Plasma/Serum cfc-DNA Advanced Purification Kit (Cat. 68000)

For rapid and simple purification of all sizes of cell-free circulating DNA (cfc-DNA) and circulating tumour DNA (ct-DNA) from plasma and serum samples
Versatile plasma and serum input volumes (0.5 mL - 6 mL)
Concentrate cfc-DNA and ct-DNA into a flexible elution volume ranging from (25 µL - 50 µL)
Minimal high molecular weight gDNA contamination in the purified cfc-DNA
Isolate inhibitor-free cell-free circulating DNA
Purify superior quantity and quality DNA in 45 minutes
Compatible with fresh, preserved or frozen serum/plasma prepared from blood collected on either Norgen's cf-DNA/cf-RNA Preservative Tubes (Cat. 63950, 63960), Cell-Free DNA BCT ® (Streck), Heparin, EDTA or Citrate
Fully automated Isolation procedure on Hamilton MicroLab Nimbus
Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Want a discount?

Create your account to receive 15% off on your first purchase!

Plasma/Serum cfc-DNA Advanced Purification Kit (Cat. 68000) 50 preps

Click for expanded view

Plasma/Serum cfc-DNA Advanced Purification Kit

Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit provides fast, reliable and simple procedures for isolating the highest quality and quantity of cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal genomic DNA (gDNA) contamination. Isolation is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and ct-DNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Kit Specifications
Minimum Plasma/Serum Input	500 µL
Maximum Plasma/Serum Input	6 mL
Optimal size of purified cfc-DNA	146 - 180 bp
Elution Volume	25-50 µL
Time to Complete 10 Purifications	45 minutes

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

Protocols:

68000 - Protocol

Posters:

Explore the World of RNA - Silicon Carbide vs Silica

Want a discount?

Create your account to receive 15% off on your first purchase!

Full Kits

Plasma/Serum cfc-DNA Advanced Purification Kit (Cat. 68000) 50 preps

Click for expanded view

Plasma/Serum cfc-DNA Advanced Purification Kit

Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit provides fast, reliable and simple procedures for isolating the highest quality and quantity of cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal genomic DNA (gDNA) contamination. Isolation is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and ct-DNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Kit Specifications
Minimum Plasma/Serum Input	500 µL
Maximum Plasma/Serum Input	6 mL
Optimal size of purified cfc-DNA	146 - 180 bp
Elution Volume	25-50 µL
Time to Complete 10 Purifications	45 minutes

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.