Plasma/Serum cfc-DNA Advanced Purification Kit For rapid and simple purification of all sizes of cell-free circulating DNA (cfc-DNA) and circulating tumour DNA (ct-DNA) from plasma and serum samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plasma/Serum cfc-DNA Advanced Purification Kit For rapid and simple purification of all sizes of cell-free circulating DNA (cfc-DNA) and circulating tumour DNA (ct-DNA) from plasma and serum samples Price USD$1,035.00 SKU 68000 Format Spin Column Size 50 Preps Quantity Overview Details Documentation Citations Overview Versatile plasma and serum input volumes (0.5 mL - 6 mL) Concentrate cfc-DNA and ct-DNA into a flexible elution volume ranging from (25 µL - 50 µL) Minimal high molecular weight gDNA contamination in the purified cfc-DNA Isolate inhibitor-free cell-free circulating DNA Purify superior quantity and quality DNA in 45 minutes Compatible with fresh, preserved or frozen serum/plasma prepared from blood collected on either Norgen's cf-DNA/cf-RNA Preservative Tubes (Cat. 63950, 63960), Cell-Free DNA BCT ® (Streck), Heparin, EDTA or Citrate Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit provides fast, reliable and simple procedures for isolating the highest quality and quantity of cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal genomic DNA (gDNA) contamination. Isolation is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and ct-DNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS. Details Supporting Data Previous Figure 1. Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit was used to purify circulating DNA from 4 mL plasma from blood collected on EDTA as an anticoagulant from two different donors. Panel A and B shows the Bioanalyzer High Sensitivity DNA Chip trace for the cfc-DNA purified using Norgen’s kit as compared to Panel C and D representing the cfc-DNA purified by Competitor Q's kit which is a Magnetic Bead based kit. Norgen's kit showed the highest amount of the correct cfc-DNA size (Mono-nucleosomal cfc-DNA) with minimal high molecular weight gDNA contamination as opposed to the cfc-DNA profile obtained from Competitor Q's kit which showed low amount of the correct cfc-DNA with a bias toward binding higher molecular weight gDNA sizes. Figure 2. Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit was used to purify circulating DNA from 4 mL plasma from blood collected on EDTA as an anticoagulant from three different donors. The concentration of the Mono-nucleosomal cf-DNA (representing the correct cfc-DNA size) was measured using the Agilent Bioanalyzer High Sensitivity DNA Chip. Norgen's kit showed the highest Mono-nucleosomal cfc-DNA concentration with an average of 182.7 pg/µL ±13 as compared to the average concentration obtained from Competitor Q's kit which was 31.5 pg/µL ±3.1. Figure 3. Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit was used to purify circulating DNA from 1mL, 2mL, 4 mL and 6mL plasma prepared from blood collected on EDTA as an anticoagulant. 2 microlitres of the purified DNA was then used as the template in qPCR reactions to assess the linearity of the purified cfc-DNA by targeting a short ALU gene target (115 bp) representing the mono-nucleosomal cfc-DNA population. Norgen's Plasma/Serum cfc-DNA Advanced Purification Kit showed an excellent linearity between all plasma volumes with a % of linearity of more than 90% indicating efficient cfc-DNA recovery from all plasma volumes used. Next Click for expanded view Kit Specifications Minimum Plasma/Serum Input 500 µL Maximum Plasma/Serum Input 6 mL Optimal size of purified cfc-DNA 146 - 180 bp Elution Volume 25-50 µL Time to Complete 10 Purifications 45 minutes *Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods. Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature. Component Cat. 68000 (50 preps) Binding Buffer A 20 mL Proteinase K 6.5 mL Slurry E 12.5 mL Lysis Buffer A 130 mL Wash Solution A 38 mL Elution Buffer C 2 x 8 mL Elution Buffer F 1 x 15 mL1 x 6 mL Micro Spin Columns 50 Mini Filter Spin Columns 50 Collection Tubes 100 Elution Tubes (1.7 mL) 100 Product Insert 1 Documentation PROTOCOLS (68000) Plasma/Serum cfc-DNA Advanced Purification Kit - Protocol (50 Preps) FLYERS Non-Invasive Prenatal Testing (NIPT) Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Powered by Bioz See more details on Bioz Related Products cf-DNA/cf-RNA Preservative Tubes cf-DNA/cf-RNA Preservative Tubes Low Abundance DNA Quantification Kit
Plasma/Serum cfc-DNA Advanced Purification Kit For rapid and simple purification of all sizes of cell-free circulating DNA (cfc-DNA) and circulating tumour DNA (ct-DNA) from plasma and serum samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plasma/Serum cfc-DNA Advanced Purification Kit For rapid and simple purification of all sizes of cell-free circulating DNA (cfc-DNA) and circulating tumour DNA (ct-DNA) from plasma and serum samples Price USD$1,035.00 SKU 68000 Format Spin Column Size 50 Preps Quantity