TK |
| Title | Mangrove Rhizosphere Soils: A Unique Natural Source of Pravastatin-Producing Penicillium Microfungi |
| Journal | International Journal of Extensive Research. 2015. |
| Authors | Seydametova Emine, Norazwina Zainol, Jailani Salihon and Peter Convey |
| Title | A stable JAZ protein from peach mediates the transition from outcrossing to self-pollination |
| Journal | BMC Biology. 2015. |
| Authors | Sherif Sherif, Islam El-Sharkawy, Jaideep Mathur, Pratibha Ravindran, Prakash Kumar, Gopinadhan Paliyath and Subramanian Jayasankar |
| Title | Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues. |
| Journal | Applications in Plant Sciences. 2014. |
| Authors | Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey. |
| Title | Establishment of invasive and non-invasive reporter systems to investigate American elm–Ophiostoma novo-ulmi interactions. |
| Journal | Fungal Genetics and Biology. 2014. |
| Authors | S. Sherif, A.M.P. Jones, M.R. Shukla, P.K. Saxena. |
| Title | Aplification of TRP1 for Specific Detection of Phytophthora Ramorum. |
| Journal | United States Patent Application 022057. 2014. |
| Authors | Bohannon, Robert C., Russell Jr., Paul F. |
| Title | Development of a Real-Time Microchip PCR System for Portable Plant Disease Diagnosis. |
| Journal | PLoS One. 2013. |
| Authors | Koo C, Malapi-Wight M, Kim H, Cifci O, Vaughn-Diaz V, Ma B, Kim S, Abdel-Raziq H, Ong K, Jo Y, Gross D, Shim W, Han A |
| Title | Optimization of decolorization of palm oil mill effluent (POME) by growing cultures of Aspergillus fumigatus using response surface methodology. |
| Journal | Environmental Science and Pollution Research. 2012. |
| Authors | Neoh C, Yahya A, Adnan R, Majid Z and Ibrahim Z. |
| Title | Three sympatrically occurring species of Metarhizium show plant rhizosphere specificity. |
| Journal | Journal of Microbiology. 2011. |
| Authors | Wyrebek M, Huber C, Sasan RK, Bidochka MJ. |
| Title | Molecular cloning and expression analysis of a delta 6-fatty acid desaturase gene from Rhizopus stolonifer strain YF6 which can accumulate high levels of gamma-linolenic acid. |
| Journal | Journal of Microbiology. 2011. |
| Authors | Wan X, Zhang Y, Wang P, Jiang M. |
| Title | Flexible metabolism in Metarhizium anisopliae and Beauveria bassiana: utility of the glyoxylate cycle during insect pathogenesis |
| Journal | Microbiology. 2011. |
| Authors | Padilla-Guerrero IE, Barelli L, Gonzˆlez-Hernˆndez GA, Torres-Guzmˆn JC, Bidochka M. |
Plant/Fungi DNA Isolation Kits
These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
Plant/Fungi DNA Isolation Kit (Spin Column)
Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
Plant/Fungi DNA Isolation 96-Well Kit (HT)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well.
Plant/Fungi DNA Isolation Kit (Magnetic Bead System)
The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Plant/Fungi DNA Isolation 96-Well Kit (HT Magnetic Bead System)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Supporting Data
Kit Specifications
|
Kit Specifications - Spin Column
|
|
| Column Binding Capacity |
50 μg
|
| Maximum Column Loading Volume |
650 μL
|
|
Maximum Amount of Starting Material:
Plant Tissues
Fungi (wet weight)
|
100 mg
100 mg |
| Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) |
18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont'd) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
|
Kit Specifications - Spin Column
|
|
| Column Binding Capacity |
50 μg
|
| Maximum Column Loading Volume |
650 μL
|
|
Maximum Amount of Starting Material:
Plant Tissues
Fungi (wet weight)
|
100 mg
100 mg |
| Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) |
18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont'd) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
|
Kit Specifications - 96-well
|
|
| Binding Capacity Per Well |
50 μg
|
| Maximum Loading Volume Per Well |
500 μL
|
| Size of DNA Purified |
All sizes |
| Maximum Amount of Starting Material: Fungi Plant Tissues |
50 mg (wet weight) 50 mg |
| Average Yields* Grape leaf Apple leaf Strawberry leaf Plum leaf Peach leaf Peach petiole |
7 µg 3 µg 6 µg 4 µg 5 µg 4 µg |
| Time to Complete 96 Purifications |
50 minutes
|
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNase A which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont'd) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
|
Kit Specifications
|
|
|
Maximum Plant Input
|
50 mg
|
|
Type of Plants Processed
|
All types of plant species and tissue
|
|
Average Yield from 50 mg of Plant*
|
3-10 μg
|
| Average Purity (OD260/280) |
1.8 - 2.0
|
| Time to Complete 12 Purifications |
50 minutes
|
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
Store RNAse A at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
|
