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Plasma/Serum cfc-DNA Advanced Purification Kit
Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit provides fast, reliable and simple procedures for isolating the highest quality and quantity of cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal genomic DNA (gDNA) contamination. Isolation is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and ct-DNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.
Kit Specifications
|
|
Minimum Plasma/Serum Input
|
500 µL
|
Maximum Plasma/Serum Input
|
6 mL
|
Optimal size of purified cfc-DNA
|
146 - 180 bp
|
Elution Volume |
25-50 µL
|
Time to Complete 10 Purifications |
45 minutes
|
*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.
Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.
Want a discount?
Create your account to receive 15% off on your first purchase!
Plasma/Serum cfc-DNA Advanced Purification Kit
Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit provides fast, reliable and simple procedures for isolating the highest quality and quantity of cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal genomic DNA (gDNA) contamination. Isolation is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and ct-DNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.
Kit Specifications
|
|
Minimum Plasma/Serum Input
|
500 µL
|
Maximum Plasma/Serum Input
|
6 mL
|
Optimal size of purified cfc-DNA
|
146 - 180 bp
|
Elution Volume |
25-50 µL
|
Time to Complete 10 Purifications |
45 minutes
|
*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.
Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.