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Plasma/Serum cfc-DNA Advanced Purification Kit (Cat. 68000)

 

Purchasing Information

  • For rapid and simple purification of all sizes of cell-free circulating DNA (cfc-DNA) and circulating tumour DNA (ct-DNA)  from plasma and serum samples
  • Versatile plasma and serum input volumes (0.5 mL - 6 mL)
  • Concentrate cfc-DNA and ct-DNA into a flexible elution volume ranging from (25 µL - 50 µL)
  • Minimal high molecular weight gDNA contamination in the purified cfc-DNA
  • Isolate inhibitor-free cell-free circulating DNA
  • Purify superior quantity and quality DNA in 45 minutes
  • Compatible with fresh, preserved or frozen serum/plasma prepared from blood collected on either Norgen's cf-DNA/cf-RNA Preservative Tubes (Cat. 63950, 63960), Cell-Free DNA BCT ® (Streck), Heparin, EDTA or Citrate
  • Fully automated Isolation procedure on Hamilton MicroLab Nimbus

ItemsCat. #Size
Plasma/Serum cfc-DNA Advanced Purification Kit 68000 50 preps

  • For rapid and simple purification of all sizes of cell-free circulating DNA (cfc-DNA) and circulating tumour DNA (ct-DNA)  from plasma and serum samples
  • Versatile plasma and serum input volumes (0.5 mL - 6 mL)
  • Concentrate cfc-DNA and ct-DNA into a flexible elution volume ranging from (25 µL - 50 µL)
  • Minimal high molecular weight gDNA contamination in the purified cfc-DNA
  • Isolate inhibitor-free cell-free circulating DNA
  • Purify superior quantity and quality DNA in 45 minutes
  • Compatible with fresh, preserved or frozen serum/plasma prepared from blood collected on either Norgen's cf-DNA/cf-RNA Preservative Tubes (Cat. 63950, 63960), Cell-Free DNA BCT ® (Streck), Heparin, EDTA or Citrate
  • Fully automated Isolation procedure on Hamilton MicroLab Nimbus


Plasma/Serum cfc-DNA Advanced Purification Kit

Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit provides fast, reliable and simple procedures for isolating the highest quality and quantity of cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal genomic DNA (gDNA) contamination. Isolation is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and ct-DNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Kit Specifications
Minimum Plasma/Serum Input
500 µL
Maximum Plasma/Serum Input
6 mL
Optimal size of purified cfc-DNA
146 - 180 bp
Elution Volume
25-50 µL
Time to Complete 10 Purifications
45 minutes

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.


Protocols: 
68000 - Protocol
ItemsCat. #Size
Plasma/Serum cfc-DNA Advanced Purification Kit 68000 50 preps

  • For rapid and simple purification of all sizes of cell-free circulating DNA (cfc-DNA) and circulating tumour DNA (ct-DNA)  from plasma and serum samples
  • Versatile plasma and serum input volumes (0.5 mL - 6 mL)
  • Concentrate cfc-DNA and ct-DNA into a flexible elution volume ranging from (25 µL - 50 µL)
  • Minimal high molecular weight gDNA contamination in the purified cfc-DNA
  • Isolate inhibitor-free cell-free circulating DNA
  • Purify superior quantity and quality DNA in 45 minutes
  • Compatible with fresh, preserved or frozen serum/plasma prepared from blood collected on either Norgen's cf-DNA/cf-RNA Preservative Tubes (Cat. 63950, 63960), Cell-Free DNA BCT ® (Streck), Heparin, EDTA or Citrate
  • Fully automated Isolation procedure on Hamilton MicroLab Nimbus


Plasma/Serum cfc-DNA Advanced Purification Kit

Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit provides fast, reliable and simple procedures for isolating the highest quality and quantity of cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal genomic DNA (gDNA) contamination. Isolation is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and ct-DNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Kit Specifications
Minimum Plasma/Serum Input
500 µL
Maximum Plasma/Serum Input
6 mL
Optimal size of purified cfc-DNA
146 - 180 bp
Elution Volume
25-50 µL
Time to Complete 10 Purifications
45 minutes

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

Protocols: 
68000 - Protocol
 

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