Kit Specifications
|
|
|
Maximum Plant Input
|
50 mg
|
|
Type of Plants Processed
|
All types of plant species and tissue
|
|
Average Yield from 50 mg of Plant*
|
3-10 μg
|
| Average Purity (OD260/280) |
1.8 - 2.0
|
| Time to Complete 96 Purifications |
Less than 60 minutes
|
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
Store RNase A at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 26200 (50 preps) | Cat. 26250 (250 preps) | Cat. 26900 (192 preps) | Cat. 58200 (50 preps) | Cat. 62400 (192 preps) |
|---|---|---|---|---|---|
| Lysis Buffer L | 30 mL | 1 x 30 mL 2 x 60 mL |
2 x 60 mL | 60 mL | 2 x 60 mL |
| Binding Buffer I | 7 mL | 1 x 7 mL 1 x 25 mL |
25 mL | 7 mL | 25 mL |
| Solution WN | 18 mL | 1 x 18 mL 1 x 55 mL |
55 mL | 18 mL | 55 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | - | - |
| Elution Buffer B | 15 mL | 30 mL | 30 mL | 8 mL | 30 mL |
| RNAse A | 1 vial (80 μL) | 5 vials (5 x 80 μL) | 1 vial | 1 vial | 1 vial |
| Magnetic Bead Suspension | - | - | - | 4 x 1.1 mL | 2 x 8.5 mL |
| Filter Columns | 50 | 250 | - | - | - |
| Spin Columns | 50 | 250 | - | - | - |
| Collection Tubes | 100 | 500 | - | - | - |
| 96-Well Plate | - | - | 2 | - | 2 |
| 96-Well Collection Plate | - | - | 2 | - | - |
| Adhesive Tape | - | - | 4 | - | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | - | 50 | - |
| 96-Well Elution Plate | - | - | 2 | - | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 |
Spin Column, HT (26200, 26250, 26900)
| Title | Leaf Bacteriome in Sugar Beet Shows Differential Response against Beet curly top virus during Resistant and Susceptible Interactions |
| Journal | Int. J. Mol. Sci . 2022 |
| Authors | Rajtilak Majumdar, Carl A. Strausbaugh, Eric D. Vincill, Imad Eujayl and Paul J. Galewski |
| Title | Chitosan Sensitivity of Fungi Isolated from Mango (Mangifera indica L.) with Anthracnose |
| Journal | Molecules . 2022 |
| Authors | Griselda Valenzuela-Ortiz, Soila Maribel Gaxiola-Camacho, Cesar San-Martín-Hernández, Miguel Ángel Martínez-Téllez, Emmanuel Aispuro-Hernández, Jaime Lizardi-Mendoza and Eber Addí Quintana-Obregón |
| Title | L-Asparaginase from Penicillium sizovae Produced by a Recombinant Komagataella phaffii Strain |
| Journal | Pharmaceuticals . 2022 |
| Authors | Marcela Freitas, Paula Souza, Mauricio Homem-de-Mello, Yris M. Fonseca-Bazzo, Damaris Silveira, Edivaldo X. Ferreira Filho, Adalberto Pessoa Junior, Dipak Sarker, David Timson, João Inácio and Pérola O. Magalhães |
| Title | Agaricus macrochlamys, a New Species from the (Sub)tropical Cloud Forests of North America and the Caribbean, and Agaricus fiardii, a New Synonym of Agaricus subrufescens |
| Journal | J. Fungi . 2022 |
| Authors | Rosario Medel-Ortiz, Roberto Garibay-Orijel, Andrés Argüelles-Moyao, Gerardo Mata, Richard W. Kerrigan, Alan E. Bessette, József Geml, Claudio Angelini, Luis A. Parra and Jie Chen |
| Title | Marker assisted selection (MAS) for downy mildew resistance in grapevines using Rpv3.1 associated markers |
| Journal | Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2022 |
| Authors | Murat AKKURT, Irem ŞENSES, Burak AKTÜRK, Ilhami TOZLU, Ilhami TOZLU and Halil Ibrahim UZUN |
| Title | New Antifungal Compound, 6-Pentyl-α-Pyrone, against the Maize Late Wilt Pathogen, Magnaporthiopsis maydis |
| Journal | Agronomy. 2022 |
| Authors | Ofir Degani, and Asaf Gordani |
| Title | Genomic analyses point to a low evolutionary potential of prospective source populations for assisted migration in a forest herb |
| Journal | Evolutionary Applications. 2022 |
| Authors | Frederik Van Daele,Olivier Honnay,Hanne De Kort |
| Title | Common facultative endosymbionts do not influence sensitivity of cereal aphids to pyrethroids |
| Journal | RES JOUNRALS. 2022 |
| Authors | Daniel J. Leybourne, Petra Melloh, Emily A. Martin |
| Title | Emergence and Genomic Characterization of Multidrug Resistant Candida auris in Nigeria, West Africa |
| Journal | J. Fungi. 2022 |
| Authors | Rita Oladele, Jessica N. Uwanibe, Idowu B. Olawoye, Abdul-Wahab O. Ettu, Jacques F. Meis and Christian T. Happi |
| Title | Alternaria species causing pomegranate and citrus fruit rots in Albania |
| Journal | Journal of Plant Diseases and Protection. 2022 |
| Authors | Magdalena Cara, Mirela Toska, Dajana Frasheri, Riccardo Baroncelli & Simona Marianna Sanzani |
| Title | Evaluation of Three Forms of Rhizoctonia solani Mediated Pathogenicity to Sugar Beet Cultivars in Greenhouse Studies |
| Journal | bioRxiv. 2022 |
| Authors | Md Ehsanul Haque, Most Shanaj Parvin |
| Title | Phylogeographic patterns in cryptic Bostrychia tenella species (Rhodomelaceae, Rhodophyta) across the Thai-Malay Peninsula |
| Journal | Algae. 2022 |
| Authors | Jakaphan Bulan, Sinchai Maneekat, Giuseppe C. Zuccarello, Narongrit Muangmai |