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Plasma/Serum cfc-DNA Advanced Purification Kit
Versatile plasma and serum input volumes (0.5 mL - 6 mL)
Concentrate cfc-DNA and ct-DNA into a flexible elution volume ranging from (25 µL - 50 µL)
Minimal high molecular weight gDNA contamination in the purified cfc-DNA
Isolate inhibitor-free cell-free circulating DNA
Purify superior quantity and quality DNA in 45 minutes
Compatible with fresh, preserved or frozen serum/plasma prepared from blood collected on either Norgen's cf-DNA/cf-RNA Preservative Tubes (Cat. 63950, 63960), Cell-Free DNA BCT ® (Streck), Heparin, EDTA or Citrate
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit provides fast, reliable and simple procedures for isolating the highest quality and quantity of cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal genomic DNA (gDNA) contamination. Isolation is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and ct-DNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.
Figure 1. Norgens Plasma/Serum cfc-DNA Advanced Purification Kit was used to purify circulating DNA from 4 mL plasma from blood collected on EDTA as an anticoagulant from two different donors. Panel A and B shows the Bioanalyzer High Sensitivity DNA Chip trace for the cfc-DNA purified using Norgens kit as compared to Panel C and D representing the cfc-DNA purified by Competitor Q's kit which is a Magnetic Bead based kit. Norgen's kit showed the highest amount of the correct cfc-DNA size (Mono-nucleosomal cfc-DNA) with minimal high molecular weightgDNA contamination as opposed to the cfc-DNA profile obtained from Competitor Q's kit which showed low amount of the correct cfc-DNA with a bias toward binding higher molecular weightgDNA sizes.
Figure 2. Norgens Plasma/Serum cfc-DNA Advanced Purification Kit was used to purify circulating DNA from 4 mL plasma from blood collected on EDTA as an anticoagulant from three different donors. The concentration of the Mono-nucleosomal cf-DNA (representing the correct cfc-DNA size) was measured using the Agilent Bioanalyzer High Sensitivity DNA Chip. Norgen's kit showed the highest Mono-nucleosomal cfc-DNA concentration with an average of 182.7 pg/µL α13 as compared to the average concentration obtained from Competitor Q's kit which was 31.5 pg/µL α3.1.
Figure 3. Norgens Plasma/Serum cfc-DNA Advanced Purification Kit was used to purify circulating DNA from 1mL, 2mL, 4 mL and 6mL plasma prepared from blood collected on EDTA as an anticoagulant. 2 microlitres of the purified DNA was then used as the template in qPCR reactions to assess the linearity of the purified cfc-DNA by targeting a short ALU gene target (115 bp) representing the mono-nucleosomal cfc-DNA population. Norgen's Plasma/Serum cfc-DNA Advanced Purification Kit showed an excellent linearity between all plasma volumes with a % of linearity of more than 90% indicating efficient cfc-DNA recovery from all plasma volumes used.
*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.